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Membrane-type metalloprotease inhibitors and uses thereof

A metalloprotease and inhibitory protein technology, applied in the field of membrane-type metalloprotease inhibitory protein, can solve the problems of limited value and no inhibitory effect

Active Publication Date: 2021-08-06
XIAN JIAOTONG LIVERPOOL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Disadvantages of the existing protein (i.e. the original TIMP-1): (1) The original TIMP-1 is a soluble secreted protein
(2) TIMP-1 has no inhibitory effect on MT1-MMP and TACE
(3) TIMP-1 can enhance cell activity, which greatly limits its value in cancer research

Method used

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  • Membrane-type metalloprotease inhibitors and uses thereof
  • Membrane-type metalloprotease inhibitors and uses thereof
  • Membrane-type metalloprotease inhibitors and uses thereof

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Experimental program
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Embodiment Construction

[0039] The specific embodiments of the present invention will be described below in conjunction with the accompanying drawings.

[0040] 1. Materials

[0041] 1. Experimental animals

[0042] NOD-SCID mice (Beijing Weitong Lihua Experimental Animal Technology Co., Ltd.)

[0043] 2. Drugs and reagents:

[0044] 2.1 Drugs: puromycin

[0045] 2.2 Reagents: TIMP-1 antibody (R&D), MT1-MMP antibody (Abcam), TACE antibody (Abcam), AlexaFluor488 anti-mouse secondary antibody (Invitrogen), Alexa Fluor555 anti-rabbit secondary antibody (Invitrogen), DAPI mounting medium (Invitrogen) ), 38% paraformaldehyde (Sigma), green fluorescent gelatin (ThermoScientific: Oregon green488), lipofectamine transfection kit (Invitrogen), propylene glycol methyl ether acetate (PMA), phosphate substrate (Sigma), TNF-α Elisa Kit (Sino Bio), EcoR I endonuclease (ThermoScientific), Xho I endonuclease (ThermoScientific), Apa I endonuclease (ThermoScientific), Nde I endonuclease (ThermoScientific), related...

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Abstract

The invention belongs to the technical field of biomedicine, and the invention claims protection of a membrane-type metalloprotease inhibitory protein T1 PrαTACE , the present invention also discloses membrane-type metalloproteinase inhibitor protein T1 PrαTACE The use of the method can be used to prepare drugs targeting MT1‐MMP or TACE endoproteinase.

Description

technical field [0001] The invention relates to the technical field of biomedicine, and specifically relates to a membrane-type metalloproteinase inhibitory protein capable of inhibiting the proliferation of human kidney cancer cells. Background technique [0002] Membrane Type 1-Matrix MetalloProteinase (Membrane Type 1-Matrix MetalloProteinase, MT1-MMP) and TNF-α converting enzyme TACE (TNF-αConverting Enzyme, TACE) are the two most widely studied endoproteinases. These two proteases are located on the surface of the cell membrane, and in the presence of zinc ions, they can regulate the concentration of extracellular proteins and other biologically active substances, thereby promoting the growth, proliferation, invasion and metastasis of cancer cells. For normal cells, the enzymatic activities of MT-MMP and TACE are tightly regulated and inhibited by endogenous cell metalloproteinase inhibitors (Tissue Inhibitors of MetalloProteinase, TIMPs). There are currently four know...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/81A61K38/57A61P35/00
CPCA61K38/00C07K14/8146
Inventor 李明辉姜冰洁
Owner XIAN JIAOTONG LIVERPOOL UNIV
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