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Method for guiding sterilization and preservation of freshly squeezed caulis dendrobii juice by using metagenomic sequencing data

A technology of metagenomic and sequencing data, which is applied in the field of using metagenomic sequencing data to guide the sterilization and preservation of freshly squeezed Dendrobium dendrobium juice, and achieves the effects of shortening the screening period, expanding the scope of application, and reducing experimental consumption.

Pending Publication Date: 2018-09-28
HEFEI UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, since it is not sterilized, fresh-squeezed juice is mostly limited to being squeezed and used immediately. The sterilization and preservation of Dendrobium fresh juice is a bottleneck in its industrial production, and its sterilization and preservation process urgently needs a breakthrough

Method used

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  • Method for guiding sterilization and preservation of freshly squeezed caulis dendrobii juice by using metagenomic sequencing data
  • Method for guiding sterilization and preservation of freshly squeezed caulis dendrobii juice by using metagenomic sequencing data
  • Method for guiding sterilization and preservation of freshly squeezed caulis dendrobii juice by using metagenomic sequencing data

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] In the present embodiment, the method of using metagenomic sequencing data to screen Dendrobium officinale fresh juice antiseptic preservation process is as follows:

[0035] 1. Rinse and drain 80 g of Dendrobium candidum fresh strips and cut them into 1-2 cm long dendrobium segments; add 40 times the mass of pure water and beat for 2-3 minutes to make dendrobium juice. The dendrobium juice obtained in this step is not After sieve filtration or centrifugal separation treatment; followed by vacuum degassing (vacuum degree 0.08-0.1MPa, 2-3min), high-pressure homogenization (25MPa, 3min), PE / PA composite packaging bag packaging and sealing, each bag 50mL; put part of the dendrobium fresh juice into the ultra-high pressure processing equipment, hold the pressure at 300MPa for 15min, and the ultra-high pressure dendrobium juice is finished after the pressure is released. The ultra-high pressure dendrobium juice is stored at room temperature (20-25°C).

[0036] 2. Perform me...

Embodiment 2

[0042] In the present embodiment, the preparation method of Dendrobium officinale juice of Nisin-Natamycin synergistic ultra-high pressure sterilization is as follows:

[0043] 1. Wash and drain 50g fresh dendrobium strips, cut into dendrobium segments with a length of about 2cm, and put them into a wall-breaking beater; add 2L of pure water with Nisin (150mg / L)-Natamycin (50mg / L) , beating for 2-3 minutes to obtain dendrobium raw pulp juice, the juice obtained in this step is not filtered through a screen or centrifuged;

[0044] 2. Vacuum degas the freshly squeezed dendrobium juice obtained in step 1, and keep it for 2-3min at a vacuum degree of 0.08-0.1MPa; then homogenize under high pressure at 25MPa for 1-2 times, each time for 5 minutes, and homogenize the freshly squeezed dendrobium juice The juice is divided into PE / PA composite packaging bags, each bag is 50mL, and vacuum-sealed;

[0045] 3. Put the dendrobium fresh juice packaged in step 2 in ultra-high pressure pro...

Embodiment 3

[0047] In the present embodiment, the preparation method of the fresh juice of Dendrobium nobile in cooperation with ultra-high pressure sterilization by Nisin-Natamycin is as follows:

[0048] 1. Clean and drain 40g of fresh strips of Dendrobium nobile, cut into 1-2cm dendrobium segments, and put them into a broken wall beater; 2L of water, beating for 2-3 minutes to obtain the dendrobium juice; the dendrobium juice obtained in this step is not filtered through a sieve or centrifuged;

[0049] 2. Vacuum degas the freshly squeezed dendrobium juice obtained in step 1, and keep it for 2-3min at a vacuum degree of 0.08-0.1MPa; then homogenize under high pressure at 25MPa for 1-2 times, each time for 5 minutes, and homogenize the freshly squeezed dendrobium juice The juice is divided into PE / PA composite packaging bags, each bag is 50mL, and vacuum-sealed;

[0050] 3. Place the dendrobium fresh juice packaged in step 2 in ultra-high pressure processing equipment, hold the pressur...

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Abstract

The present invention discloses a method for guiding sterilization and preservation of freshly squeezed caulis dendrobii juice by using metagenomic sequencing data. The metagenomic sequencing analysisof freshly squeezed caulis dendrobii juice, ultra-high pressure caulis dendrobii juice and preservative and ultra-high pressure synergistically-treated caulis dendrobii juice is used as a basis to provide a sterilization and preservation scheme of nisin-natamycin and ultra-high pressure synergistically-treated caulis dendrobii juice. The method for guiding the sterilization and preservation of the caulis dendrobii juice by using the metagenomic sequencing data shortens test periods; at the same time, the technology reduces the ultra-high pressure sterilization pressure; besides, the product has advantages of being safe and hygienic, easy to digest and absorb, long in storage period, convenient for commercial circulation, etc.; and the method has great significance to the development of the fresh caulis dendrobii juice health-care drink.

Description

technical field [0001] The invention relates to a selection of a sterilizing and fresh-keeping scheme for freshly squeezed dendrobium juice, in particular to a method for guiding the sterilization and fresh-keeping of freshly squeezed dendrobium juice by using metagenomic sequencing data, belonging to the field of deep processing of agricultural products. Background technique [0002] Dendrobium was honored by the ancients as "the first of the nine Chinese celestial grasses" and "life-saving celestial grass", and modern people respectfully call it "the best of Chinese celestial grasses" and "healthy soft gold". Its main active ingredients are dendrobium polysaccharides, total alkaloids, free amino acids and various trace elements, etc. It has the functions of nourishing yin, detoxification, anti-cancer, anti-fatigue, anti-radiation, resistance to hypoxia, improving human immunity, and delaying aging. [0003] Since ancient times, Dendrobium has often been taken in fresh juic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L2/44A23L2/42
CPCA23L2/42A23L2/44A23V2002/00A23V2200/10A23V2300/46
Inventor 惠爱玲冯英鹏陈婧超刘淑芸
Owner HEFEI UNIV OF TECH
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