Yellow-feather-broiler semen diluent and preparation and application methods thereof

A technology of yellow feather broiler and diluent, which is applied in the application, preservation of human or animal body, animal husbandry, etc., can solve the problem of cumbersome configuration of semen diluent, achieve a significant dilution effect, do not affect the insemination rate, and improve the production of enterprises. effect of benefit

Inactive Publication Date: 2018-09-25
常州市四季禽业有限公司
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  • Summary
  • Abstract
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Problems solved by technology

[0009] The purpose of the present invention is to provide a yellow-feathered broiler semen diluent and ...

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  • Yellow-feather-broiler semen diluent and preparation and application methods thereof

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Effect test

Embodiment 1

[0045] The packing and using method of the yellow feather broiler seminal fluid dilution that embodiment 1 makes:

[0046] Each diluent configuration is operated by a special person and is divided into 10ml glass bottles with scales. Each bottle is filled with 3.5ml diluent, and then each chicken house receives a small bottle of diluent according to the amount of insemination in the afternoon. Similarly, when collecting semen, the breeder uses the semen collection cup to collect the semen, and then pours the semen into a glass bottle with diluent (collect one and pour one semen to prevent chicken manure contamination during semen collection), and collect 3-4 chickens After the rooster semen, you can gently shake and mix the diluent and semen in the glass bottle. When collecting 3.5ml semen at a ratio of 1:1, you can mix the liquid in the glass bottle again. Use a single gun for insemination. The insemination volume is controlled at 0.025ml / body, and a bottle of diluted semen...

Embodiment 1

[0047] The use effect evaluation of the yellow feather broiler seminal fluid dilution that embodiment 1 makes:

[0048]The purpose of this experiment is to study the effects of insemination with diluted semen and original semen on the live embryo rate, dead embryo rate and light egg rate of eggs, so as to evaluate the use effect of the semen diluent. Divide the hens in the hen house into two groups, of which 6 in the south are the test group for dilute semen insemination, and 6 in the north are the control group for the original semen insemination. Unified use of back massage method of semen collection. Artificial insemination began after 2 p.m. every day. The test group’s 6 inseminations were divided into three days. The diluent and semen were diluted 1:1. The amount of insemination was 0.023ml / insemination, once every 6 days. The control group was inseminated with raw semen, and the dose, interval and frequency were the same as those of the test group. The test results s...

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Abstract

The invention relates to the technical field of semen processing, in particular to a yellow-feather-broiler semen diluent and preparation and application methods thereof. The preparation method includes the steps of firstly, weighing 3.15-3.85 parts of glucose, 2.93-3.58 parts of sodium glutamate, 0.81-0.99 part of potassium dihydrogen phosphate, 5.4-6.6 parts of sodium phosphate dibasic dodecahydrate and 0.22-0.28 part of ethylenediamine tetraacetic acid disodium; sequentially adding the materials above into a volumetric flask, adding distilled water for dissolving, evenly mixing, and allowing the solution to reach constant volume; thirdly, filtering the solution, boiling for sterilizing, cooling, and storing at low temperature. The yellow-feather-broiler semen diluent has the advantagesthat dilution rate can reach 1:2, and an evident dilution effect is achieved; after the semen diluent is used for insemination, the fertility rate of hens is increased as compared with that of original semen; the semen diluent is flexible and convenient to prepare and long in storage time and does not affect insemination rate.

Description

technical field [0001] The invention relates to the technical field of semen treatment, in particular to a diluent of chicken semen and a method for preparing and using the same. Background technique [0002] With the rapid development of the chicken industry in China, the adoption of artificial insemination technology is the only way for the chicken industry to standardize and standardize. Semen dilution is a mixed liquid that can reduce the metabolic rate of sperm and facilitate the storage and transportation of the semen of high-quality cocks. It is an important link in the development of artificial insemination technology for breeders. Its quality directly affects the fertilization rate and hatching of eggs. The level of the rate. As we all know, due to the large number of hens, the high frequency of insemination, and the low ejaculation volume of roosters, high sperm density, and short survival time of fresh semen outside the body, the semen needs to be diluted. But a...

Claims

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Application Information

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IPC IPC(8): A01N1/02
CPCA01N1/0221A01N1/0226
Inventor 张晓斌何小伟周新生熊平文张小聪薛木兰
Owner 常州市四季禽业有限公司
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