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Device and method for testing activated partial thromboplastin time

A technology of thromboplastin time and activated part, which is applied in the field of devices for testing activated partial thromboplastin time, can solve the problems of cumbersome, laborious and time-consuming operations, and achieve the effects of simple operation, simple preparation method and broad prospects

Inactive Publication Date: 2018-06-12
北京乐普诊断科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the APTT test method is mainly based on liquid or powder reagents, and it is used with a fully automatic or semi-automatic coagulation instrument. The blood needs to be centrifuged and the plasma is extracted, and the operation is cumbersome. When using plasma to measure APTT, the plasma needs to be activated for a period of time. Then add APTT reagent to the activated blood sample, which is completed step by step, time-consuming and laborious

Method used

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  • Device and method for testing activated partial thromboplastin time
  • Device and method for testing activated partial thromboplastin time
  • Device and method for testing activated partial thromboplastin time

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Experimental program
Comparison scheme
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Embodiment 1

[0030] The reagent contained in reaction zone 1 can be divided into two parts: activator and APTT assay reagent. After the blood sample flows through the reaction zone 1, the blood sample is fully contacted and activated. Among APTT assay reagents, phospholipids and activators mainly play an active role. Among them, activators play a role in activating blood, and the source and type of phospholipids are very important to the final test results. Specifically, in this example, 0.5 mg ellagic acid, 1 mg phosphatidylserine, 0.8 mg phosphatidylethanolamine, 3.5 mg sodium chloride, 1.5 mg PEG 6000, 0.2 mg thimerosal were added to 10 mL of 50 mM HEPES buffer solution , after fully dissolving, set the pH to 7.0, and use it as a reagent to be added dropwise in reaction zone 1.

[0031] Reaction area 2 can be used as a quality control area, and only when the test results in the quality control area meet the experimental requirements after analysis, the final test results are considered...

Embodiment 2

[0034] The APTT test device prepared by the present invention has many differences from the APTT test kit, such as the state of the two active ingredients when they react with the blood sample to be tested. For the former, the active ingredient is evenly dispersed in the reaction area of ​​the substrate in the form of a solid film; for the latter, the reaction reagent exists in the form of liquid when participating in the reaction. The former reagent is to prepare a solid thin film in which the reagents are evenly dispersed on the substrate. The specific implementation method of the preparation is: add the reagent in the reaction zone 1, the reagent in the reaction zone 2 and the reagent in the reaction zone 3 dropwise into specific areas of the substrate (respectively attached figure 2 231, 232 and 233 shown in ), and dried at 70° C. for 10 min to obtain a substrate with attached reagents.

Embodiment 3

[0036]On the substrate (230) of the attached reagent prepared in Example 2, stick the double-sided adhesive tape (220) and the hydrophilic upper cover (210) successively (such as figure 2 shown). The schematic diagram of the device structure is attached figure 1 and 2 shown. 110 in the sample loading area is a sample loading hole, and 240 blood filter membranes are installed above 110, and its function is to separate plasma from blood cells. The reaction zones 112, 113 and 114 are respectively reaction zones 1, 2 and 3, and due to the supporting effect of the double-sided adhesive tape, the reaction zones 1, 2 and 3 have certain volumes. The topmost is the sample loading control area, wherein 115 is a stop node, and 116 is an air bag. It should be noted that the material at the corresponding positions of 112 and 116 on the 210 or 230 layer is an elastic material, which can be pressed manually or automatically by the equipment at the corresponding position. Under the actio...

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Abstract

The invention provides a device and a method for testing activated partial thromboplastin time (APTT) based on an optical method. The device is formed by a support, a chemical reagent and the like, wherein the support is formed by three parts: a sample adding area, a reacting area and a feeding control area. The chemical reagent is concretely prepared from an activating agent, phospholipid, CaCl2and other main components. The device for testing the APTT provided by the invention can be used for testing the APTT of fingertip blood or a non-coagulated blood sample, has the advantages of convenience, fastness, simplicity in operation and the like, is simple in preparation method, low in technical requirements on equipment and operating personnel, high in operability, and broad in applicationprospect in the fields such as clinic examination.

Description

technical field [0001] The invention relates to a device and method for blood sample testing, in particular to a device and method for testing activated partial thromboplastin time (APTT). Background technique [0002] Coagulation function testing is an important clinical testing item, which is of great significance for the diagnosis of various clinical diseases. The determination of blood coagulation function usually includes 4 inspection contents, namely prothrombin time (PT), activated partial thromboplastin time (APTT), thrombin time (TT) and fibrinogen (FIB). Among them, activated partial thromboplastin time (APTT) can be used to judge the state of endogenous coagulation activity, and is one of the main clinical coagulation performance test indicators. Completion of blood coagulation function tests including APTT is generally a must-check item before surgery, aiming to reduce intraoperative and postoperative risks. APTT can be used to confirm the deficiency of coagula...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/86
CPCG01N33/86
Inventor 刘芳胡飞邱笑违董飒英
Owner 北京乐普诊断科技股份有限公司
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