Method and device for rapidly detecting quantity of reticulocytes in peripheral blood

Abstract

The invention discloses a method for rapidly detecting the quantity of reticulocytes in peripheral blood. The method comprises the following steps: 1, diluting a blood sample: accurately diluting a certain amount of the peripheral blood with normal saline for 50 to 100 times to obtain a diluted blood sample; step 2, carrying out fluorescent staining on the diluted blood sample obtained by step 1 to obtain a stained blood sample; 3, generating cell-in-oil liquid drops containing a single cell; 4, capturing the cell-in-oil liquid drops: putting all the cell-in-oil liquid drops into detection micro-pores, wherein each detection micro-pore contains one cell-in-oil liquid drop at most; 5, detecting the quantity of the reticulocytes; 6, calculating the content of the reticulocytes in the peripheral blood sample with a unit volume according to the quantity of the reticulocytes, which is detected by a detection plate. Meanwhile, the invention discloses a device for rapidly detecting the quantity of the reticulocytes in the peripheral blood.

Description

technical field [0001] The invention relates to the field of counting reticulocytes in peripheral blood, in particular to a method and device for rapidly detecting the number of reticulocytes in peripheral blood. Background technique [0002] Human peripheral blood contains a very small amount of immature reticulocytes, whose morphological feature is the residual part of basophilic substance (RNA) in the cytoplasm of the cells. At present, the methods for detecting reticulocytes mainly include the following: (1) Manual microscopic examination method: the erythrocytes in the blood sample are pushed, stained, and dried to obtain cell slices with brilliant tar blue staining solution, and then examined under a microscope Make observations. The observer selects the part on the preparation where the cell distribution is relatively uniform and the thickness is moderate, observes and counts under the oil lens of the microscope (1000 times magnification). The artificial microscope ...

AI Technical Summary

Problems solved by technology

This method has a high degree of automation, and compared with manual microscopic examination, the efficiency is significantly improved, but there are still the following deficiencies: First, the RNA in reticulocytes is distributed in a network or cluster shape, and after the RNA is combined with a fluorescent dye, the fluorescent signal Scattered distribution, when the cells pass the detector, it is difficult to distinguish individual cells based on the fluorescent signal

Second, the design of the solution pipeline of the flow cytometer is complicated, the cost is high, and it must be reused, which easily leads to cross-contamination between samples

3. The flow cytometer uses the principle of sheath flow, and the cells must pass through the fluorescence detection mechanism one by one, resulting in a slowdown of the test speed.

Images