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A method for cultivating Cordyceps militaris with living Tenebrio molitor larvae as host

A technology of Tenebrio molitor and Cordyceps militaris, which is applied in the field of artificial cultivation of rare medicinal fungi, can solve the problems of difficult control of the inoculation site and inoculation amount, and achieve good medicinal health care value, short cycle and significant benefits

Active Publication Date: 2020-04-03
TAIAN ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] In view of the deficiencies in the prior art, especially the selection of insect age, inoculation site and inoculation amount are not easy to control, the present invention provides a method for cultivating Cordyceps militaris with living Tenebrio molitor larvae as host, so as to realize the live artificial cultivation of Cordyceps militaris, Improve medicinal value, partially replace Cordyceps sinensis, protect wild resources of Cordyceps sinensis, and promote the development and utilization of Cordyceps militaris

Method used

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  • A method for cultivating Cordyceps militaris with living Tenebrio molitor larvae as host

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] A method for cultivating Cordyceps militaris with living Tenebrio molitor larvae as a host, comprising the following steps:

[0047] (1) Bacterial liquid preparation

[0048] Use a 500mL Erlenmeyer flask to hold 300mL of liquid medium. The nutrients contained in the liquid medium are: 20g of glucose, 5g of peptone, 3g of yeast extract, and vitamin B per liter of water. 1 0.5mg, peanut oil 0.5mL; 0.11MPa, 121℃ steam sterilization for 40min, cooled to room temperature, insert 4 pieces of 1mm under sterile conditions 2 Age-appropriate Cordyceps militaris slant strains, cultured statically for 24 hours, cultured on a constant temperature shaker at 160r / min, 24°C for 5 days, the dry weight of mycelium reached 500mg / 100mL, and crushed to a particle size of 200-300μm under sterile conditions of bacteria liquid.

[0049] (2) Culture carrier

[0050] Add 150g of fine sand and 15g of water into a 500mL transparent container, stir evenly, seal with polypropylene film, steriliz...

Embodiment 2

[0064] A method for cultivating Cordyceps militaris with living Tenebrio molitor larvae as a host, comprising the following steps:

[0065] (1) Bacterial solution preparation

[0066] Use a 500mL Erlenmeyer flask to hold 300mL of liquid medium. The nutrients contained in the liquid medium are: 20g of glucose, 5g of peptone, 3g of yeast extract, and vitamin B per liter of water. 1 0.5mg, peanut oil 0.5mL; 0.11MPa, 121℃ steam sterilization for 40min, cooled to room temperature, insert 4 pieces of 1.5mm under sterile conditions 2 Age-appropriate Cordyceps militaris slant strains, cultured statically for 24 hours, cultured on a constant temperature shaker at 160r / min, 24°C for 5 days, the dry weight of mycelium reached 500mg / 100mL, and crushed to a particle size of 200-300μm under sterile conditions of bacteria liquid.

[0067] (2) Culture carrier

[0068] Add 150g of fine sand and 20g of water into a 500mL transparent container, stir evenly, seal with polypropylene film, ster...

Embodiment 3

[0082] A method for cultivating Cordyceps militaris with living Tenebrio molitor larvae as a host, comprising the following steps:

[0083] (1) Bacterial liquid preparation

[0084] Use a 500mL Erlenmeyer flask to hold 300mL of liquid medium. The nutrients contained in the liquid medium are: 20g of glucose, 5g of peptone, 3g of yeast extract, and vitamin B per liter of water. 1 0.5mg, peanut oil 0.5mL; 0.11MPa, 121℃ steam sterilization for 40min, cooled to room temperature, insert 4 pieces of 1.5mm under sterile conditions 2 Age-appropriate Cordyceps militaris slant strains, cultured statically for 24 hours, cultured on a constant temperature shaker at 160r / min, 24°C for 5 days, the dry weight of mycelium reached 500mg / 100mL, and crushed to a particle size of 200-300μm under sterile conditions of bacteria liquid.

[0085] (2) Culture carrier

[0086] Add 150g of fine sand and 22.5g of water into a 500mL transparent container, stir well, seal with polypropylene film, steril...

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Abstract

The invention belongs to the technical field of artificial cultivation of rare medical fungi and relates to a method for culturing cordyceps militaris by taking living tenebrio molitor larvae as hosts. According to the method, the living tenebrio molitor larvae are taken as the hosts, inoculation of cordyceps militaris liquid is performed by artificial injection, and the contaminated living tenebrio molitor larvae are ossified slowly along with growth of in-vivo hyphae to grow out cordyceps militaris fruiting bodies through culturing. By adoption of the method, the contamination rate of the tenebrio molitor larvae reaches 85% or above, the ossification rate reaches 80% or above, and the cordyceps militaris is rich in content of effective components including cordyceps polysaccharide, cordycepin and cordycepic acid. The method is simple in operating process, high in yield and low in cost, cultured products are rich in nutrition and high in medicinal value, and suitableness for large-scale cultivation and a promising application prospect are realized.

Description

technical field [0001] The invention relates to a method for cultivating Cordyceps militaris with living larvae of Tenebrio molitor as a host, and belongs to the technical field of artificial cultivation of rare medicinal fungi. Background technique [0002] With people's understanding of the health care function of Cordyceps sinensis, the demand has increased sharply, the market resources are tight, and the price is high. Due to its limited natural conditions and limited output, it is far from meeting the growing demand of domestic and foreign markets; Mining seriously damages the natural vegetation and ecological balance. Therefore, Cordyceps militaris, a substitute for Cordyceps sinensis, is developed to meet the needs of domestic and foreign markets and protect the ecological environment. [0003] Cordyceps militaris (Cordyceps militaris), also known as Cordyceps militaris, Cordyceps militaris, etc., has been tested by authoritative departments and contains unique medici...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G18/00A01G18/40
Inventor 王庆武唐丽娜兰玉菲李秀梅丛倩倩崔晓安秀荣蔚承祥
Owner TAIAN ACAD OF AGRI SCI
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