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Preparation method, product and application of molecularly imprinted polymer

A technology of molecular imprinting and polymers, applied in the field of biomedicine, can solve problems such as toxic and side effects

Active Publication Date: 2018-08-17
刘飞
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, chemotherapeutic drugs are the most widely used, such as doxorubicin, cisplatin, paclitaxel, etc. in clinical use. However, these chemotherapeutic drugs can not only kill cancer cells, but also have greater toxicity to normal cells, so in the course of medication have adverse side effects

Method used

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  • Preparation method, product and application of molecularly imprinted polymer
  • Preparation method, product and application of molecularly imprinted polymer
  • Preparation method, product and application of molecularly imprinted polymer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Embodiment 1: preparation polymer NIP-1

[0038] Accurately weigh methacrylic acid (32.0μL), N-isopropylacrylamide (73.2mg), N,N-dimethylbisacrylamide (4.2mg), ultrasonically dissolve in 80ml of water; then add N-tert-butyl Acrylamide (31.1mg) in absolute ethanol (1ml) solution, ultrasonically degassed for 10min; adjust pH=5.8~6.2; slowly add initiator (ammonium persulfate (60.0mg), sodium bisulfite (20.0mg ), sodium lauryl sulfate (40.0mg) dissolved in 20mL of distilled water), polymerized at 33-36°C for 20-26h; dialyzed the crude polymer to remove unpolymerized initiator or / and Methacrylic acid or / and N-isopropylacrylamide or / and N-tert-butylacrylamide to obtain polymer NIP-1.

Embodiment 2

[0039] Embodiment 2: preparation polymer NIP-2

[0040] Accurately weigh methacrylic acid (27.0μL), N-isopropylacrylamide (73.9mg), N,N-dimethylbisacrylamide (3.9mg), ultrasonically dissolve in 80ml of water; then add N-tert-butyl Acrylamide (39.2mg) in absolute ethanol (1ml) solution, ultrasonically degassed for 10min; adjust pH=5.8~6.2; slowly add initiator (ammonium persulfate (60.0mg), sodium bisulfite (20.0mg ), sodium lauryl sulfate (40.0mg) dissolved in 20mL of distilled water), polymerized at 33-36°C for 20-26h; dialyzed the crude polymer to remove unpolymerized initiator or / and Methacrylic acid or / and N-isopropylacrylamide or / and N-tert-butylacrylamide to obtain polymer NIP-2.

Embodiment 3

[0041] Embodiment 3: preparation polymer NIP-3

[0042] Accurately weigh methacrylic acid (21.0μL), N-isopropylacrylamide (73.9mg), N,N-dimethylbisacrylamide (3.8mg), ultrasonically dissolve in 80ml of water; then add N-tert-butyl Acrylamide (47.5mg) in absolute ethanol (1ml) solution, degassed by ultrasonic for 10min; adjust pH=5.8~6.2; slowly add initiator (ammonium persulfate (60.0mg), sodium bisulfite (20.0mg ), sodium lauryl sulfate (40.0mg) dissolved in 20mL of distilled water), polymerized at 33-36°C for 20-26h; dialyzed the crude polymer to remove unpolymerized initiator or / and Methacrylic acid or / and N-isopropylacrylamide or / and N-tert-butylacrylamide to obtain polymer NIP-3.

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Abstract

The invention provides a preparation method, a product and an application of a molecularly imprinted polymer. The preparation method of the molecularly imprinted polymer mainly includes the steps: performing interaction on yfqsmdk-C10 short peptides serving as template molecules with methacrylic acid, N-isopropylacrylamide, N, N-dimethyl bisacrylamide and N-n-tert-butyl acrylamide by non-covalentbonds (such as hydrogen bonds and Van der Waals force); adding an appropriate quantity of initiators to perform polymerization reaction, and imprinting the surface of the polymer with short peptide molecules; eluting the short peptide molecules imprinted on the surface by proper solvents (namely, extraction agents) after polymerization reaction to reserve a cavity capable of specifically recognizing the short peptide molecules. As the short peptide molecules are highly expressed on the surface of a tumor, after the short peptides are eluted, the formed cavity can specifically recognize the short peptide molecules highly expressed on the surface of the tumor, and selectivity and targeting ability of the molecularly imprinted polymer for tumor cells are finally realized.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to a preparation method of a molecularly imprinted polymer, and also relates to a molecularly imprinted polymer prepared by the preparation method, and the use of the molecularly imprinted polymer in the preparation of tumor-targeting drug carriers Applications. Background technique [0002] Cancer is an important risk factor for human life and health. In recent years, the morbidity and mortality of cancer have been increasing. The current treatment methods for cancer mainly include chemotherapy, radiotherapy and other methods. Among them, chemotherapeutic drugs are the most widely used, such as doxorubicin, cisplatin, paclitaxel, etc. in clinical use. However, these chemotherapeutic drugs can not only kill cancer cells, but also have greater toxicity to normal cells, so in the course of medication Unfavorable toxic side effects will occur. Therefore, how to reduc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08F222/38C08F220/54C08F220/06C08J9/26B01J20/26B01J20/30A61K47/32A61P35/00
CPCA61K47/32A61P35/00B01J20/261B01J2220/4812C08F222/385C08J9/26C08J2201/0424C08J2335/00C08F220/54C08F220/06
Inventor 刘云啸
Owner 刘飞
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