Trace and rapid method for determining content of algae oil

Abstract

The invention discloses a trace and rapid method for determining the content of algae oil. The method comprises the following steps: (1) taking algae liquid and calculating the quantity concentrationof algae cells in the algae liquid; (2) diluting to obtain algae solutions with different algae concentrations; (3) preparing a mother solution; (4) taking 2 test tubes according to each algae concentration; adding 5 to 10mL of the algae liquid and 5 to 10ml of HCl with the concentration of 1mol / L respectively; after sufficiently mixing, adding 0.1mul of the mother solution into each branch tube according to each algae concentration and adding an organic solvent in step (3) into the other branch tube; taking the branch tubes as contrast tubes and sufficiently mixing; (5) observing under a fluorescence microscope by utilizing exciting light with the exciting wavelength of 400 to 600nm, and photographing and recording a single-cell oil accumulation and change condition; (6) calculating an oil volume; (7) calculating the oil content of the algae liquid with a unit volume. By adopting the method disclosed by the invention, the detection is rapid and simple. According to the method, microalgae culture and a detection method are fused together and a process is simple and convenient; the microalgae oil content is determined through vivo detection; compared with the time of a conventionalmethod, the needed time is remarkably shortened.

Description

technical field [0001] The invention relates to the field of microalgae bioenergy, in particular to a method for quickly measuring the content of algae oil in a small amount. Background technique [0002] The progress of human civilization largely depends on the development of energy. At present, the energy used by human beings is dominated by fossil fuels, and the rapid growth of civilization economy has led to a rapid increase in human demand for energy. The energy crisis in the world is deepening today, and the shortage of fossil fuels and the environmental problems it brings are becoming increasingly prominent. Therefore, looking for environmentally friendly renewable energy has become a concern of all countries in the world. As a renewable and environmentally friendly new energy source, biodiesel has attracted much attention, and its source is microalgae, which has attracted the most attention because microalgae has the advantages of high oil production, fast growth and...

AI Technical Summary

Problems solved by technology

This method greatly reduces sample consumption and measurement time, and is simple and easy to implement, but expensive fluorescent dyes and detection equipment limit the application of this method in production, and in addition, it requires highly toxic dimethyl sulfoxide (DMSO) or acetone As a dyeing solution solvent, it is not conducive to practical operation