Method for extracting and purifying anthocyanin from chokeberry residues

A technology of chemical anthocyanins and aronia, applied in chemical instruments and methods, organic chemistry, azo dyes, etc., can solve the problems of natural anthocyanins loss, occupying a lot of space, long production cycle, etc., and achieve shelf life Long-term, high cost, and the effect of increasing added value

Inactive Publication Date: 2018-09-14
NANOLATTIX BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, a large amount of loss of natural anthocyanins often occurs during processing such as extraction and purification, and equipment investment is large.
The enzyme method has a long production cycle and takes up a lot of space

Method used

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  • Method for extracting and purifying anthocyanin from chokeberry residues
  • Method for extracting and purifying anthocyanin from chokeberry residues

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Embodiment 1: the specific operation steps of preparing high-quality aronia juice and extracting and purifying anthocyanins and polyphenols from aronia pomace are as follows:

[0030] (1) Choose berries that are round and full in shape and free from pests. Soak the selected aronia berries in artificial acidic oxidation potential water (pH 6.0 -7.0) for 20-30 minutes, then rinse them with clean water; beat them with a beater , to get aronia pulp;

[0031] After breaking the pulp, add 0.05-0.15% pectinase, 0.05-0.15% cellulase and 3-7% hemicellulase, bathe in 50°C water for 1.5h, stir once every 0.5h; No pectin was detected in the solution. The detection of pectin in aronia pulp solution was carried out by absolute ethanol or 95% ethanol. The specific operation is to take 2.5 milliliters of aronia pulp solution, add 7.5 milliliters of 95% ethanol, and shake. Because pectin is insoluble in ethanol, if there is pectin, there will be turbidity or precipitation; if there i...

Embodiment 2

[0046] Embodiment 2: the specific operation steps of extracting and purifying anthocyanins from Aronia juice residue are as follows:

[0047] Beating: aronia pulper for beating to obtain aronia juice;

[0048] Separation of aronia pulp: the aronia pulp is separated by a 300-mesh pulp separator with a filter cloth (centrifugal separation of aronia under the condition of 5100r / min);

[0049] Enzymolysis: add 10-20 times of volume of acidic oxidation potential water in the aronia pomace solution, adjust the pH of the aronia pomace solution to 4.5, and the temperature is 55°C; add the blueberry pomace quality (m / m) in the aronia pomace solution 0.3% pectinase, mixed evenly, and enzymatically hydrolyzed for 6 hours, until no pectin was detected in the aronia pomace solution. The detection of pectin in aronia pomace solution was performed by absolute ethanol or 95% ethanol. The specific operation is to take 2.5 ml of aronia pomace solution, add 7.5 ml of 95% ethanol, and shake. B...

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Abstract

The invention belongs to the technical field of natural pigment extraction and purification and provides a method for extracting and purifying anthocyanin from chokeberry residues. The method for extracting and purifying the anthocyanin from the chokeberry residues is aimed at solving problems existing in the prior art of chokeberry juice preparation and anthocyanin extraction. The method comprises, after chokeberry is washed, removing acrid taste of the chokeberry through artificially electrolyzed acid reductive water, tannase and chitosan, processing the chokeberry residues as well as extracting the anthocyanin through the electrolyzed acid reductive water, stirring in acidic electrolyzed oxidizing water, soaking and separating out the chokeberry residues for precipitation, filtering andmembrane-filtering anthocyanin aqueous solution for multiple times for repeated extraction until extracting solution is colorless or light-colored, combining extracting solution and performing concentration and drying processes to obtain extractives; absorbing, eluting and concentrating filtering liquor to obtain paste anthocyanin; preparing solid anthocyanin through conventional methods; preparing feedstuff and fertilizers with waste residues. The method for extracting and purifying the anthocyanin from the chokeberry residues is simple and easy to industrialize, reduces consumption of chemical agent and water, improves the yield of products, achieves comprehensive utilization of the chokeberry and increases the value added as well as economic benefits, social benefits and environmentalprotection benefits.

Description

technical field [0001] The invention belongs to the technical field of extraction and purification of natural pigments, in particular to a method for extracting and purifying anthocyanins from aronia berry pomace. Background technique [0002] aronia ( Aronia melanocarpa ), the scientific name is Sorbus nigra, which is native to wet forests and swamps in eastern North America. Since 1989, my country has introduced 8 varieties from abroad. Up to now, my country has rich planting resources. Aronia fruit is rich in polyphenols, sugars, organic acids, minerals, vitamins and various trace elements, among which the content of polyphenols is the highest among fruits. In recent years, domestic research on its function has increased. Its fruit juice has strong antioxidant properties, as well as anti-cancer, anti-inflammatory, prevent urinary tract infection, treat diabetes, protect the liver, and treat obesity. It has a remarkable effect on cerebrovascular diseases. Regular consump...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D311/62C09B61/00C09B67/54
CPCC07D311/62C09B61/00C09B67/0096
Inventor 李少平渠志灿梁锐
Owner NANOLATTIX BIOTECH CO LTD
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