Antimicrobial fusion peptide as well as preparation method and application thereof

A technology that fuses peptides and peptide chains, applied in the field of antibacterial drugs, can solve problems such as lack of antibacterial activity and anticancer drugs that cannot inhibit bacterial growth, and achieve the effect of improving anticancer effects and reducing drug dosage

Active Publication Date: 2018-10-02
西安华企众信科技发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Anticancer drugs can inhibit the growth of cancer cells, but anticancer drugs usually cannot inhibit the growth of bacteria, that is, they do not have antibacterial activity

Method used

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  • Antimicrobial fusion peptide as well as preparation method and application thereof
  • Antimicrobial fusion peptide as well as preparation method and application thereof
  • Antimicrobial fusion peptide as well as preparation method and application thereof

Examples

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Embodiment 1

[0026] Such as Figure 1 to Figure 3 As shown, the present invention provides an antibacterial fusion peptide, which is SISLL-TAT in which the nitrogen terminal of TAT (47-57) is linked to BRCA1 (782-786), and its peptide chain sequence is: SISLLYGRKKRRQRRR.

Embodiment 2

[0028] Such as Figure 4 to Figure 6 As shown, the present invention provides an antibacterial fusion peptide. The peptide sequence of the antibacterial fusion peptide is TAT-SISLL with the carbon terminal of TAT (47-57) connected to BRCA1 (782-786), and its peptide chain sequence is: YGRKKRRQRRRSISLL.

[0029] The present invention also provides the preparation method of antibacterial fusion peptide in embodiment one and embodiment two, and this method is:

[0030] Using Wang resin as the carrier, Fmoc as the amino acid side chain protecting group, piperidine in DMF solution as the deprotection reagent, HBTU, HOBT and DIEA as the amino acid condensing agent, according to the sequence of the target antibacterial fusion peptide, from the carbon (C-) terminal Go to the nitrogen (N-) terminal to couple the corresponding amino acid; until the coupling of the nitrogen-terminal amino acid is completed, remove the Fmoc protecting group of the nitrogen-terminal amino acid, wash and dr...

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Abstract

The invention discloses an antimicrobial fusion peptide. The peptide chain sequence of the antimicrobial fusion peptide is SISLLYGRKKRRQRRR. The invention further provides another antimicrobial fusionpeptide of which the peptide chain sequence is YGRKKRRQRRRSISLL. The invention provides a preparation method of the antibacterial fusion peptide. The preparation method comprises the following steps:coupling corresponding amino acids from the carbon terminal to the nitrogen terminal according to the sequence of a target antibacterial fusion peptide; washing and blowing deprotection groups to dryness after coupling of the nitrogen-terminated amino acid is completed; cutting, carrying out suction filtration and fully washing and centrifuging by using ice diethyl ether to remove supernatant forthree times or above; dissolving the dried solid powder by using deionized water, separating, purifying, collecting main peak effluent and carrying out freeze drying to obtain the target antimicrobial fusion peptide. The invention further discloses application of the antimicrobial fusion peptide in medicines. According to the preparation method disclosed by the invention, the fusion peptide withan antibacterial effect is obtained on the basis of anticancer peptide; in addition, the antibacterial effect of the antimicrobial fusion peptide is close to the antibacterial effect of a powerful antimicrobial drug kanamycin, so that a basis is provided for further research and development of drugs with dual effects of resisting cancer and bacteria.

Description

technical field [0001] The invention relates to the technical field of antibacterial drugs, in particular to an antibacterial fusion peptide. Background technique [0002] There are three sources of existing antimicrobial peptides, natural product extraction, chemical synthesis and biological synthesis. [0003] About chemical synthesis: [0004] Amino acids are amphoteric substances, generally containing amino groups (-NH 2 ) and carboxyl (-COOH) are the basic raw materials for chemical synthesis of antimicrobial peptides. Researchers usually use amino groups that do not participate in the condensation reaction (-NH 2 ), carboxyl (-COOH) and side chains with active groups are protected, and after all amino acids are coupled according to the antimicrobial peptide sequence, the deprotection reaction is carried out. [0005] Cancer is a disease that damages health and is life-threatening due to the excessive proliferation and proliferation of abnormal cells in the body. A...

Claims

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Application Information

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IPC IPC(8): C07K19/00C07K1/06C07K1/04C07K1/20A61K38/08A61P31/04A61P35/00
CPCA61K38/00A61P31/04A61P35/00C07K7/06C07K2319/00Y02P20/55
Inventor 吕名秀卢奎买文鹏刘广斌王梦伟彭露
Owner 西安华企众信科技发展有限公司
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