InDel molecular marker related to cashmere traits of goat and application of InDel molecular marker

A molecular marker, goat technology, applied in the field of molecular biology, to achieve the effect of accurate and reliable method, promoting the breeding process and easy operation

Inactive Publication Date: 2018-08-17
INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For a long time, the molecular markers related

Method used

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  • InDel molecular marker related to cashmere traits of goat and application of InDel molecular marker
  • InDel molecular marker related to cashmere traits of goat and application of InDel molecular marker
  • InDel molecular marker related to cashmere traits of goat and application of InDel molecular marker

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Example 1 Genome-wide selection signal analysis of cashmere-producing goats and non-cashmere-producing goats

[0042] The whole-genome resequencing data of 66 non-cashmere goats in Central Pakistan, Nepal, and China and 51 cashmere-producing goats from China were analyzed for the gene-based selection signal of cashmere-non-cashmere production, and it was found that it is located on chromosome 6 of goats. The FGF5 gene locus was most closely related to cashmere-non-downy traits. Through further data mining, it was found that the InDel molecular marker located at 95454685-95455189 bp on chromosome 6 of goats was most closely related to cashmere traits. The polymorphism of the InDel molecular marker is - / insertion sequence, and the nucleotide sequence of the insertion sequence is shown in SEQ ID NO.1.

Embodiment 2

[0043] Example 2 Identification of the InDel polymorphic site of the Chinese goat FGF5 locus

[0044] 1. Extract the genomic DNA of Pakistani, Nepalese and Chinese goat breeds to be tested

[0045] Among them, there were 146 non-cashmere goats (including 27 non-cashmere goats from Pakistan, 7 Xiangdong black goats from Hunan, 17 Hainan black goats from Hainan, 25 goats from the plains of Nepal, and 7 goats from Shangrao, Jiangxi. 34 Guangfeng goats, 7 Laoshan dairy goats, 10 Tugenbao dairy goats, 19 Yunling black goats); a total of 153 cashmere goats (including 10 Bangor cashmere goats from Tibet, Ritu 10 cashmere goats; 25 Arbas cashmere goats from Inner Mongolia, 4 Alashan cashmere goats, 10 Han cashmere goats; 25 Liaoning cashmere goats from Liaoning; 18 Jining green goats from Shandong, from 30 Southern Xinjiang cashmere goats in southern Xinjiang; 21 cashmere goats from high-altitude areas in Nepal), using conventional methods to extract genomic DNA from blood.

[0046] 2...

Embodiment 3

[0051] Example 3 Expanded population analysis of the polymorphic site of the FGF5 gene locus of 299 goats

[0052] According to Example 1, the genomic DNA samples of 153 cashmere-producing goats and 146 non-cashmere-producing goats were extracted, and the specific primers in Example 1 were used for PCR detection, followed by agarose gel electrophoresis, and genotyping based on Sanger sequencing detection. The 153 cashmere goats are Tibetan Bangor cashmere goats, Tibet Ritu cashmere goats, Inner Mongolia Alashan cashmere goats, Inner Mongolia Arbas cashmere goats, Inner Mongolia Han cashmere goats, Liaoning cashmere goats, Nanjiang cashmere goats, Jining green goats 1, Nepal high-altitude goats; 146 non-cashmere goats, namely Pakistan goats, goats in the plains of Nepal, Yunling black goats, Hainan black goats, Guangfeng goats, Hunan black goats, Laoshan milk goats, Tugenbao milk goats goat. Table 1 shows the genotyping results of the FGF5 locus of the FGF5 gene sequence show...

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Abstract

The invention relates to an InDel molecular marker related to cashmere traits of goat and an application of the InDel molecular marker. The marker is located between the 95454685bp and the 95455189bpof the No.6 chromosome of the goat; the polymorphism of the InDel molecular marker is -/insertion fragment; and a sequence of the insertion fragment is shown as SEQ ID NO.1. The invention also provides specific primers and a method for detecting the InDel marker; and sequences of the specific primer pair are shown as SEQ ID NO.2-3. Through the specific primer pair provided by the invention, on thebasis of an electrophoretic band obtained through PCR and agarose gel electrophoresis as well as analysis and statistics on Sanger sequencing and in accordance with cashmere goat allele and non-cashmere goat allele types on an InDel site, it can identify cashmere or non-cashmere traits of a goat variety. The InDel molecular marker provided by the invention is low in amount of detected samples inneed, simple and convenient in method and accurate in identifying result, and the InDel molecular marker has a good prospect of application and promotion.

Description

technical field [0001] The invention relates to the technical field of molecular biology, in particular to an InDel molecular marker related to cashmere-producing traits of Chinese goats and a method for detecting cashmere-producing goats and non-cashmere-producing goats. Background technique [0002] Insertion-deletion (InDel) polymorphism refers to a special type of biallelic genetic marker in the genome, which is characterized by the insertion or deletion of DNA fragments of different sizes in the genome, and has the advantages of both STR and SNP. InDel detection methods, currently commonly used, include agarose gel electrophoresis, sanger sequencing, and the latest high-throughput next-generation sequencing technologies. Detection of genotype by detecting InDel polymorphism is a method that has emerged in recent years. As genetic markers, InDel molecular markers have been widely used in research fields such as forensic identification, genetic breeding and genetic diver...

Claims

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Application Information

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IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/156
Inventor 蒋琳宋伸李业芳浦亚斌刘雪雪张艳丽马月辉
Owner INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
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