ATP (adenosine triphosphate) fluorescence detection reagent with remarkable heat stability

A technology of thermal stability and fluorescence detection, which is applied in the field of ATP fluorescence detection, can solve the problems of poor thermal stability and short storage time, and achieve the effect of enhancing thermal stability and prolonging storage time

Inactive Publication Date: 2018-07-27
XIAMEN HAIHONGXING INSTR
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] Aiming at the deficiencies in the prior art, the present invention provides an ATP fluorescence detection reagent with remarkable thermal stability, which solves the problems of poor thermal stability and short storage time of existing commercially available ATP fluorescence detection kits

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  • ATP (adenosine triphosphate) fluorescence detection reagent with remarkable heat stability

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Embodiment 1

[0019] A fluorescent detection reagent for ATP with remarkable thermal stability. A specific ratio of thermal stability enhancing reagent is added to the enzyme reaction basic solution system and buffer solution. The ATP fluorescent detection reagent consists of three parts: the basic enzyme reaction solution system and the buffer system And heat stability enhancing reagent, enzyme reaction basic solution system comprises luciferase, luciferin and metal ion, luciferase is preferably luciferase, luciferin is preferably D-luciferin potassium salt, luciferase and The concentration of D-luciferin potassium salt is preferably 10mg / L and 40mg / L respectively, the metal ion is Mg2+, the concentration of metal ion Mg2+ is preferably 12.5mM, and the buffer system is preferably a glycylglycine buffer system, glycylglycine The concentration of the buffer system is preferably 25mM, the pH of the glycylglycine buffer system is 7.8, and the thermal stability enhancing reagent includes the fol...

Embodiment 2

[0021] A fluorescent detection reagent for ATP with remarkable thermal stability. A specific ratio of thermal stability enhancing reagent is added to the enzyme reaction basic solution system and buffer solution. The ATP fluorescent detection reagent consists of three parts: the basic enzyme reaction solution system and the buffer system And heat stability enhancing reagent, enzyme reaction basic solution system comprises luciferase, luciferin and metal ion, luciferase is preferably luciferase, luciferin is preferably D-luciferin potassium salt, luciferase and The concentration of D-luciferin potassium salt is preferably 10mg / L and 40mg / L respectively, the metal ion is Mg2+, the concentration of metal ion Mg2+ is preferably 12.5mM, and the buffer system is preferably a glycylglycine buffer system, glycylglycine The concentration of the buffer system is preferably 25mM, the pH of the glycylglycine buffer system is 7.8, and the thermal stability enhancing reagent includes the fol...

Embodiment 3

[0023]A fluorescent detection reagent for ATP with remarkable thermal stability. A specific ratio of thermal stability enhancing reagent is added to the enzyme reaction basic solution system and buffer solution. The ATP fluorescent detection reagent consists of three parts: the basic enzyme reaction solution system and the buffer system And heat stability enhancing reagent, enzyme reaction basic solution system comprises luciferase, luciferin and metal ion, luciferase is preferably luciferase, luciferin is preferably D-luciferin potassium salt, luciferase and The concentration of D-luciferin potassium salt is preferably 10mg / L and 40mg / L respectively, the metal ion is Mg2+, the concentration of metal ion Mg2+ is preferably 12.5mM, and the buffer system is preferably a glycylglycine buffer system, glycylglycine The concentration of the buffer system is preferably 25mM, the pH of the glycylglycine buffer system is 7.8, and the thermal stability enhancing reagent includes the foll...

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Abstract

The invention discloses an ATP (adenosine triphosphate) fluorescence detection reagent with remarkable heat stability. A specific proportion of heat stability enhancing reagent is added to an enzyme reaction basic solution system and a buffer solution; the ATP fluorescence detection reagent comprises the enzyme reaction basic solution system, a buffer system and the heat stability enhancing reagent, wherein the enzyme reaction basic solution system contains luciferase, fluorescein and metal ions, and preferably, luciferase is firefly luciferase, fluorescein is D-luciferin potassium salt, concentrations of firefly luciferase and D-luciferin potassium salt are 10 mg/L and 40 mg/L respectively; metal ions are Mg<2+> and concentration of the metal ions Mg<2+> is 12.5 mM preferably. According to the ATP fluorescence detection reagent with remarkable heat stability, the heat stability of the detection reagent is greatly enhanced, reagent activity can still be maintained in a certain period of time under the condition of extreme high temperature being 75 DEG C, and therefore, the storage time of the detection reagent is remarkably prolonged.

Description

technical field [0001] The invention relates to the technical field of ATP fluorescence detection, in particular to an ATP fluorescence detection reagent with remarkable thermal stability. Background technique [0002] Food is closely related to people's life and health. Therefore, ensuring the quality of food cannot be ignored. my country's current food safety problems mainly focus on food adulteration and microbial contamination. The total number of bacteria is a sign of the degree of food contamination. The total number of bacteria directly reflects the degree of contamination of food and can be used to predict the possible storage period of food. Therefore, the total number of bacteria is one of the important bases for judging the hygienic quality of food. The traditional bacteriological testing method uses the plate counting method, which is accurate and reliable, but the operation is complex and time-consuming. With the development of molecular biology, microelectr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/66
CPCC12Q1/66
Inventor 江秋菊蔡庆国杨德辉杨彬
Owner XIAMEN HAIHONGXING INSTR
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