Correction and Compensation Method of Background Nonuniformity in Differential Phase Contrast Microscopy

Abstract

The invention discloses a correction and compensation method for background non-uniformity in differential phase-contrast microscopic imaging. The background pre-calibration or self-adaptive background fitting method is adopted. In the background image, the non-uniformity of the image is eliminated by subtraction or division in subsequent calculations. In differential phase contrast imaging, the inhomogeneity of the image is distributed linearly in the direction of illumination. Based on this, the adaptive background fitting method adopts three kinds of fitted background images, which are eliminated by subtraction or division in subsequent calculations. Image non-uniformity. The present invention solves the problem of uneven brightness and darkness of images that exist when a differential phase-contrast imaging system collects images, and improves imaging quality and phase resolution accuracy on the basis of ensuring the imaging speed of the differential phase-contrast imaging microscope system.

Description

technical field [0001] The invention belongs to the field of image correction of optical microscopic imaging technology, in particular to a correction and compensation method for background non-uniformity in differential phase contrast microscopic imaging. Background technique [0002] As the basic unit of organism composition and life activities, the accurate, clear and quantitative reproduction of its structure has always played a vital role in the research of biomedicine and life sciences. However, most biological cells are colorless and transparent, and have weak absorption in the visible light band, and the light intensity will not cause obvious changes after the light passes through the cells, which makes cell observation impossible. [0003] In the field of microscopic technology, there are two ways to solve this problem. The first method uses "marking", relying on dyes to change the weak absorption properties of cells to achieve the visibility of cells under visible ...

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Problems solved by technology

[0005] The purpose of the present invention is to provide a method for correcting and compensating background non-uniformity in differential phase contrast microscopic imaging, so as to solve the problem of uneven brightness and darkness of the image that exists when the differential phase contrast imaging system collects images, and to ensure that the differential phase contrast Based on the imaging speed of the imaging microscope system, the imaging quality and phase solution accuracy are improved

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