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Correction and Compensation Method of Background Nonuniformity in Differential Phase Contrast Microscopy

A microscopic imaging and non-uniformity technology, applied in the field of image correction, can solve the problem of uneven brightness and darkness of images, and achieve the effect of correcting image non-uniformity, the correction process is simple and fast, and the imaging quality is improved

Active Publication Date: 2022-01-07
NANJING UNIV OF SCI & TECH
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Problems solved by technology

[0005] The purpose of the present invention is to provide a method for correcting and compensating background non-uniformity in differential phase contrast microscopic imaging, so as to solve the problem of uneven brightness and darkness of the image that exists when the differential phase contrast imaging system collects images, and to ensure that the differential phase contrast Based on the imaging speed of the imaging microscope system, the imaging quality and phase solution accuracy are improved

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  • Correction and Compensation Method of Background Nonuniformity in Differential Phase Contrast Microscopy
  • Correction and Compensation Method of Background Nonuniformity in Differential Phase Contrast Microscopy

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[0026] The actual hardware platform on which the correction and compensation method for background non-uniformity in the differential phase contrast microscopic imaging of the present invention relies is a microscope system based on a programmable LED array, and the whole system includes an image acquisition camera, a microscopic objective lens, a sample, and an object stage , Programmable LED light source. As the illumination source of the microscope, the LED array is placed directly under the sample stage, the distance H from the upper surface of the stage is generally between 20-100mm, and the center of the LED array is on the optical axis of the microscope objective lens . The LED array includes several LED elements, which are arranged regularly to form a two-dimensional array. Among them, the single LED elements are all red, green and blue three-color LEDs, and their typical wavelengths are red light 635nm, green light 525nm and blue light 475nm. The center distance d b...

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Abstract

The invention discloses a correction and compensation method for background non-uniformity in differential phase-contrast microscopic imaging. The background pre-calibration or self-adaptive background fitting method is adopted. In the background image, the non-uniformity of the image is eliminated by subtraction or division in subsequent calculations. In differential phase contrast imaging, the inhomogeneity of the image is distributed linearly in the direction of illumination. Based on this, the adaptive background fitting method adopts three kinds of fitted background images, which are eliminated by subtraction or division in subsequent calculations. Image non-uniformity. The present invention solves the problem of uneven brightness and darkness of images that exist when a differential phase-contrast imaging system collects images, and improves imaging quality and phase resolution accuracy on the basis of ensuring the imaging speed of the differential phase-contrast imaging microscope system.

Description

technical field [0001] The invention belongs to the field of image correction of optical microscopic imaging technology, in particular to a correction and compensation method for background non-uniformity in differential phase contrast microscopic imaging. Background technique [0002] As the basic unit of organism composition and life activities, the accurate, clear and quantitative reproduction of its structure has always played a vital role in the research of biomedicine and life sciences. However, most biological cells are colorless and transparent, and have weak absorption in the visible light band, and the light intensity will not cause obvious changes after the light passes through the cells, which makes cell observation impossible. [0003] In the field of microscopic technology, there are two ways to solve this problem. The first method uses "marking", relying on dyes to change the weak absorption properties of cells to achieve the visibility of cells under visible ...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G06T7/73G06T5/00G06T5/50
CPCG06T5/006G06T5/50G06T7/74
Inventor 陈钱范瑶左超孙佳嵩冯世杰张玉珍顾国华李加基张佳琳丁君义
Owner NANJING UNIV OF SCI & TECH
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