RNA (Ribonucleic Acid) adapters of anti-viral envelope glycoprotein for inhibiting filovirus infection and application of RNA adapter

A technology of enveloped glycoproteins and filoviruses, applied in the field of biology, can solve problems such as sequence differences

Inactive Publication Date: 2018-07-27
ACADEMY OF MILITARY MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The amino acid homology of the Zaire-Ebola virus envelope glycoprotein from Sierra Leone reached 100% in 2014, but the amino acid sequences of the three Zaire-Ebola virus glycoproteins from Guinea have certain differences

Method used

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  • RNA (Ribonucleic Acid) adapters of anti-viral envelope glycoprotein for inhibiting filovirus infection and application of RNA adapter
  • RNA (Ribonucleic Acid) adapters of anti-viral envelope glycoprotein for inhibiting filovirus infection and application of RNA adapter
  • RNA (Ribonucleic Acid) adapters of anti-viral envelope glycoprotein for inhibiting filovirus infection and application of RNA adapter

Examples

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Embodiment 1

[0043] Embodiment 1, obtaining the RNA aptamer of the antiviral envelope glycoprotein (Glycoprotein, GP) that can inhibit filovirus (Ebola virus and Marburg virus) infection

[0044] The present invention obtains the RNA aptamer of antiviral envelope glycoprotein (Glycoprotein, GP) that can inhibit filovirus (Ebola virus and Marburg virus) infection, comprising the following steps:

[0045] 1) Obtain the stable conformation of the target protein: obtain the three-dimensional structure of the filovirus envelope glycoprotein from the NCBI database (3CSY, such as figure 1 shown), the molecular dynamics simulation is carried out by GROMACS (5.1releases) software, and after the molecular dynamics track is stable, the stable conformation of the target protein is selected (such as figure 2 shown) for subsequent analysis.

[0046] 2) Artificial design of aptamer sequence and structure: based on systems and synthetic biology methods, on the basis of designing various biological regul...

Embodiment 2

[0065] Example 2. Obtaining the preferred RNA aptamer SKLPBT1 of the antiviral envelope glycoprotein (Glycoprotein, GP) that can inhibit the infection of filoviruses (Ebola virus and Marburg virus)

[0066] The preferred method for obtaining the RNA aptamer (taking SKLPBYT1 as an example) of the antiviral envelope glycoprotein (Glycoprotein, GP) infected by filoviruses (Ebola virus and Marburg virus) of the present invention comprises the following steps :

[0067] 1) Obtaining a stable conformation of the target protein: the same as in Example 1.

[0068] 2) Artificial design of aptamer sequence and structure: the same as in Example 1.

[0069] 3) Screening of candidate aptamers: According to the structural characteristics in the stable conformation of the target protein, combined with the aptamer library generated by simulation, select a candidate aptamer (SKLPBY1) that can specifically bind to the designed regions (A and E) . Use the mFold software to obtain the secondar...

Embodiment 3

[0073] Example 3. Obtaining the preferred RNA aptamer SKLPBT2 of the antiviral envelope glycoprotein (Glycoprotein, GP) that can inhibit the infection of filoviruses (Ebola virus and Marburg virus)

[0074] The preferred method for obtaining the RNA aptamer (taking SKLPBT2 as an example) of the antiviral envelope glycoprotein (Glycoprotein, GP) infected by filoviruses (Ebola virus and Marburg virus) of the present invention comprises the following steps :

[0075] 1) Obtaining a stable conformation of the target protein: the same as in Example 1.

[0076] 2) Artificial design of aptamer sequence and structure: the same as in Example 1.

[0077] 3) Screening of candidate aptamers: According to the structural characteristics in the stable conformation of the target protein, combined with the aptamer library generated by simulation, select a candidate aptamer (SKLPBY2) that can specifically bind to the designed regions (A and E) . Use the mFold software to obtain the secondary...

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Abstract

The invention discloses RNA (Ribonucleic Acid) adapters of anti-viral GP (Glycoprotein) for inhibiting filovirus (Ebola virus and Marburg virus) infection and application of the RNA adapter. CandidateRNA adapters are all called SEQ ID SKLPBY and are respectively named from SKLPBY1 to SKLPBY5, and target RNA adapters are all called SEQ ID SKLPBT and are respectively named from SKLPBT1 to SKLPBT5.The RNA adapters disclosed by the invention are capable of specifically combining GP of filoviruses and inhibiting the infection efficiency of the filoviruses, and can be used as a diagnosis reagent and an inhibitor for filovirus infection.

Description

technical field [0001] The invention belongs to ribonucleotide (RNA) and its sequence and structure in the field of biology, in particular to a kind of antiviral envelope glycoprotein ( Glycoprotein, GP) RNA aptamer and its sequence, structure and its application in the preparation of diagnostic reagents and therapeutic drugs for filovirus infection. Background technique [0002] Filoviruses are single-stranded negative-sense RNA viruses belonging to the family Filoviridae, including Ebola virus, Marburg virus and Cueva virus. Ebola virus and Marburg virus are major and severe infectious diseases that can endanger public safety and human health, and are listed as Class A biological and chemical weapons. Ebola virus and Marburg virus can cause outbreaks of highly fatal hemorrhagic fever, and there are no effective antiviral treatments and vaccines against these two viruses. Among them, Ebola virus is the most prevalent filovirus, which can be transmitted among people throug...

Claims

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Application Information

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IPC IPC(8): C12N15/115G01N33/569A61K31/7105A61P31/14
CPCA61K31/7105C12N15/115C12N2310/16G01N33/56983
Inventor 滕越童贻刚刘书霞米志强安小平
Owner ACADEMY OF MILITARY MEDICAL SCI
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