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A method for preparing neoastilbin with Smilax cocos as raw material

A technology for new astilbin and tuckahoe, which is applied in the field of preparation of new astilbin and can solve the problems of lack and the like

Active Publication Date: 2020-10-30
JIANGI WENIR NUTRITION HIGH TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The present invention aims at the technical defects of the prior art, and provides a method for preparing neoastilbin with Smilax cocos as a raw material, so as to solve the technical problem of lacking a method of this type in the prior art

Method used

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  • A method for preparing neoastilbin with Smilax cocos as raw material
  • A method for preparing neoastilbin with Smilax cocos as raw material
  • A method for preparing neoastilbin with Smilax cocos as raw material

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Embodiment 1

[0031] 1 kg of Smilax smilax was crushed and passed through a 40-mesh sieve, 20 L of 50% ethanol aqueous solution was added, stirred and extracted at room temperature for 60 minutes, and the extract was obtained by conventional filtration. figure 1 High performance liquid chromatography for Smilax smilax extract. In the figure, peak 1 is astilbin, and peak 2 is astilbin. As determined by high performance liquid chromatography, the content of astilbin in Smilax cocos is 2.2%, and the content of neoastilbin is 0.17%, that is, 1kg of Smilax cocos contains 22g of astilbin and 1.7g of neoastilbin; The content of astilbin was the highest, while the content of astilbin was very low.

[0032] Concentrate the Smilax smilax extract to remove ethanol, adjust the pH to 8, add ascorbic acid with a final concentration of 1 mM, and place it in an 80-degree water bath for 10 hours to carry out isomerization reaction. figure 2 It is high performance liquid chromatography of Smilax smilax ex...

Embodiment 2

[0037] (1) Take 1 kg of Smilax cocos and crush it through a 40-mesh sieve, add 20 L of 60% ethanol solution, stir and extract at room temperature for 60 minutes, and filter through a 200-mesh filter cloth to obtain the extract.

[0038] (2) The temperature of the extract obtained in step (1) is concentrated under vacuum at 60 degrees, and the ethanol is recovered to obtain 4L of the concentrated solution; as determined by high performance liquid chromatography, the concentrated solution contains 22 g of astilbin and 1.7 g of astilbin in total. g.

[0039] (3) After adjusting the pH of the concentrated solution obtained in step (2) to 7 with 1mol / L NaOH solution, add 0.704g of ascorbic acid, the final concentration of ascorbic acid is 1mM, and place it in an 80°C water bath for 10 hours to carry out isomerization reaction. As determined by high performance liquid chromatography, the concentrated solution now contained 3.9 g of astilbin and 16.7 g of astilbin in total. The conc...

Embodiment 3

[0043] A method for preparing neoastilbin with Smilax cocos as raw material, comprising the following steps:

[0044] 1) get Smilax smilax, cross 20 mesh sieves after pulverizing, take the ethanol aqueous solution that concentration is 10% (v / v) as extractant, be 1:20 ( kg:L), the sieve was mixed with the extractant, stirred at room temperature for 60min, filtered, and the filtrate was taken;

[0045] 2) concentrating the filtrate obtained in step 1) in a vacuum at 60° C., recovering ethanol, and obtaining a concentrated solution;

[0046] 3) The pH of the concentrated solution obtained in step 2) was adjusted to 5, and ascorbic acid was added thereto to a final concentration of 0.1 mM, and left to react at 50° C. for 16 hours.

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Abstract

The invention provides a method for preparing astilbin from Smilax smilax as raw material. The technical scheme first investigated the interconversion relationship between the four isomers of astilbin in Smilax smilax, and unexpectedly found that in the presence of ascorbic acid, specific pH and temperature conditions can initiate the isomerization of astilbin to astilbin reaction, based on this beneficial discovery, the present invention is guided by the isomerization direction, conversion degree, product decomposition degree, etc., and has explored the best reaction conditions; under this condition, astilbin is mainly isomerized into astilbin Neoturoside, and the decomposition rate is slower. Based on this, the present invention further designs the front-end extraction conditions and subsequent purification methods. By applying the method of this invention, most of the astilbin in Smilax cocos can be converted into neoastilbin, thereby increasing the content of neoastilbin in the Smilax smilax extract by nearly 9 times, which greatly improves the separation and purification process from Smilax smilax. Efficiency of neoastilbin.

Description

technical field [0001] The invention relates to the technical field of extraction of plant active components, in particular to a method for preparing neoastilbin from Smilax smilax as raw material. Background technique [0002] Astilbin, namely 2(R):3(R)-3',4',5,7-4 hydroxysulfanone-3-α-L-rhamnopyranose, is the main effect of the traditional Chinese medicine Smilax cocos Element. Astilbin has a variety of biological activities, such as selective immunosuppression, regulation of fat metabolism, anti-oxidation, and liver protection. [0003] According to the molecular structure of astilbin, it has stereoisomerism at the C2 and C3 positions, respectively 2(R):3(R) (astilbin), 2(S):3(S) (astilbin glycoside), 2(S):3(R) (neoisoastilbin) and 2(R):3(S) (isoastilbin) four isomers. The isomerism of compounds may affect their biological activity, and the two enantiomers of some drugs have completely different pharmacological effects. Therefore, it is necessary to study the biologic...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07H17/07C07H1/08
CPCC07H1/08C07H17/07
Inventor 张清峰郑丹
Owner JIANGI WENIR NUTRITION HIGH TECH
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