Fluorescence collection and analysis method for array type microfluidic chip

Abstract

The invention discloses fluorescence collection and analysis method for an array type microfluidic chip and belongs to the technical field of micro total analysis. According to the method, a deflection angle of a whole chip can be calculated by cross marks on the edge of the chip, and corresponding rotation operation can be performed according to the calculated angle during post processing of eachpicture, so that tedious steps and time consumption caused by manual operational measurement are avoided; reaction microchamber parts are easy to distinguish according to intervals, and with adoptionof a partitioned image collection and analysis method, errors caused by splicing and other factors are avoided and original nature of each image is reserved; the problem about fluorescence information detection of the array type microfluidic chip biological reaction is solved, and a foundation is laid for a rapid digital microfluid diagnosis system.

Description

technical field [0001] The invention belongs to the technical field of micro-total analysis, in particular to a fluorescence collection and analysis method of an array microfluidic chip. Background technique [0002] Nowadays, the fluorescence conversion of biological information has become an important method in the field of life science research, and the application of microfluidic chips in biological research is becoming more and more extensive. Functions are transferred to portable analysis devices, such as various chips. In the microfluidic chip analysis system, samples are placed on integrated chips of several square centimeters to achieve high-throughput and low-cost chemical analysis. [0003] The patent No. CN1548944A introduces a microfluidic chip laser-induced fluorescence analyzer. The pinhole of the instrument is equipped with a replaceable CCD camera. It communicates with a computer to obtain image signals. The computer controls the stepping motor and can be se...

AI Technical Summary

Problems solved by technology

The instrument uses laser light to obtain fluorescence information, and the laser wavelength has a certain range, which will inevitably limit the selection of fluorescent materials; for some experiments that require the shortest time to obtain the reaction results after some biological reactions, the instrument will also be delayed in time, which may be lost. Important information after the reaction; and the micro-reaction chamber of some high-density microfluidic chips may be too small, and it is impossible to obtain clear images using only CCD

[0004] The patent with the application number 201710789184.8 proposes a new automatic fluorescence signal acquisition and analysis method based on dPCR. The patent collects images in different regions of the dPCR chip, automatically recognizes the splicing and counts the fluorescence intensity, and realizes full automation. However, the acquisition It takes a long time to make the theoretical position coincide with the actual position in each step of the picture process; when automatically identifying and splicing, the method of first row and second column is adopted to separate the pictures between rows; when extracting the fluorescence intensity, first according to the design size Create the same matrix, and then extract the data of the corresponding position on the photo according to the one-to-one correspondence

This method solves the fluorescence detection of some microfluidic chips to a certain extent, but for the fluorescence detection of some microfluidic chips, there is a problem that the pictures cannot be collected accurately and clearly; and when the divided area increases, the system moves and misplaces, which will cause some problems when splicing. A certain error occurs

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