A microfluidic chip for cell tissue culture and real-time monitoring and its application method

Abstract

The invention discloses a microfluidic chip for cell tissue culture and real-time monitoring and a method for using the chip. The chip includes a glass substrate layer and a PDMS microfluidic channel layer on the glass substrate layer. The glass substrate layer includes a glass substrate and a glass substrate. Several pairs of microelectrodes arranged on the glass substrate layer, the PDMS microfluidic channel layer includes several independent microfluidic channels, and the microelectrode pairs on the glass substrate correspond to the microfluidic channels on the PDMS microfluidic channel layer; The microelectrodes are electrically connected to external circuits. The methods of use include cell capture, cell tissue culture, electrical impedance spectroscopy detection, and tissue release. The chip of the present invention is processed with a transparent base material, and the microscopic imaging can be used as a supplementary means for electrical impedance spectroscopy detection to observe and analyze biological processes such as dynamic growth and physiological behavior of cell tissues in situ. It can be used in fields such as biological research of cells or tissues and drug screening.

Description

technical field [0001] The invention relates to microfluidic technology and a biological cell tissue culture device, in particular to a microfluidic chip for cell tissue culture and real-time monitoring and its application method. Background technique [0002] Before the official application of new drugs, animal experiments and human clinical trials are generally required. Due to the huge difference between animals and humans, the information provided in animal experiments is unreliable, and animal experiments involve ethical issues, while human clinical trials will bring potential harm to the bodies of experimental volunteers due to unstable factors such as side effects of drugs. On the other hand, there are huge uncertainties and risks of failure. Therefore, the dynamic culture of in vitro biological tissues and drug experiments are important means to solve the problems of long drug development cycle and high cost. [0003] The in vitro culture of cells is an important t...

AI Technical Summary

Problems solved by technology

[0006] The development and application of microfluidic tissue chips currently used for tissue culture or drug testing are limited in the following aspects: the lack of effective means to precisely manipulate cells and grow them into three-dimensional spherical tissues; the current real-time Detection is generally achieved through microscopic imaging, and phototoxicity has a certain impact on the physiological state of cells and tissues; it is difficult to perform high-throughput experiments, and the sample data is small, lacking statistical significance; tissue samples are not easy to take out for subsequent research and experiments

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