Microfluidic detection chip, kit and whole blood multi-index detection method based on same and application of microfluidic detection chip

A detection chip and microfluidic technology, applied in the field of medical detection, can solve problems such as chip function failure, unfavorable on-site detection, complex equipment, etc., and achieve the effect of convenient and flexible use and stable and reliable quantitative detection results

Active Publication Date: 2018-08-24
SHANGHAI IGENETEC DIAGNOSTICS CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in this method, blood cells are separated by centrifugation. During the detection process, multiple centrifuges are required to transport the sample to a designated location. The equipment is relatively complicated, which is not conducive to on-site detection.
In addition, during the use of this chip, there is a risk that the diluent will enter the diluent quantitative tank during the sample addition process and enter the mixing tank in advance. This improper operation will cause the chip to fail.

Method used

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  • Microfluidic detection chip, kit and whole blood multi-index detection method based on same and application of microfluidic detection chip
  • Microfluidic detection chip, kit and whole blood multi-index detection method based on same and application of microfluidic detection chip
  • Microfluidic detection chip, kit and whole blood multi-index detection method based on same and application of microfluidic detection chip

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0077] This embodiment provides a microfluidic detection chip for detecting blood lipid content in whole blood, including cholesterol, triglycerides, high-density lipoprotein cholesterol, and low-density lipoprotein cholesterol.

[0078] figure 1 It is a split diagram of the microfluidic detection chip. As shown in the figure, the microfluidic detection chip 1 includes an upper layer 10 of the chip, that is, a sample processing layer, and a lower layer 20 of the chip, that is, a reaction layer. figure 2 It is a schematic cross-sectional view of the microfluidic detection chip along the diagonal direction. It can be seen from the figure that the two layers are closely overlapped to form a closed fluid channel system inside the chip. The chip material is PMMA. The size of the chip is 30mm×30mm×5mm.

[0079] image 3 Shown is the upper surface of the chip upper layer 10, that is, the sample processing layer. It can be seen that the sample processing layer includes one sample hole 10...

Embodiment 2

[0093] This embodiment provides a microfluidic chip for detecting the content of six indicators of liver function in whole blood, specifically including total protein, albumin, total bilirubin, alanine aminotransferase, and aspartate amino transfer Enzymes and cholinesterase.

[0094] The basic structure is similar to the first embodiment, and the upper and lower layers of the chip are shown in Figure 7 with Figure 8 . The size of the chip is 30mm×30mm×5mm. The chip material is PC. by Figure 7 It can be seen that the microfluidic detection chip has 6 fluid channels 104 corresponding to 6 vent holes 103 and 6 detection holes 106. The fluid channels 104 are evenly distributed around the sample adding area 101, and the sample adding area 101 of the sample processing layer A cut whole blood filter membrane is fixed at the same place as in Example 1. The length of the fluid channel is 7.4mm, the width is 1.2mm, and the depth is 2mm. A connecting channel 105 is provided on the s...

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Abstract

The invention provides a microfluidic detection chip, a kit and a whole blood multi-index detection method based on the same and an application of the microfluidic detection chip. The detection chip comprises two layers, the upper layer is a sample processing layer and the lower layer is a reaction layer, two or more fluid channels are arranged on the surface, facing the reaction layer, of the sample processing layer, and the two layers are closely overlapped to form a closed fluid channel system inside the chip. A plurality of biochemical indexes based on colorimetric measurement, such as liver function, kidney function, blood fat, electrolyte and the like are detected simultaneously in a small test card with a microfluidic chip technology. Difficulties in the application of the existingmicrofluidic technology are overcome, and the chip has low requirements for detection equipment, can be easily applied on site for POCT (point-of-care testing), and is applicable to large, medium andsmall medical institutions, emergency departments, community hospitals, family detection, scientific research and the like.

Description

Technical field [0001] The invention belongs to the field of medical detection, and in particular relates to a whole blood detection microfluidic chip and a detection system using it. Background technique [0002] As we all know, blood is closely related to the tissues and organs of various systems throughout the body. Blood testing is the most extensive sample testing in biomedicine, clinical diagnosis, and health quarantine diagnosis. At present, most hospitals and laboratories use large centrifuges to separate serum and blood cells, and then use automated biochemical analyzer equipment to detect the content of various blood indicators. However, using this method requires a blood sample of milliliters to be drawn from the patient, and the actual sample used for testing is only a few microliters, resulting in a large amount of waste of samples, and the centrifuge is large in size and complex in structure, and cannot be directly connected to blood testing equipment. As a result,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01L3/00G01N33/48
CPCB01L3/5027B01L2200/10B01L2300/0861B01L2300/0887B01L2300/12G01N33/48
Inventor 方雪恩吴静孔继烈
Owner SHANGHAI IGENETEC DIAGNOSTICS CO LTD
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