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Water-soluble fullerene nano-material and preparation method and applications thereof

A technology of nanomaterials and fullerenes, applied in the field of water-soluble fullerene nanomaterials and its preparation method and application, to achieve high tumor inhibition rate, good affinity, and the effect of being conducive to popularization and application

Active Publication Date: 2018-09-04
BEIJING FUNAKANG BIOTECH CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, one of the difficulties in the widespread application of embedded metallofullerenes is how to synthesize and separate them in large quantities.

Method used

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  • Water-soluble fullerene nano-material and preparation method and applications thereof
  • Water-soluble fullerene nano-material and preparation method and applications thereof
  • Water-soluble fullerene nano-material and preparation method and applications thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] Embodiment 1, C 70 (OH) x (Amino Acid) y Synthesis

[0053] (1) Fullerene C 70 (OH) 29 Synthesis

[0054] C in toluene solution 70 Mix with a NaOH solution with a concentration of 50%, add a small amount of phase transfer catalyst tetramethylammonium hydroxide (TBAH), stir for 24 hours, add ethanol to precipitate the product, and dialyze to remove excess NaOH to obtain the product C 70 (OH) 29 , freeze-dried and stored.

[0055] (2)C 70 (OH) 13 (CH 2 OHCHNHCOOH) 4 Synthesis

[0056] The alkali solution of NaOH and serine (wherein the mass fraction of NaOH is 33%, the molar ratio of serine and NaOH is 1:4) is mixed with ethanol, and added dropwise to C 70 (serine and C 70 In a toluene solution with a molar ratio of 100:1), stir overnight (12h), remove the upper toluene layer, and dialyze the lower layer to remove excess NaOH, freeze-dry and store.

Embodiment 2

[0057] Embodiment 2, C 70 (OH) x (Amino Acid) y Number of modified hydroxyl groups and amino acids

[0058] (1) Determination of C 70 (OH) 29 hydroxyl number

[0059] According to elemental analysis (Flash EA 1112) result: C 53.55%, H 3.19%, Nfigure 1 shown) to calculate C 70 (OH) x number of hydroxyl groups. From thermogravimetric analysis, it can be known that C 70 (OH) x The solid powder contains 11.8% water, which is about 10 water molecules. Combined with the H content in the elemental analysis, it is estimated that the H content in the hydroxyl group is 1.87%. From this, the C can be calculated. 70 (OH) x The number of hydroxyl groups is 29.

[0060] (2) Determination of C 70 (OH) 13 (CH 2 OHCHNHCOOH) 4 The number of hydroxyl and serine

[0061] According to elemental analysis (Flash EA 1112) result: C 47.99%, H 3.21%, N 2.68%, combined thermogravimetric analysis, derivative thermogravimetric analysis curve (such as figure 2 shown) to calculate C 70 (O...

Embodiment 3

[0062] Embodiment 3, the mensuration of the hydrated particle size of water-soluble fullerene nanomaterial

[0063] Get the C prepared by the embodiment of the present invention 1 in a small amount 70 (OH) 29 and C 70 (OH) 13 (CH 2 OHCHNHCOOH) 4 The powder is dissolved in water to form a dilute solution. Due to the intermolecular interaction, C 70 (OH) 29 and C 70 (OH) 13 (CH 2 OHCHNHCOOH) 4 Agglomerates into nanoparticles in water to form particles with a hydrated particle size of 1-200nm.

[0064] The hydrated particle size of the nanoparticles in pure water at pH=7.0 was measured by dynamic light scattering (DLS, Zetasizer Nano ZSP), C 70 (OH) 29 and C70 (OH) 13 (CH 2 OHCHNHCOOH) 4 The average particle diameters are 136.4±0.5nm and 134.7±0.4nm respectively, see image 3 .

[0065] A high-resolution atomic force microscope ( 4BioScience AFM) measured C 70 (OH) 29 and C 70 (OH) 13 (CH 2 OHCHNHCOOH) 4 The sizes of nanoparticles aggregated in water are ...

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Abstract

The invention discloses a water-soluble fullerene nano-material and a preparation method and applications thereof. The general structural formula of the water-soluble fullerene nano-material is C<2n>(OH)<x>(Amino Acid)<y>, wherein 20 <= n <= 60, 0 <= x < 50, 0 <= y < 20, and the Amino Acid is a water-soluble amino acid. The water-soluble fullerene nano-material can be applied to prepare medicinesof tumour treatment, the tumour is at least one kind of the following diseases: liver cancer, lung cancer, colorectal cancer, kidney cancer, pancreatic cancer, osteocarcinoma, mammary cancer, ovariancancer, prostatic cancer, esophageal cancer, gastric cancer, oral cancer, nasal cancer, laryngocarcinoma, cholangiocarcinoma, cervical carcinoma, uterine cancer, testicular cancer, meningioma, skin cancer, melanoma and sarcoma. The material has good water solubility and good endophilicity with organisms, and can obviously block tumor vessels and rapidly cut off nutritional supply of tumor tissues;and the material is low in preparation costs.

Description

[0001] The present invention requires Beijing Funakang Biotechnology Co., Ltd. to submit to the Chinese Patent Office on October 8, 2016, the application number is CN201610878746.1, and the title of the invention is "a water-soluble fullerene nanomaterial and its preparation method and application." The priority of the Chinese patent application ", the entire content of which is incorporated in the present invention by reference. technical field [0002] The invention relates to a water-soluble fullerene nano material and a preparation method and application thereof, belonging to the field of biomedicine. Background technique [0003] Cancer, also known as malignant tumor, is the abnormal proliferation and metastasis of cells caused by the loss of normal gene regulation of cells in local tissues under the stimulation of various carcinogenic factors. According to the "World Cancer Report 2014" published by the International Agency for Research on Cancer under the World Health...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K33/44A61K47/54A61K47/64A61P35/00A61N5/00
CPCA61K33/44A61N5/00A61N5/0601A61N5/0613A61N2005/0602A61N2005/0664
Inventor 王春儒周悦甄明明白春礼
Owner BEIJING FUNAKANG BIOTECH CO LTD
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