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A kind of method for preparing oligomeric proanthocyanidins from litchi peel

A technology of oligomeric proanthocyanidins and lychee peel, which is applied in microorganism-based methods, biochemical equipment and methods, organic chemistry and other directions, can solve the problems of increasing purification difficulty, low degradation yield, waste of raw materials, etc. High depolymerization efficiency and time saving effect

Active Publication Date: 2022-03-22
SIRIO PHARMA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The temperature required for water extraction is high, which is easy to damage the structure of PC, and the extracted impurities are also more. The commonly used extraction solvent is a certain proportion of ethanol aqueous solution, which has better extraction efficiency, but requires a large amount and high cost.
At present, the methods for degrading high-polymeric proanthocyanidins at home and abroad are mainly chemical methods, such as acid hydrolysis. Although the process of acid hydrolysis is simple, the degradation rate is low, waste raw materials, and increase the difficulty of subsequent purification.

Method used

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  • A kind of method for preparing oligomeric proanthocyanidins from litchi peel
  • A kind of method for preparing oligomeric proanthocyanidins from litchi peel
  • A kind of method for preparing oligomeric proanthocyanidins from litchi peel

Examples

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Effect test

Embodiment 1

[0045] (1) Fermentation in one step: collect lychee skins, air-dry them and crush them, take 3 kg of litchi skin powder, put them in an iron barrel, add 15 times the mass of water, add 10 kg of sucrose, and mix to obtain fermentation raw materials. Add 50 g of Saccharomyces cerevisiae powder into 5 kg of sterile water containing 2 wt % sucrose, and incubate in a water bath at 37° C. for 30 min to activate. Pour the activated yeast liquid into the fermentation raw material, inoculate 2.8g of Lactobacillus plantarum powder, stir and mix well, leave it to ferment in a closed room at room temperature, take samples every 24 hours to measure the ethanol concentration and pH value, after 24 hours of fermentation, the ethanol concentration reaches 142.71 mg / mL, the pH dropped to 4.17, and the fermentation ended.

[0046] (2) Secondary fermentation: Sprinkle and inoculate 12g of Acetobacter pasteurianum powder on the surface of the fermented raw material after the first fermentation, w...

Embodiment 2

[0049] (1) Fermentation in one step: collect lychee skins, air-dry them and crush them, take 3 kg of litchi skin powders, put them in an iron bucket, add 20 times the quality of water, add 11 kg of sucrose, and mix to obtain fermentation raw materials. Add 50 g of Saccharomyces cerevisiae powder into 5 kg of sterile water containing 2 wt % sucrose, and incubate in a water bath at 37° C. for 30 min to activate. The activated yeast liquid is poured into the fermentation raw material, inoculated with 1.8g of Lactobacillus plantarum powder, stirred and mixed, sealed and left to ferment at room temperature. Samples were taken every 24 hours to measure the ethanol concentration and pH value. After 48 hours of fermentation, the ethanol concentration reached 158.79 mg / mL and the pH dropped to 4.02, and one fermentation was ended.

[0050] (2) Secondary fermentation: Sprinkle and inoculate 30g of Acetobacter pasteurianum powder on the surface of the fermented material after the first f...

Embodiment 3

[0053] (1) Fermentation in one step: collect lychee skins, air-dry them and crush them, take 3 kg of litchi skin powder, put them in an iron bucket, add 25 times the quality of water, add 12 kg of sucrose, and mix to obtain fermentation raw materials. Add 45g of Saccharomyces cerevisiae powder into 4.5kg of sterile water containing 2wt% sucrose, and incubate in a water bath at 37°C for 30 minutes to activate. Pour the activated yeast liquid into the fermentation raw material, inoculate 0.9g of Lactobacillus plantarum powder, stir and mix well, leave it to ferment in a closed room at room temperature, take samples every 24 hours to measure the ethanol concentration and pH value, after 72 hours of fermentation, the ethanol concentration reaches 172.43 mg / mL, the pH dropped to 3.87, and the fermentation ended.

[0054] (2) Secondary fermentation: Sprinkle and inoculate 25g of Acetobacter pasteurianum powder on the surface of the fermented material after the first fermentation, wi...

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Abstract

The invention discloses a method for preparing oligomeric proanthocyanidins from litchi peel. The invention mixes lychee skin powder, water and sugar source to obtain fermentation raw materials, inoculates yeast and lactic acid bacteria, seals and stands still for one fermentation; inoculates Acetobacter, stands still and performs second fermentation; filters, concentrates and extracts to obtain oligomerization Crude procyanidin extract; purified and dried to obtain oligomeric proanthocyanidins. The invention utilizes ethanol produced by yeast to increase the effective dissolution of proanthocyanidins in litchi skin; utilizes the acidic environment provided by lactic acid bacteria to maintain the stability of proanthocyanidins and improves extraction efficiency; utilizes acetic acid produced by acetic acid bacteria and enzymes produced by biological fermentation to make high polyproanthocyanidins The depolymerization efficiency is higher, the extraction yield of proanthocyanidins is higher, and the purity of the obtained oligomeric proanthocyanidins is higher. The method of the present invention uses yeast to produce ethanol, reduces costs, utilizes low concentration of ethanol to reduce the dissolution of lipid components, does not require degreasing treatment for subsequent purification, reduces procedures, and saves costs.

Description

technical field [0001] The invention relates to the technical field of natural plant extraction and processing, in particular to a method for preparing oligomeric proanthocyanidins from litchi peel. Background technique [0002] Proanthocyanidin (PC) is a kind of flavanoid substance with super antioxidant properties, which has been proven to help prevent and treat aging caused by free radicals, and has a good effect on the treatment of heart disease and cancer. However, due to the large amount of phenolic hydroxyl groups in PC, it is easily oxidized into anthocyanins. At the same time, PC is a mixture formed by the polymerization of different monomers, which contains a large amount of high polymer (PPC, the degree of polymerization is greater than 4), which is difficult to penetrate the biomembrane, so it cannot be absorbed by the human body. In addition, due to its special structure, PPC has low bioavailability. During the extraction and separation process, it is often com...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D311/62C12P7/08C12R1/865
CPCC07D311/62C12P7/08
Inventor 杨继国夏晚霞邓兆雯王云刘玮刘丹
Owner SIRIO PHARMA CO LTD
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