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Extraction method and application of Coridius chinensis chitosan

An extraction method and technology of nine incense insects, applied in the application, animal feed, additional food elements, etc., can solve problems such as fewer solutions, and achieve the effects of less impurities, shortening extraction time, and improving resource utilization.

Inactive Publication Date: 2018-07-03
FOSHAN JUCHENG BIOCHEM TECH RES & DEV CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Therefore, Chitosan from Nine Fragrance Worm has a high extraction value, but searching for the extraction of Nine Fragrance Worm can reveal that there are few schemes related to chitosan

Method used

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  • Extraction method and application of Coridius chinensis chitosan

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] A method for extracting chitosan from Nine Fragrance Worm, comprising the steps of:

[0027] (1) Collect the worm residue after protein extraction, add an appropriate amount of dilute hydrochloric acid to soak for 2 hours, then add 15 times the amount of acetic acid, soak for 1.5 days, heat up to 50°C, and perform ultrasonic-vacuum extraction. The ultrasonic power is 750W , the extraction time is 4h, the vacuum degree is 0.07MPa, the extract is centrifuged at a speed of 4500r / min, and the time is 13min, and the supernatant is collected;

[0028] (2) Ultrafiltration is performed on the supernatant collected in step (1), the membrane inlet pressure is 15 bar, the flux is 4L / min, and the material temperature is 25° C. After removing small molecular substances, the ultrafiltrate of Jiuxiangworm is obtained;

[0029] (3) dropwise addition of mass fraction to the Jiuxiangworm ultrafiltrate in step (2) is 25% H 2 o 2 Solution, keep warm in a water bath at 63°C for 4 hours, a...

Embodiment 2

[0032] A method for extracting chitosan from Nine Fragrance Worm, comprising the steps of:

[0033] (1) Collect the worm residue after protein extraction, add an appropriate amount of dilute hydrochloric acid to soak for 1 hour, then add 10 times the amount of acetic acid, soak for 1 day, heat up to 45°C, and perform ultrasonic-vacuum extraction. The ultrasonic power is 650W. The extraction time is 3h, the vacuum degree is 0.05MPa, the extract is centrifuged at 4000r / min, the time is 10min, and the supernatant is collected;

[0034] (2) Ultrafiltration is performed on the supernatant collected in step (1), the membrane inlet pressure is 15 bar, the flux is 3L / min, and the material temperature is 25°C. After removing small molecular substances, the ultrafiltrate of Nine Fragrance Insect is obtained;

[0035] (3) dropwise addition of mass fraction to the Jiuxiangworm ultrafiltrate in step (2) is 25% H 2 o 2 Solution, keep warm in a water bath at 60°C for 3 hours, and then carr...

Embodiment 3

[0038] A method for extracting chitosan from Nine Fragrance Worm, comprising the steps of:

[0039] (1) Collect the worm residue after protein extraction, add an appropriate amount of dilute hydrochloric acid to soak for 3 hours, then add 20 times the amount of acetic acid, soak for 2 days, heat up to 60°C, and perform ultrasonic-vacuum extraction. The ultrasonic power is 800W. The extraction time is 5h, the vacuum degree is 0.08MPa, the extract is centrifuged at a speed of 5000r / min, and the time is 15min, and the supernatant is collected;

[0040] (2) Ultrafiltration is performed on the supernatant collected in step (1), the membrane inlet pressure is 15 bar, the flux is 5 L / min, and the material temperature is 25° C. After removing small molecular substances, the ultrafiltrate of Nine Fragrance Insect is obtained;

[0041] (3) dropwise addition of mass fraction to the Jiuxiangworm ultrafiltrate in step (2) is 25% H 2 o 2 Solution, keep warm in a water bath at 65°C for 5 h...

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Abstract

The invention discloses an extraction method and application of Coridius chinensis chitosan, and further comprises a formula for applying the Coridius chinensis chitosan to feed of Uvarovites inflatus. The extraction method of Coridius chinensis chitosan comprises the following general steps of: performing soaking in dilute hydrochloric acid, adopting acetic acid as a solvent, extracting components by using an ultrasonic-vacuum technology, filtering supernatant, performing decolorization, and finally performing deacetylation treatment by using an alkaline liquid so as to obtain the Coridius chinensis chitosan. Through extraction the chitosan from Coridius chinensis, natural resources can be utilized effectively, the resource consumption rate can be reduced, and meanwhile an effect of improvement of a body immunity function can be achieved after the Coridius chinensis chitosan is made into the feed of Uvarovites inflatus.

Description

technical field [0001] The invention relates to a method for extracting chitosan, in particular to a method for extracting chitosan from wormwood. Background technique: [0002] Nine-scented insects, commonly known as spanking insects, fart insects, etc., belong to the family Pentatomidae of Hemiptera and are important medicinal insects. Its shell is rich in chitosan. [0003] Chitosan is known as "the sixth element of human life" by scientists. It can laxative the intestines, promote digestion, eliminate waste and toxic substances in the digestive tract, and clean up heavy metal ions to prevent and reduce the harm of heavy metal toxins to human organs. Because chitosan is also rich in a large number of biologically active "enzymes", it has significant effects on lowering blood sugar, blood lipids, blood pressure, etc., and has been widely recognized by the medical community. [0004] Chitosan can inhibit the activity of fat-related enzymes, prevent lipase from promoting ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/08A23K50/90A23K20/163A23K10/20A23K20/147A23K10/30
CPCA23K10/20A23K10/30A23K20/147A23K20/163A23K50/90C08B37/0003
Inventor 不公告发明人
Owner FOSHAN JUCHENG BIOCHEM TECH RES & DEV CO LTD
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