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Method for biologically preventing, controlling and reducing aphids on wolfberry and improving production performances

A technology of wolfberry aphids and production performance, which is applied in the field of biological control, can solve the problems of increasing the dosage of pesticides, affecting the quality of wolfberry, and increasing pesticide residues, so as to improve the survival rate, improve the production performance of wolfberry, and reduce the effects of wolfberry aphids

Inactive Publication Date: 2018-09-04
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Although pesticides can control the damage of aphids, long-term use will cause aphids to develop drug resistance, resulting in increased dosage of pesticides and increased pesticide residues in wolfberry products, which will affect the quality of wolfberries

Method used

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  • Method for biologically preventing, controlling and reducing aphids on wolfberry and improving production performances
  • Method for biologically preventing, controlling and reducing aphids on wolfberry and improving production performances
  • Method for biologically preventing, controlling and reducing aphids on wolfberry and improving production performances

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] 1) High-density culture of chitinase-producing Sb (Bacillus subtilis CICC 10339), the starting density of Sb is 2.13×10 6 Individuals / mL, inoculated into liquid culture medium (based on 1 L, glucose 11g, soybean powder 20g, shrimp powder 1g, the balance was water, adjusted to pH=6.5, sterilized at 121°C for 20min), the inoculum size was 2%, 36 Cultivate with shaking at ℃ for 22 hours, and the density of the bacterial solution is 3.85×10 9 cfu / mL to obtain a high-density Sb bacterial solution.

[0058] 2) Add corn flour to water and stir to make corn milk. The concentration of corn milk is 50% (that is, the mass ratio of corn flour to water is 50:50). Add α-amylase (EC.3.2.1.1) and glucoamylase (EC. 3.2.1.3) composed of raw amylase (enzyme dosages are 5U / L and 500U / L respectively), cultured with shaking at 37°C for 24hrs, the starch in the corn flour granules was partially hydrolyzed to form holes, dried at 120°C after centrifugation, and became micro Hole cornmeal.

...

Embodiment 2

[0065] 1) High-density culture of chitinase-producing Sb, the starting density of Sb is 1.77×10 6 Individuals / mL, inoculated into liquid culture medium (based on 1 L, glucose 17g, soybean powder 30g, shrimp powder 3g, the balance was water, adjusted to pH=7.5, sterilized at 121°C for 20min), the inoculum size was 4%, 38 Cultivate with shaking at ℃ for 26 hours, and the density of the bacterial solution is 4.12×10 9 cfu / mL to obtain a high-density Sb bacterial solution.

[0066] 2) Add corn flour to water and stir to make corn milk. The concentration of corn milk is 50% (that is, the mass ratio of corn flour to water is 50:50). Add α-amylase (EC.3.2.1.1) and glucoamylase (EC. 3.2.1.3) composed of raw amylase (enzyme dosages are 5U / L and 500U / L respectively), cultured with shaking at 37°C for 24hrs, the starch in the corn flour granules was partially hydrolyzed to form holes, dried at 120°C after centrifugation, and became micro Hole cornmeal.

[0067] 3) Add microporous corn...

Embodiment 3

[0073] 1) High-density culture of chitinase-producing Sb, the starting density of Sb is 1.95×10 6 Individuals / mL, inoculated into liquid culture medium (based on 1 L, glucose 14g, soybean powder 25g, shrimp powder 2g, the balance was water, adjusted to pH=7.0, sterilized at 121°C for 20min), the inoculum size was 3%, 37 Cultivate with shaking at ℃ for 24 hours, and the density of the bacterial solution is 6.24×10 9 cfu / mL to obtain a high-density Sb bacterial solution.

[0074] 2) Add corn flour to water and stir to make corn milk. The concentration of corn milk is 50% (that is, the mass ratio of corn flour to water is 50:50). Add α-amylase (EC.3.2.1.1) and glucoamylase (EC. 3.2.1.3) composed of raw amylase (enzyme dosages are 5U / L and 500U / L respectively), cultured with shaking at 37°C for 24hrs, the starch in the corn flour granules was partially hydrolyzed to form holes, dried at 120°C after centrifugation, and became micro Hole cornmeal.

[0075] 3) Add microporous corn...

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Abstract

The invention discloses a method for biologically preventing, controlling and reducing aphids on wolfberry and improving production performances. The method comprises culturing bacillus subtilis CICC10339 called as Sb for short, preparing a Sb bacterial liquid, carrying out enzymolysis on corn flour through raw amylase to obtain microporous corn flour, embedding the Sb in micropores of the microporous corn flour to obtain a microporous corn flour Sb bacterial liquid, preparing a Sb bacterial agent from the microporous corn flour Sb bacterial liquid, preparing Sb biological fertilizer throughthe Sb bacterial agent as a fermenting agent, spraying the Sb bacterial agent to the surface of a wolfberry plant to effectively reduce the aphids, and applying the Sb biological fertilizer to roots of the wolfberry plant to effectively reduce the aphids. The Sb for producing chitinase is used for eco-friendly biological prevention and control. Through various comprehensive supplementary means andcombination and synergism of the Sb bacterial agent and the Sb biological fertilizer, the aphids on wolfberry are effectively killed and the production performance of wolfberry is improved.

Description

technical field [0001] The invention relates to the field of biological control, in particular to a method for reducing wolfberry aphids and improving production performance through biological control. Background technique [0002] Lycium barbarum has pests and diseases in various stages of growth, and aphids are one of the main pests. When the air humidity is higher than 75%, it is not conducive to the reproduction of Lycium barbarum aphids. Therefore, Lycium barbarum aphids are more harmful in dry seasons. Excessive application of nitrogen fertilizers in production will also lead to the occurrence of aphid pests. Lycium barbarum aphids live through the winter with their eggs on the branches or at the roots of wolfberry plants on the ground. They start to damage wolfberry after they germinate in April, and flourish in May. Flowering and fruiting and growth and development cause the output of Lycium barbarum fruit to reduce by 30%-70%, the quality of the product is greatly ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N11/04A01N63/00A01P7/04A01G13/00C05G3/00C05G3/02C12R1/125C05G3/60
CPCA01G13/00A01N63/00C05D1/00C05G3/00C05G3/60C12N1/20C12N11/04C05F1/005C05F5/002C05F11/00
Inventor 阮晖胡超凡金迅邱亦亦丁丽娜
Owner ZHEJIANG UNIV
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