Mortar used for tissue sample grinding

Abstract

The invention belongs to the field of biotechnology devices, and particularly relates to a mortar used for plant or animal tissues. The mortar is composed of a mortar body and a grinding rod. The mortar body is composed of a grinding pan and a liquid nitrogen pan, a ceramic layer (3) and an insulating layer (4) are arranged on the outer wall and the bottom of the liquid nitrogen pan, and liquid nitrogen is contained into the ceramic layer (3); a rubber layer (8) is arranged on the bottom of the liquid nitrogen pan, and the grinding pan comprises a pan rim (1), a wall support (5), a bottom support (6) and a bottom supporting point (10); a zigzag rim (2) is arranged on the inner wall of the upper portion of the grinding pan, and the zigzag rim (2) is bent inwards; a vibration point (11) is arranged in the center of the bottom in the grinding pan, a round grinding position (9) is arranged around the vibration point (11), and the surface of the grinding position (9) is frosted; the grinding rod is made of a ceramic material, and the grinding rod comprises a handle (12), a limiting ring (13) and a grinding head (14). The mortar has the advantages that less labor is needed during grinding, the pollution caused when a sample is dispersed outwards is prevented, and the liquid nitrogen is saved.

Description

[0001] The invention belongs to the field of biotechnology equipment, in particular to a mortar for plant or animal tissue. Background technique [0002] Sample grinding is a one-step operation often involved in agriculture and other technical fields, specifically including: agriculture, biotechnology, pharmaceuticals, horticulture, etc. As people pay more and more attention to biotechnology experiments, sample grinding tests are more and more widely used. In the sample grinding test, the grinding of the tissue sample is a frequent and laborious operation for the test personnel. For example, in the large-scale extraction of RNA (ribonucleic acid) or DNA (deoxyribonucleic acid) experiments, it needs to be repeated for each experiment at each time point Perform tissue grinding. Whether the organization can grind samples conveniently, quickly and hygienically is directly related to the speed of the test process and the final test effect. [0003] The materials used in the sample...

AI Technical Summary

Problems solved by technology

[0004] The traditional tissue grinding operation has the following defects: (1) The inner edge of the ordinary mortar is designed to be outward, and it is easy to overflow the mortar during the grinding process, and the edge temperature is low, which is easy to cause sample contamination; (2) The ordinary mortar is mixed with liquid nitrogen. When mixing tissue samples, after adding liquid nitrogen, it is necessary to wait for the liquid nitrogen to evaporate completely before grinding, which is a waste of time and liquid nitrogen; In order to fully grind the tissue, it is necessary to frequently pick up the mortar and knock the experimental bench several times to make the powder fall, so it will waste a lot of test time and labor; (4) The surface of the ordinary grinding rod is smooth, and it needs to be tightly gripped during the grinding process. Only the handle of the grinding rod can prevent the hand from slipping, and the slipping of the hand will easily cause the hand (or glove) to come into contact with the tissue sample, resulting in contamination of the sample

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