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A method for domesticating and screening selenium-enriched mushroom strains

A technology for selenium mushrooms and candidate mushrooms, which are applied in the fields of botanical equipment and methods, horticulture, agriculture, etc., and can solve the problems of low organic selenium content, which cannot meet the needs of food and medicine, easy to produce toxic side effects, and harmful to human health. , to protect human health, reduce inorganic selenium, and increase the content of organic selenium

Active Publication Date: 2020-06-30
玖硒(北京)生物科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The organic selenium content of the selenium-enriched mushrooms obtained by the above two methods is about 0.1ppm. The organic selenium content is too low to meet the needs of food and medicine, and the efficiency is low. Moreover, most of the selenium obtained by the above two methods is inorganic selenium. It is easy to produce toxic and side effects and is harmful to human health. Therefore, it is urgent to develop a new method for cultivating selenium-enriched mushrooms, which can greatly increase the content of organic selenium in mushrooms, improve production efficiency, avoid toxic and side effects, and protect human health.

Method used

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  • A method for domesticating and screening selenium-enriched mushroom strains

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Embodiment 1

[0021] According to the following method, the primary bacterial culture medium was prepared:

[0022] (1) Peel the potatoes, remove the bud eyes, weigh 200g, cut into 1-2g hob pieces, put them in a pot, add 1000ml of water, boil and then cook for 10min. Use 4-6 layers of gauze to filter the discarded residue, pour the filtrate back into the pot, add 20g of agar, stir with a glass rod while heating with a slow fire, and add 20g of glucose, 1.5g of potassium dihydrogen phosphate, and 1.5g of dipotassium hydrogen phosphate after the agar is dissolved , magnesium sulfate 3g, vitamin B110mg. Add water to make up to 1000ml, and pour it into a graduated cylinder while it is hot to obtain a liquid medium.

[0023] (2) Place the funnel on the clamp of the iron stand, connect the latex tube at the bottom of the funnel, clamp a spring clip on the tube as a control switch, and connect the glass tube with a pointed mouth under the tube. Pour the liquid medium into the funnel while it is ...

Embodiment 2

[0027] Acclimate and screen according to the following steps to obtain the first-class strains of selenium-enriched Pleurotus ostreatus:

[0028] (1) Select two Pleurotus ostreatus strains with strong stress resistance, fast growth and high yield as candidate Pleurotus ostreatus strains.

[0029] (2) Make the first-grade strain culture medium; The first-grade strain culture medium is PDA medium, and comprises the raw material component of following weight ratio in every 1000mlPDA culture medium: peeled potato 200g, glucose 20g, potassium dihydrogen phosphate 1.5g , dipotassium hydrogen phosphate 1.5g, magnesium sulfate 3g, vitamin B110mg, agar 20g, and the rest is water.

[0030] (3) Prepare 18 test tubes, and each group of 9 test tubes is divided into two groups, i.e. the first group and the second group, respectively in the first group and the second group of test tubes, subpackage 8ml of primary bacterial culture medium, and Add 30ppm, 60ppm, 90ppm, 120ppm, 150ppm, 180ppm,...

Embodiment 3

[0034] Acclimate and screen according to the following steps to obtain the first-class strains of selenium-enriched Pleurotus ostreatus:

[0035] (1) Select five Pleurotus ostreatus strains with strong stress resistance, fast growth and high yield as candidate Pleurotus ostreatus strains.

[0036](2) Make the first-grade strain culture medium; The first-grade strain culture medium is PDA medium, and comprises the raw material component of following weight ratio in every 1000mlPDA culture medium: peeled potato 200g, glucose 20g, potassium dihydrogen phosphate 1.5g , dipotassium hydrogen phosphate 1.5g, magnesium sulfate 3g, vitamin B110mg, agar 20g, and the rest is water.

[0037] (3) Prepare 45 test tubes, divide each group into five groups according to 9 test tubes, that is, the first group, the second group, the third group, the fourth group and the fifth group. The test tubes of the third group, the fourth group and the fifth group were filled with 8ml of primary strain cu...

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Abstract

The invention provides a method for cultivating selenium-enriched mushrooms. The method includes preparing candidate mushroom strains; manufacturing primary strain culture media; quantitatively separately packaging the primary strain culture media and adding sodium selenite solution with different degrees of concentration into the primary strain culture media to obtain selenium-containing culturemedia with different degrees of concentration; inoculating the candidate mushroom strains into the selenium-containing culture media with the different degrees of concentration, culturing the candidate mushroom strains and keeping the appropriate temperatures, humidity and potential of hydrogen unchanged; domesticating and screening the candidate mushroom strains to obtain the candidate mushroom strains with high selenium enrichment capacity. The candidate mushroom strains with the high selenium enrichment capacity are used as primary strains. The method has the advantages that conventional modes for cultivating selenium-enriched mushrooms are changed, accordingly, the content of organic selenium in mushrooms can be vigorously increased, and the production efficiency can be improved.

Description

technical field [0001] The invention relates to the technical field of modern agricultural cultivation, in particular to a method for domesticating and screening selenium-enriched fungus strains. Background technique [0002] Although the content of selenium (Se) in the human body is only 0.1-0.2ppm, it is an indispensable important element in life activities. Selenium has the effects of improving the body's immunity, promoting disease recovery, anti-cancer and anti-aging. The "Supply of Nutrients in Daily Diet and my country's Dietary Guidelines" recommended by the Chinese Nutrition Society stipulates that the daily necessary intake of selenium is 50 micrograms for adults and teenagers, and 20 (1-3 years old) to 40 (4-3 years old) for children. 6 years) micrograms, which is the lower limit of the necessary amount. According to the survey, the average selenium intake of adults in the country is only 26.6 micrograms per day, which is almost only 1 / 2 of the lower limit of the...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G18/00A01G18/20
Inventor 向华周山
Owner 玖硒(北京)生物科技有限公司