Optical coding library, preparation method of carriers thereof, and application of optical coding library and carriers thereof

An optical encoding and carrier technology, applied in the field of suspension arrays, can solve problems such as limited encoding level, limited encoding capacity, and complicated steps

Active Publication Date: 2018-06-29
HANGZHOU JOINSTAR BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the method in this patent has the following defects: 1. The fluorescence intensity variation coefficient (CV) of the host microspheres prepared by the swelling method is large, so the fluorescent intensity adjustment range of the identifiable and distinguishable encoded microspheres is narrow, resulting in the main body Fluorescent microspheres are encoded at a limited level that can be decoded and identified by flow cytometry
And the microsphere brightness is not high
These directly lead to the weak coding ability of the host microspheres, and the low coding capacity of the final host-guest microspheres
2. Coding signals are limited to color coding
Multi-color coding is performed by using fluorescent dye

Method used

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  • Optical coding library, preparation method of carriers thereof, and application of optical coding library and carriers thereof

Examples

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Effect test

preparation example Construction

[0083] 2. Preparation method of medium fluorescence intensity guest moiety (MF)

[0084] Step 1: ultrasonically dissolve 5.25 mg of fluorescein isothiocyanate (FITC) in 1 mL of absolute ethanol, stir magnetically at 300 rpm, add 73 μL of APTES, and react in the dark for 12 hours to obtain a FITC-APS solution.

[0085] Step 2: Take a three-necked flask, add 24.4mL of absolute ethanol, 1.6mL of ultrapure water and 1.3mL of 25% ammonia water in sequence, stir magnetically at 500rmp and add 100μL of the FITC-APS solution in step 1, react for 10min, then add 2.0mL of TEOS, React in a water bath at 40 degrees Celsius for 3 hours in the dark. After the reaction, centrifuge (10,000 rpm, 15 min) to discard the supernatant, wash once with ultrapure water, and redisperse the precipitate in 25 mL of water to obtain a FITC-CORE dispersion.

[0086] Step 3: Dilute 15mL of 25% ammonia water in 85mL of ultrapure water to make solution A, add 124.2mL of absolute ethanol and 25mL of the FITC-CO...

Embodiment 1

[0100] Example 1 (both 0.5 μm and 2.9 μm host moieties combined with 2-fold guest moieties):

[0101] 1. Using magnetic microspheres with a diameter of 0.5 μm as the core matrix (the first part), the main part of the quantum dot code is prepared according to the above-mentioned main part preparation steps by the improved layer-by-layer assembly method. Using different quantum dot concentrations and quantum dot assembly layers, the main part of the 2-fold fluorescent quantum dot code is obtained, that is, Z 1 =2.

[0102] Two kinds of guest moieties with different fluorescence intensities (which can be blank fluorescence intensity guest moieties and medium fluorescence intensity guest moieties) prepared by the guest moiety preparation method were mixed according to different ratios, and then assembled into 0.5 μm The surface of the body part. By regulating the mixing ratio of these two guest moieties, a combination of guest moieties with 2-fold FITC encoding is obtained, that...

Embodiment 2

[0106] Example 2 (both 2.9 μm and 6.2 μm host moieties combined with 3-fold guest moieties):

[0107] 1. Using magnetic microspheres with a diameter of 2.9 μm as the core matrix (the first part), the main part of the quantum dot code is prepared by the improved layer-by-layer assembly method according to the above-mentioned preparation steps of the main part. Using different quantum dot concentrations and quantum dot assembly layers, the main part of the 8-fold fluorescent quantum dot code is obtained, that is, Z 1 =8.

[0108] Three kinds of guest moieties with different fluorescence intensities (which can be a blank fluorescence intensity guest moiety, a medium fluorescence intensity guest moiety and a high fluorescence intensity guest moiety) prepared by the guest moiety preparation method are mixed according to different proportions of these three kinds of guest moieties, Then assemble to the surface of the main part of 2.9 μm. By regulating the mixing ratio of these thr...

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Abstract

The invention discloses an optical coding library. The optical coding library comprises carriers with N sizes, wherein N is greater than or equal 2; each carrier comprises a subject part and object parts which are connected with the subject part; the subject part and at least one of the object parts comprise a fluorescent element; the central emission wavelength of the fluorescent element on the subject part is different from the central emission wavelength of the fluorescent element on each object part. The invention also discloses a preparation method of the carriers in the optical coding library, and the application of the carriers and the optical coding library. The optical coding library has ultrahigh coding capacity, and has the characteristics of high coding accuracy and high repeatability in application.

Description

technical field [0001] The invention relates to a suspension array for multi-index detection, in particular to a preparation method and application of an optical coding library and its carrier. Background technique [0002] With the development of post-genomics, faced with the acquisition and analysis of massive biological information, people have an increasingly urgent need for fast and efficient multi-index detection technology, and suspension array technology is currently one of the most suitable multi-index detection technologies for clinical applications. The core element of the suspension array is the coded microspheres used to identify, capture and manipulate the target molecules to be tested. Whether the target molecules can be coded, identified and decoded in a simple, convenient and controllable way is the core issue of the preparation technology of the coded microspheres. . Among different encoding methods, optical encoding combined with flow cytometry-based deco...

Claims

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Application Information

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IPC IPC(8): C40B50/10B82Y30/00B82Y20/00B82Y25/00B82Y40/00C09K11/06C09K11/02C09K11/00
CPCC09K11/00C09K11/02C09K11/06C40B50/10B82Y20/00B82Y25/00B82Y30/00B82Y40/00
Inventor 徐宏古宏晨鲁思洪龙斌周旭一
Owner HANGZHOU JOINSTAR BIOTECH
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