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Magnetic particle reagent and kit for chemiluminescence immunodetection

A chemiluminescence immune, magnetic particle technology, applied in chemiluminescence/bioluminescence, analysis by chemical reaction of materials, measurement devices, etc., can solve problems such as poor stability, easy aggregation of magnetic particles, and inability to quickly mix. Achieve the effect of not easy to aggregate, ensure protein activity, and good preservation effect

Active Publication Date: 2018-08-07
SUZHOU HYBIOME BIOMEDICAL ENG CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0004] The technical problem to be solved by the present invention is: in order to solve the technical problems that the existing magnetic particle reagents used in chemiluminescent immunoassays have poor stability under adverse environments such as high temperature, and the magnetic particles are easy to aggregate but cannot be mixed quickly, and provide a method for Magnetic particle reagents and kits for chemiluminescent immunoassays

Method used

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  • Magnetic particle reagent and kit for chemiluminescence immunodetection
  • Magnetic particle reagent and kit for chemiluminescence immunodetection
  • Magnetic particle reagent and kit for chemiluminescence immunodetection

Examples

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Embodiment 1-11 and comparative example 1-4

[0028] Embodiments 1-11 and Comparative Examples 1-4 provide streptavidin-coated magnetic particle reagents for chemiluminescence immunoassays, and the magnetic particle reagents contain the following components:

[0029]

[0030]

[0031]

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Abstract

The invention relates to a magnetic particle reagent and a kit for chemiluminescence immunodetection. The magnetic particle reagent comprises magnetic particles coated by 0.5-1.5mg / mL streptavidin oranti-fluorescein isothiocyanate antibody, 0.5-5mg / mL protein, 0.5-10mg / mL chloroacetamide type protein protection agent, 1-10mg / mL preservative, 5-10mg / mL polyol compound and 1-5mg / mL buffer solutionof surfactant, wherein the polyol compound is polyol or polysaccharide, and the surfactant is at least one of Brij35, TX-100 and peregal O-20. The magnetic particle reagent can disperse the magnetic particles uniformly and stably for a long time and can maintain activity and stability of streptavidin or anti-fluorescein isothiocyanate antibody and protein in unfavorable environment like high temperature, so that chemiluminescence immunoassay is large in chemiluminescence value, high in repeatability and small in background value.

Description

technical field [0001] The invention relates to a magnetic particle reagent and a kit for chemiluminescence immunoassay, belonging to the technical field of chemiluminescence immunoassay. Background technique [0002] In recent years, there have been a variety of immunoassay methods in the field of immunoassay, among which magnetic particle chemiluminescence immunoassay is a new type of labeled immunoassay technology that combines chemiluminescence system with immune reaction to detect trace antigens or antibodies. It has the advantages of good selectivity, high sensitivity, strong specificity, no radioactive hazard, fast analysis speed, simple equipment, etc. It has been widely used in the fields of environment, clinical, food, drug detection, etc. development trend. [0003] At present, magnetic particle chemiluminescence immunoassay kits have been widely used in the market, but the magnetic particle reagents in the existing kits have poor stability in adverse environment...

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Application Information

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IPC IPC(8): G01N21/76G01N33/543
CPCG01N21/76G01N33/54326G01N33/54333G01N2446/84
Inventor 张娜娜徐滕王晓燕周慧林
Owner SUZHOU HYBIOME BIOMEDICAL ENG CO LTD
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