A kind of zinc-rich bacterial strain and its application

A technology of strains and bacteria powder, applied in zinc-rich strains and its application fields, can solve problems such as limiting market development space, and achieve the effect of promoting the activities of various digestive enzymes in the intestine, low cost, and promoting intestinal digestion

Active Publication Date: 2020-06-12
山东德信生物科技有限公司
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  • Abstract
  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

However, this kind of product with a single characteristic of the application strain greatly limits the development space of the market

Method used

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  • A kind of zinc-rich bacterial strain and its application
  • A kind of zinc-rich bacterial strain and its application
  • A kind of zinc-rich bacterial strain and its application

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preparation example Construction

[0035] 6) Preparation of Bacillus subtilis zinc: Cultivate the domesticated Bacillus subtilis under the final optimized culture medium and culture conditions, after culturing for a certain period of time, centrifuge and wash to obtain a precipitate, and then dry it at 55°C to obtain Bacillus subtilis zinc.

[0036] The preparation parameters of Bacillus subtilis zinc are: ① Medium formula: peptone 10g / L, yeast powder 5g / L, NaCl 10g / L, glucose 4.50g / L, zinc concentration 33.9mg / L, pH 6.57; ② Culture conditions: The inoculum size is 3%, the culture temperature is 29.50°C, the culture time is 48 hours, and the rotation speed is 180r / min.

[0037] When the amount of zinc added was 33.9 mg / kg, the dry weight of the finally obtained Bacillus subtilis zinc was 1.44193 g / L, and the organic zinc content of the bacteria was 2511.55 mg / kg.

[0038] The method of the present invention will be described in detail below in conjunction with specific implementation cases.

Embodiment 1

[0039] Embodiment 1: the preparation of Bacillus subtilis zinc

[0040] 1) Domestication of strains: Bacillus subtilis strains are obtained through activation, separation and purification of Bacillus subtilis powder, which is commonly used in feed addition; The mode of zinc sulfate concentration in the culture medium is domesticated;

[0041] 2) Identification of strains and physical and chemical properties: identify the strains of Bacillus subtilis after preliminary domestication, and compare the morphology, enzyme activity, small peptides, amino acids, etc. with the strains before domestication to further determine the strains of subtilis after domestication Bacillus was the target species.

[0042]The final screening obtained Bacillus subtilis NZ56 strain, which was preserved on January 31, 2018 at No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing, China Microbiological Culture Collection Management Committee, Institute of Microbiology, Chinese Academy of Sc...

Embodiment 2

[0054] The establishment of embodiment 2 fermentation method

[0055] 1, the steps of the present invention are as follows:

[0056] 1) Determination of the balance point (measuring index): according to the growth characteristics of the strain and the tolerance to metal zinc ions, determine the bacterial content and the organic zinc content of the bacterial cell as the final measuring index.

[0057] 2) Optimization of culture medium and culture conditions: The content of glucose, pH, culture temperature, rotation speed, inoculum amount, etc. were optimized through single factor experiments, and the culture medium formula and culture conditions of the strain were preliminarily obtained.

[0058] 3) Response surface analysis: According to the single factor results, further determine the medium formula and culture conditions of the strain through response surface analysis.

[0059] 4) Preparation of Bacillus subtilis zinc: Cultivate the domesticated Bacillus subtilis under the ...

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Abstract

The present invention relates to a kind of zinc-enriched bacterial strain and its application. The method adopted in the present invention gradually increases the concentration of zinc sulfate to domesticate Bacillus subtilis to obtain Bacillus subtilis strain, which can significantly improve the tolerance of Bacillus subtilis, not only increase The zinc content of the bacteria is improved, and the content of crude protein, enzymes, small peptides, amino acids, etc. in the bacteria is increased, so that the microecology and organic zinc are integrated, easy to use, and good application effect. The prepared Bacillus subtilis zinc not only has the advantages of Bacillus subtilis, but also converts inorganic zinc into bacterial organic zinc, which greatly improves the utilization rate of trace metal elements in animals and better exerts the biological functions of organic zinc. In addition, the improvement of the utilization rate of organic zinc greatly reduces the use and discharge of inorganic zinc in feed, and reduces the pollution of the ecological environment. For this reason, this invention provides important technical support for the development of new animal microecological preparations and the "reduced" use of trace elements.

Description

technical field [0001] The invention belongs to the technical field of production of livestock and poultry microecological preparations, and in particular relates to a zinc-rich bacterial strain and application thereof. Background technique [0002] Bacillus subtilis is a beneficial intestinal bacterium with strong stress resistance, can secrete a variety of enzymes and vitamins, can enrich divalent metal ions, is not easily affected by various enzymes in the intestines of livestock and poultry, and has the ability to enhance the immunity of livestock and poultry. Strength and anti-stress ability, regulating intestinal flora, improving growth performance and other functions. It is mainly used in the field of animal feed and environmental protection. Studies have shown that adding Bacillus subtilis powder to animal feed can significantly increase animal feed intake and various growth indicators; on the other hand, using the characteristics of Bacillus subtilis that can adsor...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12P9/00C12R1/125
CPCC12N1/20C12P9/00C12N1/205C12R2001/125
Inventor 王宝维黄燕萍葛文华张名爱岳斌
Owner 山东德信生物科技有限公司
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