Section fishing device

Abstract

The invention discloses a section fishing device which is composed of a section fishing ring, a section fishing ring handle, a section fishing ring handle clamping groove and a handheld rod, wherein aspot film opening is arranged on the section fishing ring, an arc is arranged at the tail end of the section fishing ring handle, L-shaped lateral walls are arranged on the two sides of the section fishing ring handle clamping groove to fix the arc of the section fishing ring handle, and a smoking and spreading device clamping groove is arranged at the front end of the handheld rod to fix a smoking and spreading device. When the section fishing device is used, the section fishing ring is aligned at a section floating on water, the section fishing device moves downwards to cover the section and contact water to circle the section in the section fishing ring, the section fishing ring with section liquid drops is slightly lifted up to be away from water and cover a copper net, filter paper is utilized to absorb water from the spot film opening on the section ring, the section is attached to the copper net, and the filter paper can slightly press the copper net from the spot film openingto separate the fishing device from the copper net. The section fishing device disclosed by the invention is convenient and effective, overcomes the defects that some samples are difficult to fish, the copper net and the fishing device are difficult to separate in a section fishing process, the section fishing ring cannot be replaced and the like, improves experiment efficiency and saves experiment cost and labor.

Description

technical field [0001] The invention relates to a technique for scooping up samples, in particular to a slice scooping device. The slice picking device is used for transmission electron microscope observation in biomedical research. Background technique [0002] Transmission electron microscopy is an important means of medical biology research, used to observe the ultrastructure of samples. For most biological or material samples, traditional TEM specimen preparation methods use chemical or physical methods to fix, dehydrate and dry, use embedding agents such as epoxy resin for embedding polymerization, use glass knife or diamond knife on ultrathin microtome Ultrathin sections were carried out on the embedding blocks, and the ultrathin sections were taken out onto a grid such as a copper grid, and then double-stained with uranyl acetate and lead citrate. The specific steps are: use glutaraldehyde fixative to soak the sample for pre-fixation; phosphate buffer to rinse the s...

AI Technical Summary

Problems solved by technology

There are the following problems in these methods of slicing: 1. When using tweezers to pick up the copper mesh to take the slice, care must be taken to prevent the tweezers from damaging the copper mesh. Failed; 2. When using tweezers to pick up the copper mesh upside down or fish the slice forward, because the slice floats on the liquid surface exposed to the air, the slight flow of the air flow will cause the slice to move on the water surface, and the slice position cannot be fixed. 3. When using tweezers to pick up the copper mesh upside down or forward to pick up the slices, the static electricity carried by the tweezers or the copper mesh will also make the slices floating on the liquid surface drift and make it difficult to pick up the slices , it is necessary to destaticize the tweezers and copper mesh in advance, which increases the complexity and difficulty of the experimental operation; 4. Using tweezers to pick up the copper mesh upside down or forward to pick up slices is affected by the surface tension of water, and it is difficult to judge what is caught. The number of slices and the position of the slices on the copper grid; 5. After using the tweezers to pick up the copper grid, it is necessary to use filter paper to absorb the liquid on the copper grid so that the slices can be pasted on the copper grid. Slow water absorption and incomplete water absorption can easily cause slice wrinkles , It is also easy to cause improper operation to make the filter paper absorb water and make the slices gather to the edge of the copper mesh, which will affect the experimental results, and even the filter paper will absorb the slices away from the copper mesh while absorbing water, resulting in the failure of picking the slices; 6. Use tweezers to pick up After the copper mesh is buckled upside down or fished in a forward direction, when separating the tweezers and the copper mesh, the copper mesh often adheres to the tweezers because the copper mesh is not completely dry, which increases the difficulty of separation; Thin slice picking can delineate floating slices and use the surface tension of water to lift the slices up. It can avoid the falling off of the clamping copper mesh and reduce the failure rate of slices caused by static electricity and air flow. However, the commonly used metal slicer For example, the surface of copper scooping device is easy to form copper rust in humid air and damage it, and it is also easy to contaminate the slices and affect the experimental results; Copper or stainless steel scoopers are more likely to be corroded and damaged during the scooping process. The chemical properties of platinum are stable, and the scoopers made of it are not easy to be corroded, but the price is expensive, the maintenance cost is high, and there is also a copper mesh after scooping. It is difficult to separate from the scooper; 9. The handles of the commonly used metal scoopers are mostly smooth and cylindrical, and they are easy to roll and slide during use or placement.

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