Optical coding library, preparation method of carriers thereof, and application of optical coding library and carriers thereof

Abstract

The invention discloses an optical coding library. The optical coding library comprises carriers with N sizes, wherein N is greater than or equal 2; each carrier comprises a subject part and object parts which are connected with the subject part; the subject part and at least one of the object parts comprise a fluorescent element; the central emission wavelength of the fluorescent element on the subject part is different from the central emission wavelength of the fluorescent element on each object part. The invention also discloses a preparation method of the carriers in the optical coding library, and the application of the carriers and the optical coding library. The optical coding library has ultrahigh coding capacity, and has the characteristics of high coding accuracy and high repeatability in application.

Description

technical field [0001] The invention relates to a suspension array for multi-index detection, in particular to a preparation method and application of an optical coding library and its carrier. Background technique [0002] With the development of post-genomics, faced with the acquisition and analysis of massive biological information, people have an increasingly urgent need for fast and efficient multi-index detection technology, and suspension array technology is currently one of the most suitable multi-index detection technologies for clinical applications. The core element of the suspension array is the coded microspheres used to identify, capture and manipulate the target molecules to be tested. Whether the target molecules can be coded, identified and decoded in a simple, convenient and controllable way is the core issue of the preparation technology of the coded microspheres. . Among different encoding methods, optical encoding combined with flow cytometry-based deco...

AI Technical Summary

Problems solved by technology

However, the method in this patent has the following defects: 1. The fluorescence intensity variation coefficient (CV) of the host microspheres prepared by the swelling method is large, so the fluorescent intensity adjustment range of the identifiable and distinguishable encoded microspheres is narrow, resulting in the main body Fluorescent microspheres are encoded at a limited level that can be decoded and identified by flow cytometry

And the microsphere brightness is not high

These directly lead to the weak coding ability of the host microspheres, and the low coding capacity of the final host-guest microspheres

2. Coding signals are limited to color coding

Multi-color coding is performed by using fluorescent dyes of different colors. However, due to the spectral interference between fluorescent dyes, the types of colors that can be used are limited, so the coding capacity that can be achieved is also limited.

3. The layer-by-layer assembly method that can be used to prepare the host microsphere (the first part) and the guest microsphere (sub-sphere) mentioned in the patent example is carried out by electrostatic interaction using water-soluble quantum dots, and the steps of the method are complicated. The fluorescent properties of quantum dots are easily damaged during the preparation process, and the cost of water-soluble quantum dots is high, which will eventually lead to high production costs of encoded microspheres

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