A method for the preparation of oligomeric proanthocyanidins by high-voltage pulsed electric field combined with ultra-high pressure micro-jet

Abstract

The invention discloses a method for preparing oligomeric proanthocyanidins through a high-voltage pulsed electric field in cooperation with an ultra-high pressure micro-jet. The method comprises thesteps of (1) dissolving proanthocyanidins in aqueous ammonia solution to obtain a proanthocyanidin solution with a mass concentration of 10-30%; (2) conducting high-voltage pulsed electric field treatment, wherein the proanthocyanidin solution is pumped into a pipeline provided with high-voltage electric and low-voltage electric stages and the high-voltage pulsed electric field is switched on; (3)conducting ultra-high pressure micro-jet treatment, wherein the proanthocyanidin solution is pumped into a micro-jet flow head of the ultra-high pressure micro-jet after the high-voltage pulsed electric field treatment and an oligomeric proanthocyanidin solution is obtained through the ultra-high pressure micro-jet treatment; (4) concentrating the oligomeric proanthocyanidin solution in vacuum, and spray-drying to obtain the oligomeric proanthocyanidins. The proanthocyanidin depolymerization process of the method has simple operation and high depolymerization efficiency, and is suitable for large-scale and continuous preparation of the oligomeric proanthocyanidins.

Description

technical field [0001] The invention belongs to the field of food, and relates to a method for preparing oligomeric proanthocyanidin powder in cooperation with a high-voltage pulsed electric field and an ultra-high-voltage micro-jet. Background technique [0002] Procyanidins (PC) is a bioflavonoid with a special molecular structure, which has anti-oxidation, anti-tumor, and blood pressure-lowering effects. PC is an oligomer with a polymerization degree of 2-10 and a polymerization degree of less than 4, which is polymerized by different amounts of catechin or epicatechin, and has high activity. Therefore, increasing the degree of polymerization in PC to less than 4 oligomeric proanthocyanidins can improve the quality of proanthocyanidin products. [0003] At present, the depolymerization of proanthocyanidins recorded in the literature mainly includes the following methods, one is oxidative degradation method, such as "a kind of oligomer proanthocyanidin and its preparation...

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Problems solved by technology

[0004] In summary, in the current reports, the oxidative degradation conditions are severe, which is easy to destroy the phenolic hydroxyl group in PC; the enzymatic method is mild, but the time is long and the efficiency is low; the efficiency of pulsed electric field and ultra-high pressure micro-jet treatment is low

And in the prior art, low-concentration sulfite solution is used to dissolve proanthocyanidins. After dissociation of oligomeric proanthocyanidins, salt ions such as sulfite and oligomeric proanthocyanidins are mixed together. Separation of oligomeric proanthocyanidins, the steps are cumbersome and the cost is high

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