Rapid detection method of plant root system activity

Abstract

The invention relates to the field of plant nutrition and physiology, and discloses a rapid detection method for plant root system activity. The method comprises the following steps: S1: a plant rootsystem sample is immersed in a mixed solution of a TTC solution and a phosphatic buffer solution, and darkly insulated for 1-2 h at 37 DEG C, the reaction is terminated by adding sulfuric acid, and atthe moment, the plant root system sample turns red; S2: the red plant root system sample is transferred to an extract liquid prepared from ethyl acetate and methanol, after the sample is leached andextracted in a water bath at 55-60 DEG C for 15-25 min, the faded and whitened plant root system sample is taken out, and the reddened extract liquid is fixed to a volume by the extract liquid; S3: ablank test is taken as a control, the absorbance of TTF in the red extract liquid at the wavelength of 485 nm is detected by a spectrophotometer, and then the reduction amount of the TTC solution is calculated according to the standard curve. The method has a fast and efficient leaching effect on the TTF, and is good in reproducibility, easy to operate, and more suitable for batch detection of samples.

Description

technical field [0001] The invention relates to the field of plant nutrition and physiology, in particular to a rapid detection method for plant root activity. Background technique [0002] The plant root system is the organ that absorbs water and nutrients and fixes the plant. At the same time, the root system also has important synthetic and metabolic functions. Exuberant root activity has a direct and important impact on crop growth and yield formation, and is a key factor restricting the further development of crop yield potential. Therefore, root activity is a comprehensive physiological index that reflects the absorption function of roots and promotes the formation of photosynthetic substances. [0003] The determination of root activity mainly includes methylene blue method, α-naphthalene method, TTC (triphenyltetrazolium chloride) method and other methods. Because the results of TTC method are accurate and reproducible, it is widely used at present. [0004] The t...

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Problems solved by technology

[0007] (1) For the traditional grinding method, due to the need for grinding, rinsing, filtering, constant volume and other steps, the operation is cumbersome and time-consuming, which is not conducive to the detection of root vigor of large-scale samples;

[0008] (2) In the process of extracting TTF by traditional grinding method, it is precisely because of grinding, filtration and other links that often lead to the loss of TTF, resulting in a decrease in the extraction rate of TTF in the sample, thus affecting the reliability of the experimental results;

[0009] (3) Although the reported extraction method avoids the disadvantage of low extraction rate of the traditional grinding method, the extraction process of TTF generally takes 4-6 hours or longer, and a single methanol or dimethyl methylene The extraction efficiency of TTF by sulfone extract is not satisfactory

Therefore, this method is not conducive to the rapid detection of root vigor in large batches of samples.

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