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Method of analyzing succession of microbial communities on soil bio-film

A technology for microbial communities and soil organisms, applied in the field of analyzing the succession of soil biofilm microbial communities, which can solve the problems of difficulty in studying microbial succession and few study microorganisms.

Inactive Publication Date: 2018-09-14
HUAZHONG AGRI UNIV
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  • Abstract
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  • Application Information

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Problems solved by technology

[0006] Succession is an important part of studying ecology. It is a biological change process of a certain habitat in a specific environment. Succession is widely studied in plant and animal ecosystems, but microorganisms are widely distributed in nature, but Microbial succession has rarely been studied
This is due to the difficulty of studying microbial succession due to the limitations of traditional methods that rely on laboratory culture of microorganisms

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  • Method of analyzing succession of microbial communities on soil bio-film
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  • Method of analyzing succession of microbial communities on soil bio-film

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Embodiment Construction

[0030] The present invention will be described in detail below through specific examples.

[0031] 1. Materials and methods

[0032] 1.1 Materials

[0033] 1.1.1 Test soil

[0034] The soils for the test were collected from two adjacent paddy fields in Dajin Town, Wuxue City, Hubei Province. The farming methods were the same, but the rice yields were significantly different, so they were named high-yield field soil and low-yield field soil respectively. The sample was collected in November 2015. The sample was rhizosphere soil. The S-shaped line was used for random sampling, and the mixed soil sample was collected. The gravel and rice residual roots were removed and put into a sealed bag, sealed and stored at low temperature, and brought back to the laboratory.

[0035] 1.1.2 Reagents used in soil DNA extraction

[0036] DNA extraction solution: 100mM Tris-HCl (pH8.0), 100mM Na-EDTA (pH8.0), 100mM sodium phosphate buffer solution (pH8.0), 1.5M NaCl, 1% / 3% CTAB (hexadecyltri...

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Abstract

The invention discloses a method of analyzing succession of microbial communities on soil bio-film, comprising: preparing an initial inoculating liquid, establishing a flow cell bio-film, analyzing dynamic composition and diversity of communities on the soil bio-film, separating and identifying culturable bacteria in the soil bio-film, and the like. A model to study soil bio-film diversity and early succession is established herein; the flow culture bio-film composed of soil microbes is assembled; the PCR (polymerase chain reaction) technique and high-throughput sequencing analysis are utilized to classify the microbes, strains that dominate in different periods of time are separated, and research on the succession of microbial communities on the soil bio-film is achieved accordingly.

Description

technical field [0001] The invention belongs to the field of microorganisms, and relates to a method for analyzing soil biofilm microbial community succession. Background technique [0002] In soil, the vast majority of microorganisms are distributed on solid surfaces such as soil minerals and rhizosphere, forming micro-colonies or biofilms. Bacterial biofilm is a cell aggregate formed by bacteria adsorbed on the surface of inert or active materials in order to adapt to the living environment during the growth process. It is composed of bacteria and their own extracellular matrix. important way of life. [0003] Biofilm cells are completely different from free cells in many aspects. The gene expression of cells forming biofilms is significantly different from that of free cells. The expression patterns of cells in biofilms are more complex than those of free cells. Biofilms and free cells Compared with cells, the extracellular substance EPS secreted by bacteria can protect...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6869C12Q1/04
CPCC12Q1/6869C12Q2531/113C12Q2535/122
Inventor 蔡鹏高春辉曹慧吴一超黄巧云李准洁
Owner HUAZHONG AGRI UNIV
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