Culture method for halimasch

Abstract

The invention discloses a culture method for halimasch. The method comprises the following steps that 1, strain breeding is conduced, wherein a halimasch strain female parent is screened, separated and purified to select a female strain; 2, strain propagation expanding is conducted, wherein the female strain is placed in a culture vessel, a liquid culture medium is used for culturing a seed liquid-state strain, and the seed liquid-state strain is inoculated to a fermentation tank filled with the liquid culture medium and continues to be cultured to form a halimasch liquid strain; 3, the halimasch liquid strain is transplanted to a solid culture medium and cultured into a culture strain. According to the culture method for halimasch, artificially-bred and clustered rhizomorph with proper thickness, robust growth and white top is selected and separated for strain selection and culture, the quality of a strain source is guaranteed from the source at first, rhizomorph after strain selection also needs to be purified and screened, in this way, rhizomorph can be prevented from being contaminated other hyphae, the purity of the strain after original strain breeding and propagation expanding is 100%, the strain does not contain any other bacteria, and accordingly it can be guaranteed that hyphae bred by the culture strain at last are healthy, robust and pure in variety.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for culturing Armillaria gastrodia elata. Background technique [0002] Armillaria belongs to the order Agaric, Armillaria, and the fruiting bodies are generally medium-sized. There are about 40 species of fungi in this genus in the world, and they are also widely distributed in central China, southwest China and northeast China. Armillaria is an important symbiotic bacteria of the precious traditional Chinese medicine Gastrodia elata. Gastrodia elata cannot absorb nutrients by itself. During its growth period, it mainly uses Armillaria to provide nutrients in the form of rhizobial cords. Therefore, the quality of the strains and the purity of the strains will have a great impact on the yield of Gastrodia elata. In the existing commercial production of Gastrodia elata, the production steps are generally first inoculated with Armillaria sp. on a solid medium to develop and gr...

AI Technical Summary

Problems solved by technology

In this process, most of the attention is paid to the aseptic operation of the culture medium and the operation process, but the inoculated strains themselves are often ignored. The growth conditions of the directly inoculated strains are first of all different, and it is impossible to guarantee that they are all high-quality strains. Secondly, there may be other miscellaneous bacteria mixed in it, but it is difficult to identify it in the state of the strain, unless it can be distinguished after it matures, directly using the strain to inoculate not only cannot guarantee the purity of the strain, but also The quality of the cultivars is also uneven. The cultivation of such strains into cultivars will not only affect the quality and yield of the cultivars, but also lead to a high rate of empty ponds after being associated with Gastrodia elata in the later stage.