Sagittaria sagittifolia Linn polysaccharide composition capable of enhancing immunologic function and preparation method of sagittaria sagittifolia Linn polysaccharide

A technology for enhancing immunity and composition, which can be used in pharmaceutical combinations, medical preparations containing active ingredients, food science, etc., can solve the problem that the immunomodulatory activity has not yet been reported, and achieve easy industrialization and amplification, no environmental pollution, and simple methods. Effect

Inactive Publication Date: 2018-07-27
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Modern pharmacological studies have shown that Sagittarius has various effects such as anti-oxidation, detoxification, hypoglycemia, and anti-tumor, but the research on its immunomodulatory activity and its active ingredients has not been reported yet.

Method used

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  • Sagittaria sagittifolia Linn polysaccharide composition capable of enhancing immunologic function and preparation method of sagittaria sagittifolia Linn polysaccharide

Examples

Experimental program
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Effect test

Embodiment 1

[0031] Preparation of Cigu polysaccharide: After removing impurities, washing and slicing fresh Cigu, immediately add water with a mass-to-volume ratio of 1:10 (g:mL) to make a slurry. The slurry is passed through a 60 mesh industrial filter cloth to obtain a filtrate. The filtrate is subjected to pressure The pump is injected into the subcritical extraction kettle, the magnetic coupling stirrer is controlled to 300 revolutions / min, the extraction pressure is 1MPa, the temperature is 100 ℃, and the extraction is 10 minutes. After the end, centrifuge and collect the supernatant; concentrate the supernatant to 1 / 2 of the original volume ; Add 4 times its volume of 95% ethanol to the concentrated solution, leave it for 24 hours, and centrifuge to separate the precipitate; after the precipitate is reconstituted, the DEAE-52 cellulose that has been balanced on it, first eluted with deionized water, and then It is eluted with 0.1% NaCl solution with mass fraction, and the salt eluate ...

Embodiment 2

[0034] Preparation of Cigu polysaccharide: After removing impurities, washing and slicing fresh Cigu, immediately add water with a mass-to-volume ratio of 1:10 (g:mL) to make a slurry. The slurry is passed through a 60 mesh industrial filter cloth to obtain a filtrate. The filtrate is subjected to pressure The pump is injected into the subcritical extraction kettle, the magnetic coupling stirrer is controlled at 450 rpm, the extraction pressure is 6 MPa, the temperature is 140 ℃, and the extraction is 10-30 min. After the end, centrifuge and collect the supernatant; the supernatant is concentrated to 1 of the original volume / 2; Add 4 times its volume of 95% ethanol to the concentrated solution, leave it to stand for 24 hours, and centrifuge to separate the precipitate; after the precipitate is re-dissolved, the DEAE-52 cellulose that has been balanced on it, first eluted with deionized water , And then eluted with 0.1% NaCl solution with mass fraction, collected the salt eluate...

Embodiment 3

[0037] Preparation of Cigu polysaccharide: After removing impurities, cleaning and slicing fresh Cigu, immediately add water with a mass-to-volume ratio of 1:10 (g:mL) to make a slurry. The slurry is passed through a 60 mesh industrial filter cloth to obtain a filtrate. The filtrate is passed through a pressure pump Inject into the subcritical extraction kettle, control the magnetic coupling stirrer at 600 rpm, the extraction pressure is 12MPa, the temperature is 180°C, and the extraction is 30min. After the end, centrifuge and collect the supernatant; concentrate the supernatant to 1 / 2 of the original volume; Add 4 times the volume of 95% ethanol to the concentrated solution. After standing for 24 hours, the precipitate is separated by centrifugation; after the precipitate is re-dissolved, the DEAE-52 cellulose that has been balanced is loaded, first eluted with deionized water, and then used The mass fraction of 0.1% NaCl solution is eluted, and the salt eluate is collected an...

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Abstract

The invention discloses a sagittaria sagittifolia Linn polysaccharide composition capable of enhancing an immunologic function and a preparation method of the sagittaria sagittifolia Linn polysaccharide composition, and relates to the technical field of medicine and agricultural product processing. The sagittaria sagittifolia Linn polysaccharide composition is prepared from 10 to 50 parts by massof sagittaria sagittifolia Linn polysaccharide and accessories, wherein the accessories are prepared from the following auxiliary components in parts by mass: 5 to 10 parts of dextrin, 5 to 20 parts of starch, 1 to 5 parts of beewax and 1 to 5 parts of dodecyl sulfuric acid, and the sagittaria sagittifolia Linn polysaccharide composition is prepared according to the proportions, can be prepared into oral preparations such as powder, capsules, tablets and granules, and can be used for enhancing the immunologic function. Effective parts and effective components, for enhancing the immunologic function, of sagittaria sagittifolia Linn are cleared, and a new resource is provided for accurately developing medicine or nutritional healthcare products having an immunoregulation function from a sagittaria sagittifolia Linn resource. The invention provides the preparation method of the sagittaria sagittifolia Linn polysaccharide composition capable of enhancing the immunologic function. The preparation method provided by the invention is simple, has no environmental pollution and is easy in industrialized enlargement.

Description

Technical field [0001] The invention belongs to the technical field of medicine and agricultural products processing, and specifically relates to a Cigu polysaccharide composition for enhancing immune function and a preparation method thereof. Background technique [0002] Sagittaria sagittifolia Linm (Sagittaria sagittifolia Linm), also known as wild rice and mushroom, is a perennial shallow-water herb of the genus Sagittifolia of the Alisma family. Our country is the place of origin of Ci aunts. It is dominated by southern water towns, and most of them are cultivated. In particular, Cigu has a large planting area and good quality in Baoying County, Yangzhou City, Jiangsu Province. It is known as the "Hometown of Ci aunts in China" and is regarded as a national geographic location. Logo product. The edible bulbs of Cigu are rich in starch, protein, polysaccharides, vitamins, iron, calcium, zinc and other trace elements needed by the human body. Its average nutritional value is ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/715A61K36/884A61P37/04A23L33/125
Inventor 段玉清陈梦张海晖罗孝平张继贤张迪任晓锋
Owner JIANGSU UNIV
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