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Nitrocellulose microporous membrane with paper liner and preparation method thereof

A technology of nitrocellulose and microporous membrane, applied in chemical instruments and methods, membrane, membrane technology and other directions, can solve the problems of high crystallinity of natural cellulose, insoluble cellulose, non-plasticity and other problems, achieve smooth surface, preparation The effect of simple and easy process

Active Publication Date: 2018-09-07
RUNBIO BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the high crystallinity of natural cellulose and the existence of hydrogen bonds between and within the molecules, the cellulose is difficult to dissolve and melt. Lamination of nitrocellulose microporous membranes using only heat and pressure is not possible as a durable bond cannot be achieved
Furthermore, bonding the film to the carrier by thermal lamination is also cost-effectively an expensive multi-step process

Method used

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  • Nitrocellulose microporous membrane with paper liner and preparation method thereof
  • Nitrocellulose microporous membrane with paper liner and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] (1) Dissolve 9.2 parts by weight of nitrocellulose in 49.8 parts by weight of ethyl formate; then add 21.6 parts by weight of ethanol, 11.4 parts by weight of isobutanol, 7 parts by weight of deionized water and 1 part by weight of glycerol at 20-23 Stir evenly at ℃, degas and filter to obtain the nitrocellulose casting solution for later use.

[0040] (2) Inject about 20L of absolute ethanol as the soaking solution into the soaking tank in front of the casting device, and fully soak the front end of the roll of oil-absorbing filter paper through the guide roller into the soaking tank where the filter paper soaking solution is placed in advance. The specifications of the filter paper are as follows: quantitative: 280-320 g / m2, thickness: 0.45-0.55mm, pore size: 10-30μm.

[0041] (3) The filter paper soaked with saturated infiltration solution is pulled by the guide roller and fixed on the surface of the stainless steel conveyor belt, and is transported to the casting de...

Embodiment 2

[0045] (1) Dissolve 10.3 parts by weight of nitrocellulose in 50.8 parts by weight of acetone; then add 20.5 parts by weight of ethanol, 10.4 parts by weight of n-butanol, 7.5 parts by weight of deionized water and 0.5 parts by weight of Tween 20 at 15-30 Stir evenly at ℃, degas and filter to obtain the nitrocellulose casting solution for later use.

[0046] (2) Inject 20L of absolute ethanol plus 2L of acetone as the soaking solution into the soaking tank before the casting device, and immerse the front end of the roll of oil-absorbing filter paper through the guide roller into the soaking tank where the filter paper soaking solution is placed in advance to fully soak. The specifications of the filter paper are as follows: quantitative: 280-320 g / m2, thickness: 0.45-0.55mm, pore size: 10-30μm.

[0047] (3) The filter paper soaked with saturated infiltration solution is pulled by the guide roller and fixed on the surface of the stainless steel conveyor belt, and is transported...

Embodiment 3

[0051] Dissolve 9.8 parts by weight of nitrocellulose in 52.8 parts by weight of acetone; then add 16.5 parts by weight of ethanol, 4.5 parts by weight of isopropanol, 12.0 parts by weight of isobutanol, 4.0 parts by weight of deionized water, and 0.4 parts by weight of Qular Stir evenly with X-100 at 15-30°C, defoam and filter to obtain the nitrocellulose casting solution for later use.

[0052] Inject 20L of absolute ethanol plus 4L of ethyl formate as the soaking solution into the soaking tank before the tape casting device. The preparation process steps are repeated in Example 2 except that the coating thickness is 500 μm, and the nitrocellulose with the paper liner is obtained. Plain microporous membrane, visual test results are shown in Table 1.

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Abstract

The invention provides a method for producing a nitrocellulose microporous membrane with a paper liner, the surface of filter paper as a water absorbent pad is directly coated with a nitrocellulose casting solution, the dry filter paper as the water absorbent pad is placed in a filter paper immersion liquid for full immersion, and the nitrocellulose microporous membrane with the paper liner can beonce produced by phase conversion. The composite microporous membrane has a smooth surface, closely adheres to the surface of the filter paper, and can be used for a dot immunofiltration assay.

Description

technical field [0001] The invention relates to a nitrocellulose microporous membrane with a paper liner and a preparation method thereof, in particular to a nitrocellulose microporous membrane coated with a nitrocellulose microporous membrane layer on the surface of a water-absorbing filter paper containing porous spaces Membranes and methods for their preparation. Background technique [0002] The dot immunofiltration test was originally developed on the basis of the dot ELISA, and the conjugate used was enzyme-labeled, called the dot enzyme immunofiltration test. In the early 1990s, the dot immunogold filtration assay (DIGFA) with colloidal gold as a marker was developed, also known as the drop gold immunoassay (referred to as drop gold method). The drop gold method does not require the reaction of the enzyme to the substrate, which is simpler and faster, and is widely used in clinical testing. The specific test method takes the double-antibody sandwich method as an exa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01D67/00G01N33/53
CPCB01D67/0002B01D69/12B01D2325/36G01N33/53
Inventor 杨明京
Owner RUNBIO BIOTECH CO LTD