A kind of separation and purification method and application of kelp algae inhibitory active substance
A technology of algal inhibitory activity, separation and purification, which is applied in the separation/purification of hydroxyl compounds, chemical instruments and methods, botanical equipment and methods, etc., to achieve the effects of good algae inhibitory activity, high material purity, and reasonable method design.
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Examples
Embodiment 1
[0015] Embodiment 1, the separation and purification method of kelp algae-inhibiting active substance:
[0016] (1) The dry powder of kelp is added with anhydrous methanol, and extracted at 15-20°C for 12 hours to obtain the extract. The extraction was repeated 3 times, and the extracts were combined. After filtering and evaporating to dryness under reduced pressure, the kelp extract was prepared. Add 90% methanol aqueous solution to this extract, shake fully, let it stand overnight at 4°C, centrifuge, filter, remove the precipitate, and keep the supernatant. After taking appropriate amount of this supernatant and evaporating to dryness under reduced pressure, be dissolved in methanol, this methanol solution is carried out anti-algae activity detection, the result shows that it significantly inhibits the growth of Karenia militaris;
[0017] (2) Add the rest of the supernatant to ethyl acetate and extract 3 times. The upper layer was evaporated to dryness under reduced pres...
Embodiment 2
[0019] Embodiment 2, the separation and purification method of kelp algae-inhibiting active substance:
[0020] (1) Dried kelp was crushed into 0.3 mm powder, added with anhydrous methanol, leached at 15-20°C for 12 hours, repeated leached 3 times, and combined the extracts. After filtering and evaporating to dryness under reduced pressure, the kelp extract was prepared. Add 90% methanol aqueous solution to this extract, shake fully, let it stand overnight at 4°C, centrifuge, filter, remove the precipitate, and keep the supernatant. Take an appropriate amount of the supernatant, evaporate to dryness under reduced pressure at 60°C, dissolve it in methanol, and carry out the detection of algae-inhibiting activity (in the present invention, unless otherwise specified, the detection method of algae-inhibiting activity is the same). On a 96-well plate, 1 μL of the component solution to be tested was added to 199 μL of algae liquid, and 1 μL of anhydrous methanol was added to 199 μ...
Embodiment 3
[0024] Embodiment 3, the separation and purification method of kelp algae-inhibiting active substance:
[0025]Weigh 1000 g of dry kelp powder, add 3 L of anhydrous methanol, extract at 15-20 ° C for 12 hours, and pour out the extract. Add fresh anhydrous methanol again, and extract under the same conditions as above. After repeating 2 times, the extracts were combined (3 times in total). After filtering and evaporating to dryness under reduced pressure, 30.16 g of kelp extract was prepared. Add 150 mL of 90% methanol aqueous solution to this extract, shake fully, let stand overnight at 4°C, centrifuge, filter, remove precipitate, and obtain 130 mL of supernatant.
[0026] Take 1 mL of the supernatant, evaporate to dryness under reduced pressure at 60 °C, dissolve in 20 mL of methanol, and prepare a 10 g / L mother solution for the detection of algae inhibitory activity. On a 96-well plate, 1 μL of the component solution to be tested was added to 199 μL of algae liquid, and 1...
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More