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Preparation method of microbial enhanced flocculation algaecide

The technology of algaecide and microorganism is applied in the field of preparation of microbial enhanced flocculation algaecide.

Active Publication Date: 2021-12-03
HUATIAN ENG & TECH CORP MCC +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The traditional flocculation materials are mainly based on clay, compounded with polyaluminum chloride, polyferric chloride and chitosan modifier to prepare flocculation and algae removal materials, but only using chemical flocculants to remove algae is not conducive to Improve the algae inhibition ability of the water body itself, prolonging the biological ecological restoration cycle

Method used

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  • Preparation method of microbial enhanced flocculation algaecide
  • Preparation method of microbial enhanced flocculation algaecide
  • Preparation method of microbial enhanced flocculation algaecide

Examples

Experimental program
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Effect test

Embodiment 1

[0024] The present invention provides a kind of preparation method of microbial enhanced flocculation algicide, comprising the following steps:

[0025] The carrier material is modified by 0.1-10mg / mL chitosan aqueous solution and then dried, and the antibacterial microorganisms are dissolved in sodium alginate aqueous solution to obtain a density of antibacterial microorganisms greater than 10 6 cells / mL of sodium alginate bacteria solution, spray or dropwise add sodium alginate bacteria solution to the modified carrier material and mix well to obtain the flocculation algaecide precursor; The algaecide precursor is dried, fully ground through a 60-400 mesh sieve, and made into a flocculation algaecide strengthened by microbial cells.

[0026] In practical application, sodium alginate bacteria solution can be dissolved by dissolving 0.5-2% sodium alginate in bacteria density>10 6 cells (cells) / mL of microbial inoculum.

[0027] The bacteriostatic microorganisms mentioned in ...

Embodiment 2

[0031] combine figure 1 , as a specific scheme of the above-mentioned embodiment, a preparation method of a microbial enhanced flocculant is provided in this embodiment, the steps are:

[0032] (1), obtain Bacillus Laterosporus, after rejuvenating and activating Bacillus Laterosporus, ferment with LB liquid medium for 12-24h, wash and collect the thalline cells, and concentrate to obtain a cell density of 10 11 cells / mL;

[0033] (2), dissolve the sodium alginate aqueous solution of 1% with Bacillus lateralosporus thalline cell, the thalline cell density is 10 11 cells / mL, to obtain sodium alginate antibacterial solution;

[0034] (3), prepare chitosan aqueous solution: add 3g chitosan to 500mL mass fraction and be in the acetic acid solution of 5%, add distilled water to 2L after constantly stirring to make it dissolve, obtain the chitosan aqueous solution of 1.5mg / mL;

[0035] (4), preparation of modified carrier material: the diatomite is pulverized, ground and sieved to...

Embodiment 3

[0039] combine figure 2 , as another specific solution of embodiment 1, the preparation method of microbial enhanced flocculant in this embodiment includes:

[0040] (1) Obtain Bacillus subtilis, rejuvenate and activate the Bacillus subtilis, ferment with LB liquid medium for 12-24h, wash and collect the bacterial cells, concentrate to obtain a cell density of 1011 cells / mL;

[0041] (2), be dissolved in the sodium alginate aqueous solution of 0.8% with subtilis thalline cell, the thalline cell density is to 10 11 cells / mL, to obtain sodium alginate antibacterial solution;

[0042] (3), prepare chitosan aqueous solution: add 5g chitosan to 500mL mass fraction and be in the hydrochloric acid solution of 1%, add distilled water to 5L after constantly stirring to make it dissolve, obtain the chitosan aqueous solution of 1.0mg / mL;

[0043] (4), preparation of modified carrier material: sepiolite powder pulverized, ground and sieved to obtain a particle size of 150 mesh sepiolit...

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Abstract

The invention discloses a preparation method of a microbial enhanced flocculation algaecide. It is mainly designed to shorten the cycle of ecological restoration of water bodies, including the following steps: (1) Prepare modified carrier material and sodium alginate anti-algae liquid; (2) Add sodium alginate anti-algae liquid to the modified carrier material and mix After stirring, a mixture is formed, and the mixture is incubated to obtain a flocculation algaecide precursor; (3) the flocculation algaecide precursor is dried, ground and sieved to obtain a flocculation algaecide. The preparation method of the microbial flocculation algaecide of the present invention utilizes the algae-inhibiting function of microbial cells to strengthen the flocculation effect, puts the algae-inhibiting microorganisms into the water body to form competition with algae, limits the excessive reproduction of algae, improves the transparency of the water body, and enhances the inhibition of the water body. Algae ability, effectively respond to water quality problems caused by sudden algal blooms, and shorten the restoration cycle of water bodies.

Description

technical field [0001] The invention belongs to the technical field of environmental protection, and in particular relates to a preparation method of a microbial enhanced flocculation algaecide. Background technique [0002] The algae bloom phenomenon caused by water eutrophication increases the cost of water treatment and maintenance, and makes the water ecological environment unbalanced, which has become one of the important challenges facing the field of water resources and environment. [0003] Adding flocculant to remove algae cells in the water body of algae bloom outbreak is one of the most direct and effective methods for emergency treatment of cyanobacteria outbreak. The traditional flocculation materials are mainly based on clay, compounded with polyaluminum chloride, polyferric chloride and chitosan modifier to prepare flocculation and algae removal materials, but only using chemical flocculants to remove algae is not conducive to Improve the algae inhibition abi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C02F3/34
CPCC02F3/34C02F3/347
Inventor 赵丹孟溪冯植飞骆守鹏程寒飞
Owner HUATIAN ENG & TECH CORP MCC