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Macrofluid-based microfluidic chip and preparation method thereof

A microfluidic chip and chip control technology, applied in chemical instruments and methods, foam dispersion/prevention, laboratory utensils, etc., can solve the problems of limiting the application of fluidic chips, complex micron structure manufacturing process, etc., and achieve balanced polishability , excellent printing effect, and balanced mechanical properties

Inactive Publication Date: 2018-06-29
张策
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although there are many molding methods, the pressure valve necessary to control the fluid for complex operations (peristaltic pump, automatic liquid delivery, proportioning, etc.) is integrated in the chip, and the scale of the fluid chip is extended to millimeters or even for biopathological tissue sample manipulation. The centimeter scale is still a key issue in the research of microfluidic chips
Moreover, the manufacturing process of the micron structure used for the PDMS chip template is complex (including ultra-clean space, micron structure preparation equipment, etc.), and the bonding conditions of the multilayer functional structure of the chip based on plasma oxidation are easily affected by the external environment (such as the air environment, Chemical gases, etc.) and other issues also largely limit the application of fluidic chips in biomedicine

Method used

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  • Macrofluid-based microfluidic chip and preparation method thereof
  • Macrofluid-based microfluidic chip and preparation method thereof
  • Macrofluid-based microfluidic chip and preparation method thereof

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Effect test

Embodiment 1

[0040] Step 1, Preparation of PLA positive mold of PDMS microfluidic macrofluidic chip

[0041] 1.1 Use 3D printing PLA polysmooth material to prepare the microfluidic macrofluidic chip control layer and the positive mold of the fluidic layer;

[0042] 1.2 Put the positive mold in alcohol spray for surface polishing, control layer for 30 minutes, fluid layer for 3 hours;

[0043] 1.3 Clean the control layer and fluid layer;

[0044] combine figure 1 , which is a schematic diagram of the 3D printed polysmooth block structure transformed into a smooth arched structure suitable for a fluidic chip pressure valve after alcohol droplet polishing. After 3D printing, the printing material polysmooth has a block structure. After alcohol droplet polishing, the block structure disappears and turns into a smooth arched structure. The surface of the 3D printed model is smoother. This smooth structure is suitable for fluid chips. Use of pressure valves.

[0045] combine figure 2 , whi...

Embodiment 2

[0059] combine Figure 5 , which is a schematic diagram of the preparation process and application of the PDMS fluidic chip in step 2.

[0060] in Figure 5 (A) Fluid chip preparation process based on 3D printing and polishing technology: 1. Combining the semi-cured PDMS film layer with the fluid control layer; 2. Heating to 80°C for stable bonding; 3. Making different ratios of PDMS and curing agent 4. Using the super-proportional combination method to integrate the different layer structures of the fluid, and complete the chip preparation. Figure 5 (B) A fluid chip with four culture chambers with a diameter of 4.5 mm and a height of 1.5 mm fabricated using this technique. By controlling the switch of the entrance and exit of each culture chamber, different culture environments can be constructed in the 4 culture chambers, so as to carry out complex biological experiments on cell nucleus biological tissues. The related functions are demonstrated by using food coloring age...

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Abstract

The invention discloses a preparation method of a macrofluid-based microfluidic chip. The method comprises the following steps: preparing male molds of a fluid layer and a control layer of a microfluidic chip from novel material polysmooth; smoothing surface structures of the male molds through alcohol spray; preparing a PDMS chip control layer and a PDMS chip fluid layer with a molding method; preparing a pressure regulation film by spinning PDMS to surface of a blank silicon chip; combining the chip fluid layer with the PDMS film through half-curing; combining the control layer with the fluid layer provided with the PDMS film through off-ratio; performing punching and sealing the bottom of the chip with a PCR adhesive tape to obtain the macrofluid-based microfluidic chip. Physiological,pathological and drug screening tests on micro scale (cells and bacteria) and macro scale (tissue sections) can be realized simultaneously with adoption of the microfluidic chip.

Description

technical field [0001] The invention relates to the technical field of microfluidic chips, in particular to a microfluidic macrofluidic control chip and a preparation method thereof, in particular to a method for preparing a PDMS microfluidic macroscopic fluidic control chip based on alcohol polishing polymer materials and 3D printing technology. Background technique [0002] Microfluidics integrates the basic operation units such as sample preparation, reaction, separation, and detection in the biological, chemical, and medical analysis processes into a micron-scale chip to automatically complete the entire analysis process. Mainly taking advantage of its small size, by constructing miniature reaction and analysis units on the surface of solid chips, it can realize rapid and accurate detection of inorganic ions, organic substances, nucleic acids, proteins and other biochemical components, and can handle picometer, nanometer Level fluid, so it has the advantages of high auto...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01L3/00B01D19/02B01J19/00
CPCB01D19/02B01J19/00B01L3/502707B01L3/502753B01L2200/027B01L2300/0887B01L2300/12
Inventor 张策
Owner 张策
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