Lotus root detoxication tissue culture method

Abstract

The invention discloses a lotus root detoxication tissue culture method, which comprises the following steps of (1) raw material selection: selecting non-rotten healthy and strong lotus root tubers; (2) germination acceleration; performing germination acceleration on the selected lotus root tubers; (3) surface treatment; (4) cutting; (5) primary generation culture; (6) proliferation subculture; (7) rooting; (8) domestication seedling exercising: coating root parts of lotus root sterile young seedlings with the developed root systems by half wet moss; putting the seedlings into a seedling pot;transferring the seedling pot into a small greenhouse for domestication. The method provided by the invention has the beneficial effects that culture mediums, the culture temperature and the illumination time influencing the fast breeding of the lotus root tissue culture seedlings, the seedling exercising method and the like are studied; the problems about detoxication, quality degradation and breeding coefficient improvement are successfully solved; the product yield and the product quality are improved; the lotus root export foreign exchange earning and income increase of farmers can be realized.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a lotus root detoxification tissue culture method. Background technique [0002] Lotus root is a perennial aquatic herbaceous plant of Nymphaeaceae. It is one of the characteristic aquatic vegetables widely cultivated in China and one of the important foreign exchange earning vegetables for export. [0003] In recent years, the planting area of ​​lotus root in my country has continued to expand, and the products are increasingly sold in foreign markets. However, since lotus root has been asexually propagated through rhizomes for a long time, its reproduction coefficient is low, it requires a large amount of species, and the cost is high, and it is prone to species degradation. Phenomena, the occurrence of diseases and insect pests also has an increasingly serious trend, which seriously affects the output and quality of products, and also limits the further...

AI Technical Summary

Problems solved by technology

At present, there are few patent reports on the tissue culture method of lotus root. In Yangzhou University Xie Ke’s master’s thesis "Research on the Efficient Propagation Technology of Lotus Root Tissue Culture Seedlings", the tissue culture method of lotus root was preliminarily discussed, but most of the researches were only in the laboratory stage and could not be obtained. Solving problems in the actual production process has certain limitations