Detection test strip capable of remarkably improving lateral flow immunochromatography

A technology of immunochromatographic detection and immunoassay, which is applied in the direction of measuring devices, analytical materials, instruments, etc., can solve the problems of high detection sensitivity, low detection limit, and short mixing time in vitro

Active Publication Date: 2018-09-14
上海艾瑞德生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] In summary, there is still a lack of an immunochromatographic test strip with high detection sensitivity, short in vitro mixing time, and low detection limit in this field.

Method used

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  • Detection test strip capable of remarkably improving lateral flow immunochromatography
  • Detection test strip capable of remarkably improving lateral flow immunochromatography
  • Detection test strip capable of remarkably improving lateral flow immunochromatography

Examples

Experimental program
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Effect test

Embodiment 1

[0087] Take the determination of troponin cTnI standard as an example.

[0088] Use anti-cTnI mouse monoclonal antibody to label fluorescent microspheres, use anti-cTnI goat polyclonal antibody as capture antibody to draw on the membrane as a detection line, and use goat anti-mouse polyclonal antibody as quality control line to draw membrane antibody. The cTnI standard substance and the fluorescent microspheres of the labeled antibody were mixed in vitro for 30 seconds, then dropped onto the sample pad, and then measured after lying flat for 900 seconds. The probe of the detector moves from the direction of the sample pad to the direction of the absorbent pad, and a total of 180 points including the T line and the C line are read.

[0089] Schematic such as Figure four , calculate the values ​​of TY and CY by computer, integrate the peak area of ​​T line and C line to get the values ​​of TA and CA, and use TA / TA+CA to calculate the value of TAP to calibrate the difference ca...

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Abstract

The invention provides a method for remarkably improving the strength of a detection signal of a lateral flow immunochromatography detection test strip. The method for improving the strength of the detection signal comprises the following steps: (a) after mixing a sample and an antibody marked on a fluorescent microsphere in vitro, adding a mixture onto a sample pad of chromatography test paper; (b) after combining a matter to be analyzed in the sample with the antibody marked on the fluorescent microsphere, forming a compound to be detected; gradually dispersing the compound to be detected toa nitrocellulose membrane along a chromatography flow; (c) dividing a detection line and a quality control line on the nitrocellulose membrane; (d) carrying out perforation modification on the nitrocellulose membrane between the sample pad and the detection line to obtain a modification region formed by 1 to 10 rows of modification holes. According to the method provided by the invention, the aims of increasing the probability of capturing the compound to be detected, reducing the time of uniformly blending in vitro, improving the testing sensitivity, reducing the measurement limit value and / or improving the detection signal can be realized.

Description

technical field [0001] The invention relates to an immunochromatography test strip, in particular to a lateral flow immunochromatography test strip which can significantly improve the lateral flow immunochromatography. Background technique [0002] Lateral flow chromatography has the advantages of low cost, simple operation, quickness, and portability, and is an important part of the field of medical diagnosis. [0003] At present, the common lateral flow immunochromatography method on the market is mainly the double-antibody sandwich method, also known as the sandwich detection method, which consists of a polyvinyl chloride bottom plate, a nitrocellulose membrane, a sample pad, and an absorbent pad. Generally, in lateral flow immunofluorescence chromatography, human or animal body fluid samples are mixed with fluorescent particle-labeled antibodies in vitro to form antigen-antibody complexes, which are added to the sample pad, and the complexes diffuse with the chromatograp...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/533
CPCG01N33/533
Inventor 李秋萍王佩瑞叶一肖琨倪晓涛陆亮朱轩仪杨茜茹操凤文李欢
Owner 上海艾瑞德生物科技有限公司
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