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Method for detecting iron content in water sample by using methyl-hydroxamic acid spectrophotometric method

A technology of methylhydroxime and iron content, applied in the measurement of color/spectral characteristics, etc., can solve the problem of low selectivity, and achieve the effect of good selectivity and easy operation

Inactive Publication Date: 2018-08-28
XINYANG NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

These reagents for direct color reaction with iron mainly include o-phenanthroline, sulfosalicylic acid, etc., which have the advantage of high sensitivity, but the disadvantage is that the selectivity is not high

Method used

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  • Method for detecting iron content in water sample by using methyl-hydroxamic acid spectrophotometric method
  • Method for detecting iron content in water sample by using methyl-hydroxamic acid spectrophotometric method

Examples

Experimental program
Comparison scheme
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Embodiment 1

[0025] A methyl hydroxamic acid photometric method for detecting iron content in a water sample includes the following steps:

[0026] 1) Fe 3+ Preparation of standard solution: add Fe 3+ The stock solution is diluted with distilled water to 10.0μg / mL Fe 3+ standard solution;

[0027] 2) Determination of absorbance: Pipette 0.02 ml, 0.40 ml, 0.80 ml, 1.20 ml, 1.60 ml, 2.00 ml, 2.40 ml of 10.0μg / mL Fe respectively 3+ Standard solution in a 25ml volumetric flask, add 2.0ml of 0.207mol / L methyl hydroxamic acid solution, 1ml of 1+1 hydrochloric acid, 5.0ml of aminoacetic acid-hydrochloric acid buffer with pH 2.3, 5.0ml of 3% H 2 O 2 , Shake well, dilute to 25ml with distilled water, take a 1cm cuvette, and measure the corresponding absorbance at the maximum absorption wavelength of 480nm with the reagent blank as a reference;

[0028] 3) Draw the standard curve: draw the standard curve with iron content as the abscissa and the corresponding absorbance as the ordinate, and use one-variabl...

Embodiment 2

[0031] Example 2 Test analysis

[0032] 2.1 Absorption curve

[0033] Take 2.0 ml Fe 3+ Standard solution, refer to the experimental method in Example 1, measure the absorbance every 10nm between 400 and 600nm, draw the absorption curve, see figure 1 Shown. Of which: Series 1-Fe 3+ +Methyl hydroxamic acid system, series 2-Fe 3+ +Methyl Hydroxamic Acid+H 2 O 2 system.

[0034] by figure 1 It can be seen that 3.0% H 2 O 2 Join Fe 3+ -After methyl hydroxamic acid system, its maximum absorption wavelength is still at 480nm, no red shift occurs, but its absorbance value increases, thus improving the sensitivity of the color reaction, and H 2 O 2 The addition of Chromium greatly increases the stability of the color-developing system, which can make the color-developing system stand for about a week without fading.

[0035] The influence of acidity on color reaction

[0036] Take 2.0 ml Fe 3+ Standard solution, the method refers to Example 1, adjust the pH value of the buffer solution to f...

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Abstract

The invention provides a method for detecting iron content in a water sample by using a methyl-hydroxamic acid spectrophotometric method. The method comprises the following steps of 1) Fe standard solution preparation; 2) absorbance measurement, wherein 1.5-2.5 milliliters of methyl-hydroxamic acid solution with concentration of 0.207 mole per liter, 1 milliliter of 1+1hydrochloric acid, 5.0 milliliters of a buffer solution with a pH value of 1.5-2.5 and 3.0-5.0 milliliters of 3% of H202 are respectively added into a plurality of Fe standard solutions with different volume amounts and then shaken well, constant volume of 25 milliliters is carried out with distilled water, 1 centimeter of cuvette is adopted, the absorbance is measured by taking reagent blank as reference at the position ofa maximum absorption wavelength; 3) standard curve drawing, wherein the iron content serves as the abscissa, the corresponding absorbance serves as the ordinate, the standard curve is drawn, and a regression equation of the standard curve is calculated; and 4) to-be-measured Fe solution measurement, wherein the absorbance of a to-be-measured Fe<3+> solution is measured, and the iron content of theto-be-measured Fe solution is obtained according to the absorbance. The method for detecting the iron content in the water sample by using the methyl-hydroxamic acid spectrophotometric method has theadvantages that the chromogenic reaction and application of the methyl-hydroxamic acid and iron are studied, the method is applied to the measurement of the iron in the water sample, the recovery rate is 95.5-97.8%, the selectivity is good, and the operation is simple and convenient.

Description

Technical field [0001] The invention relates to a method for detecting iron content in water samples, in particular to a method for detecting iron content in water samples by methyl hydroxamic acid photometry. Background technique [0002] Iron is an important industrial raw material. It is widely used in metallurgy, geology, environment, chemical industry, material science and other fields because of its excellent mechanical properties and good plasticity. Iron is also one of the most important trace elements in the human body. It is the main component of hemoglobin and myoglobin. It is widely involved in the metabolic activities of various enzymes and plays an important role in the body's metabolic process. When the iron content in the human body is insufficient, it may lead to a decline in human immunity and susceptibility to infectious diseases. When there is excess iron in the human body, it will also cause harm to the human body due to lack of excretion channels, and there...

Claims

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Application Information

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IPC IPC(8): G01N21/31
CPCG01N21/31
Inventor 吕艳阳张秀兰
Owner XINYANG NORMAL UNIVERSITY
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