ORF069 (Open Reading Frame 069) gene deleted strain of infectious spleen and kidney necrosis virus as well as preparation method and application thereof

A technology for spleen and kidney necrosis virus and gene deletion, which is applied in the direction of microorganism-based methods, biochemical equipment and methods, viruses, etc., can solve the problems that have not been reported in the research, and achieve high immunity rate, good immunity effect and cost saving Effect

Active Publication Date: 2018-08-10
SUN YAT SEN UNIV
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  • Abstract
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Problems solved by technology

However, there is no report on the study of ISKNV ORF069 gene deletion strain as gene deletion live attenuated vaccine

Method used

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  • ORF069 (Open Reading Frame 069) gene deleted strain of infectious spleen and kidney necrosis virus as well as preparation method and application thereof
  • ORF069 (Open Reading Frame 069) gene deleted strain of infectious spleen and kidney necrosis virus as well as preparation method and application thereof
  • ORF069 (Open Reading Frame 069) gene deleted strain of infectious spleen and kidney necrosis virus as well as preparation method and application thereof

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Embodiment Construction

[0052] The following description is a preferred embodiment of the present invention, it should be pointed out that for those skilled in the art, without departing from the principle of the present invention, some improvements and modifications can also be made, and these improvements and modifications are also considered Be the protection scope of the present invention.

[0053] The preferred embodiments of the present invention will be described in detail below with reference to the accompanying drawings. The experimental method that does not indicate specific condition in the embodiment, generally according to routine condition. Unless otherwise specified in the examples of the present invention, all reagents and consumables used are commercially available.

[0054] The embodiment of the present invention provides a preparation method of recombinant virus vaccine with ORF069 gene deletion of infectious spleen and kidney necrosis virus, comprising the following steps:

[00...

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Abstract

The invention provides an ORF069 (Open Reading Frame 069) gene deleted strain of an infectious spleen and kidney necrosis virus as well as a preparation method and application thereof. The ORF069 genedeleted strain is an attenuated viral strain prepared after the ORF069 gene is deleted from an infectious spleen and kidney necrosis viral strain. The invention also provides a selection marker carrying ORF069 gene deleted strain of the infectious spleen and kidney necrosis virus as well as a preparation method and application thereof. The selection marker carrying ORF069 gene deleted strain is an attenuated viral strain prepared after the ORF069 gene is deleted from the infectious spleen and kidney necrosis virus and further a selection marker gene is inserted into a gene deleted position through homologous recombination. The ORF069 gene deleted strain of the infectious spleen and kidney necrosis virus, which is provided by the invention, is an ORF069 knocked-out recombinant gene engineering vaccine, is lower in virulence, and is used for greatly reducing the morbidity and lethality of an immunized fish; the obvious immunization effect can be achieved by adopting immersion immunization; the injection is not needed, and the application value is extremely high.

Description

technical field [0001] The invention relates to the field of aquaculture disease prevention and control, in particular to an infectious spleen and kidney necrosis virus ORF069 gene deletion strain and its preparation method and application. Background technique [0002] Mandarin fish, also known as sweet-scented osmanthus fish, has become one of the main freshwater farmed fishes in my country because of its fast growth, good quality and delicious taste. The iridescent virus disease of mandarin fish caused by infectious spleen and kidney necrosis virus (ISKNV) is the main disease in mandarin fish farming, causing the mortality rate of mandarin fish to be close to 100%, which has become the main bottleneck restricting the development of mandarin fish farming. [0003] Infectious spleen-kidney necrosis virus (ISKNV) is a representative species of the genus Dymnovirus, and the group nucleic acid sequence of its whole gene has been determined. ORF), encoding lengths from 40 to 1...

Claims

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Application Information

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IPC IPC(8): C12N7/00C12N7/01C12N15/85A61K39/12A61P31/20C12R1/93
CPCA61K39/12A61P31/20C12N7/00C12N15/85C07K14/005A61K2039/5254A61K2039/552C12N2710/00051C12N2710/00034C12N2710/00022C12N2710/00021C12N2800/106
Inventor 何建国郭长军曾若云林易凡翁少萍何键
Owner SUN YAT SEN UNIV
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