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Micro-ecological preparation and preparation method thereof

A technology of micro-ecological preparations and excipients, which is applied in food science, animal feed, additional food elements, etc., can solve the problem of insufficient resources of inactivated micro-ecological preparations, and achieve a stable effect

Inactive Publication Date: 2018-06-15
郑州神草生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved by the present invention is to provide a kind of probiotics and its preparation method in order to overcome the defect of insufficient resources of inactivated probiotics in the prior art

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] A microecological preparation and a preparation method thereof, each kilogram of the preparation contains: 450 grams of inactivated Bacteroides polymorpha and its fermentation products, wherein the content of inactivated Bacteroides polymorpha cells is 108-109 cells / g, and the specification is 80 A total of 550 grams of corn cob powder and precipitated silica, wherein each 225 grams of corn cob powder and precipitated silica.

[0029] A kind of probiotics and preparation method thereof, comprises the following steps:

[0030] S1. Fermentation of Bacteroides polymorpha: inoculate the fermentation medium with Bacteroides polymorpha with a seed age of 15 hours at an inoculum of 8% (v / v or v / w), and ferment at a temperature of 37°C , anaerobic culture for 48 hours; the fermentation medium is improved broth medium: improved broth medium pH7.0±0.2, 121 ° C autoclave for 15 minutes, when cooled to about 50 ° C, add filter-sterilized Vitamin K1 solution 0.5ml and hemin solutio...

Embodiment 2

[0036] A microecological preparation and a preparation method thereof, each kilogram of the preparation contains: 350 grams of inactivated Bacteroides fragilis and its fermentation product, wherein the content of inactivated Bacteroides fragilis cells is 108-109 cells / g, cornstarch and precipitated A total of 650 grams of silicon dioxide, wherein each 325 grams of cornstarch and precipitated silicon dioxide.

[0037] The preparation method of described probiotics, comprises the steps:

[0038] S1. Fermentation of Bacteroides fragilis: Inoculate the fermentation medium with 12-hour-old Bacteroides fragilis at an inoculum size of 5% (v / v or v / w), and anaerobically Cultivate for 40 hours; the fermentation medium is improved broth medium: improved broth medium pH7.0±0.2, autoclaved at 121°C for 15 minutes, when cooled to about 50°C, add filter-sterilized vitamin K1 solution 0.5ml and 2.5ml of hemin solution (5mg / ml), mix well and set aside.

[0039] S2. After the fermentation is...

Embodiment 3

[0044] A microecological preparation and a preparation method thereof, each kilogram of the preparation contains: 480 grams of inactivated Bacteroides fragilis and Bacteroides polymorpha and their fermentation products, wherein the total cell content of inactivated Bacteroides fragilis and Bacteroides polymorpha is 108 ~109 pieces / g, the specification is 80 mesh corncob powder and precipitated silica, 520 grams in total, of which corncob powder and precipitated silica are 260 grams each.

[0045] As mentioned above, a kind of probiotics and preparation method thereof: comprising the following steps:

[0046] S1. Separate fermentation culture of Bacteroides fragilis and Bacteroides polymorpha: Bacteroides fragilis and Bacteroides polymorpha with a seed age of 16 hours were respectively inoculated into the fermentation medium at an inoculation amount of 8% (v / v or v / w), Under the condition of fermentation temperature of 37°C, anaerobic culture was carried out for 48 hours; the f...

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Abstract

The invention relates to the field of micro-ecological preparations, in particular to a micro-ecological preparation and a preparation method thereof. The preparation is prepared from inactivated bacteroides and fermented concentrate as main components, and the micro-ecological preparation containing the inactivated bacteroides has the efficacy of resisting bacteria, resisting inflammation, regulating gastrointestinal microbial flora and preventing human and animal gastrointestinal diseases, and has the characteristics of quick effect, high safety and convenient use. The preparation should beused for disease prevention and health care of human and animals, has good practical application value, and is especially outstanding when applied to animal feed. Therefore, the probiotic inactivatedmicro-ecological preparation is a technology which is worthy of popularization.

Description

technical field [0001] The invention relates to the field of probiotics, in particular to a probiotic and a preparation method thereof. Background technique [0002] Bacteroides are a large number of normal flora in humans and animals, accounting for more than 1 / 4 of the intestinal flora, and maintain a special environment for the intestinal tract with other intestinal flora and the host, which plays a significant role in the health of the host to an important role. Bacteroides can help the host decompose polysaccharides, form intestinal mucosal blood vessels, improve host immunity, and maintain intestinal microecological balance. There are many types of Bacteroides in the intestinal tract, which together constitute the intestinal Bacteroides flora, coordinate and complement each other to maintain the balance of the intestinal flora. Therefore, the effect of preparing a compound Bacteroides probiotics will be more significant than that of a single Bacteroides probiotics. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K10/18A23K10/20A23K10/30A23K20/10A23K20/163A23K20/20A23K40/00A23K40/10A23K40/30A23L33/135
CPCA23K10/18A23K10/20A23K10/30A23K20/10A23K20/163A23K20/20A23K40/00A23K40/10A23K40/30A23L33/135
Inventor 不公告发明人
Owner 郑州神草生物技术有限公司
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