Application of water-soluble fullerene nanoparticles in preparing medicine for inhibiting tumor growth

A nanoparticle, water-soluble technology that can be used in the field of biomedicine to solve problems such as side effects, normal cell death, and lack of targeting of chemotherapy drugs

Inactive Publication Date: 2018-11-23
BEIJING FUNAKANG BIOTECH CO LTD +1
View PDF9 Cites 7 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the current process of tumor treatment, chemotherapy is one of the most commonly used methods, but chemotherapy drugs lack targeting, and can cause the death of normal cells while killing tumor cells, so it often causes serious side effects

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of water-soluble fullerene nanoparticles in preparing medicine for inhibiting tumor growth
  • Application of water-soluble fullerene nanoparticles in preparing medicine for inhibiting tumor growth
  • Application of water-soluble fullerene nanoparticles in preparing medicine for inhibiting tumor growth

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] Embodiment 1, C 70 Synthesis of -OH

[0058] (a) Add 7mL of 30% mass percent hydrogen peroxide (analytical grade, purchased from Sinopharm Reagent) aqueous solution and 3mL of 40% mass percent sodium hydroxide (analytical grade, purchased from Sinopharm Reagent) solution into a 100mL circle Bottom flask, add 200mg fullerene C 70 Solid (purity: 99%, Xiamen Funa New Material Technology Co., Ltd.), then add a magnetic stirrer (model: B200), use a magnetic stirrer to stir for 24h (temperature: 50 ° C, speed: 1000r / min), use a solvent to filter filter (volume: 1L, filter membrane pore size: 200nm, Jinteng Company) to obtain a brown-yellow solution.

[0059] (b) Add the solution obtained in step (a) into a 50ml centrifuge tube, and then add an excess concentration of 95% ethanol (analytical grade, Sinopharm reagent). After centrifugation (rotational speed: 10000r / min, time: 4min), the colorless solution in the upper layer was removed, and the collected precipitate was diss...

Embodiment 2

[0062] Embodiment 2, water-soluble C 70 Determination of the number of hydroxyl groups in -OH nanoparticles

[0063] For the C prepared in the above-mentioned embodiment 1 70 -OH nanoparticles were analyzed for elements (Flash EA 1112), and combined with thermogravimetric and derivative thermogravimetric results to analyze the number of hydroxyl groups attached to them. From the result of elemental analysis (as shown in the table below), it can be seen that the C 70 In -OH, the C content is 37.85%, the H content is 1.51%, and the N content is <0.3%.

[0064] From thermogravimetric analysis ( figure 2 ) shows that C 70 The -OH solid powder contains 3.7% water, about 5 water molecules, combined with the ratio of H content to C content in elemental analysis, it can be estimated that 24 hydroxyl groups are modified on the surface of the carbon cage. so C 70 The average structural formula of -OH is C 70 (OH) 24 ·5H 2 O.

[0065]

Embodiment 3

[0066] Embodiment 3, water-soluble C 70 Determination of Hydrated Particle Size of -OH Nanoparticles

[0067] Get a small amount of C prepared by Example 1 of the present invention 70 -OH powder is dissolved in water to form a dilute solution, due to intermolecular interactions, C 70 -OH aggregates into nanoparticles in water to form particles with an average hydrated particle size of 1-200nm.

[0068] The hydrated particle size of the nanoparticles in pure water at pH=7.0 was measured by dynamic light scattering (DLS, Zetasizer Nano ZSP), C 70 The average hydrated particle size of -OH is 135.9nm, and its particle size distribution curve can be found in image 3 .

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses an application of water-soluble fullerene nanoparticles in preparing medicine for inhibiting tumor growth. The water-soluble fullerene nanoparticles comprise at least one of water-soluble hollow fullerene nanoparticles, pharmaceutically acceptable salts of the water-soluble hollow fullerene nanoparticles, pharmaceutically acceptable esters of the water-soluble hollow fullerene nanoparticles, water-soluble metal fullerene nanoparticles, pharmaceutically acceptable salts of the water-soluble metal fullerene nanoparticles, and pharmaceutically acceptable esters of the water-soluble metal fullerene nanoparticles. The drug has good affinity to organisms, low toxicity and high inhibition efficiency to on tumors.

Description

[0001] The present invention requires Beijing Funakang Biotechnology Co., Ltd. and the Institute of Chemistry, Chinese Academy of Sciences to submit to the Chinese Patent Office on May 9, 2017, the application number is CN201710322042.0, and the invention name is "Water-soluble fullerene nanoparticles in the preparation of The priority of the Chinese patent application "Applications in Inhibiting Tumor Growth Drugs", the entire content of which is incorporated in the present invention by reference. technical field [0002] The invention relates to the field of biomedicine, in particular to the application of water-soluble fullerene nanoparticles in the preparation of drugs for inhibiting tumor growth. Background technique [0003] Cancer, that is, malignant tumors, as a major disease affecting human life and health in the 21st century, has attracted widespread attention. According to the "World Cancer Report" published by the International Agency for Research on Cancer in 20...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): A61K33/44A61P35/00
CPCA61K33/44
Inventor 王春儒甄明明周悦白春礼
Owner BEIJING FUNAKANG BIOTECH CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap