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Method of producing pharmaceutical grade sodium hyaluronate from phytone

A technology of sodium hyaluronate and plant peptone, which is applied in the field of biological fermentation, can solve the problems of excessive endotoxin content, few sources of raw materials, and unstable quality, and achieve the effects of small nucleic acid content, avoiding the risk of germs, and reducing production costs

Active Publication Date: 2018-10-02
SHANDONG FOCUSFREDA BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] Aiming at the current problems of animal peptones carrying various viruses, less sources of raw materials, higher prices, unstable quality, and excessive endotoxin content, the present invention provides a method for producing pharmaceutical grade sodium hyaluronate using plant peptones. It uses plant protein to make peptone, which is used in the fermentation production of pharmaceutical grade hyaluronic acid. It uses alkaline protease to degrade plant protein into a protein that is conducive to the absorption and utilization of bacteria, and then removes endotoxin together with other fermentation medium components to achieve pharmaceutical Production requirements for grade hyaluronic acid

Method used

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  • Method of producing pharmaceutical grade sodium hyaluronate from phytone

Examples

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Effect test

Embodiment 1

[0040] In this example, the method for producing pharmaceutical-grade sodium hyaluronate using plant peptone includes a) using vegetable protein as a raw material to prepare a fermentation medium, b) using the prepared fermentation medium to prepare sodium hyaluronate by microbial fermentation,

[0041] 1. The preparation of fermentation medium comprises the steps:

[0042] (1) Dissolve the material in the dissolving tank, add 800L of water, start stirring, open the steam valve until the temperature rises to 75°C, close the steam valve, put 125kg of zein into the dissolving tank, and then open the steam valve to maintain the temperature of the material at 95°C and dissolve for 10 minutes ;

[0043] (2) Enzymolysis After the dissolution is completed, turn on the cold water and lower the temperature to 50°C, adjust the pH of the material to 8.5 with alkali, then add 0.8kg of protease for enzymolysis for 5 hours, and continue to add alkali during the enzymolysis to keep the pH at...

Embodiment 2

[0067] In this example, the method for producing pharmaceutical-grade sodium hyaluronate using plant peptone includes a) using vegetable protein as a raw material to prepare a fermentation medium, and b) using the prepared fermentation medium to prepare sodium hyaluronate by microbial fermentation. The preparation of fermentation medium comprises the steps:

[0068] (1) Dissolve the material in the dissolution tank, add 800L of water, start stirring, open the steam valve until the temperature rises to 90°C, close the steam valve, put 125kg of soybean protein into the dissolution tank, and then open the steam valve to maintain the temperature of the material at 100°C and dissolve for 30 minutes ;

[0069] (2) Enzymolysis After the dissolution is completed, turn on the cold water and lower the temperature to 40°C, adjust the pH of the material to 7 with alkali, then add 0.8kg of protease for enzymolysis for 10 hours, and keep adding alkali during the enzymolysis to keep the pH a...

Embodiment 3

[0077] In this example, the method for producing pharmaceutical-grade sodium hyaluronate using plant peptone includes a) using vegetable protein as a raw material to prepare a fermentation medium, and b) using the prepared fermentation medium to prepare sodium hyaluronate by microbial fermentation. The preparation of fermentation medium comprises the steps:

[0078] (1) Dissolve the material in the dissolution tank, add 800L of water, start stirring, open the steam valve until the temperature rises to 85°C, close the steam valve, put 125kg of wheat gluten flour into the dissolution tank, and then open the steam valve to maintain the temperature of the material at 95°C for dissolution 1h;

[0079] (2) Enzymatic hydrolysis After the dissolution is completed, turn on cold water and lower the temperature to 60°C, adjust the pH of the material to 10 with alkali, then add 0.8kg of protease for enzymatic hydrolysis for 1 hour, and continue to add alkali during the enzymatic hydrolysi...

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Abstract

The invention discloses a method of producing pharmaceutical grade sodium hyaluronate from phytone and relates to the technical field of biological fermentation. The method is characterized by comprising the steps of a) preparing a fermentation medium by taking a vegetable protein as a raw material, and b) preparing sodium hyaluronate from the prepared fermentation medium by a microbial fermentation method, wherein a preparation method of the fermentation medium comprises the steps of dissolution, enzymolysis, enzyme deactivation, filtration, carbon absorption, decarbonization, fermentation and sterilization. The peptone is prepared from the vegetable protein creatively and used for the fermentation production of pharmaceutical grade hyaluronic acid; the risk that sodium hyaluronate prepared by fermentation of bovine bone peptone carried a bovine spongiform encephalopathy virus in the prior art is avoided; the method is high in safety; raw materials are easy to obtain; the raw materialcost is greatly lowered; and the fermentation productivity is improved.

Description

technical field [0001] The invention relates to the technical field of biological fermentation, in particular to a method for producing medical-grade sodium hyaluronate by utilizing plant peptone. Background technique [0002] Hyaluronic acid (Hyaluronic acid, Hyaluronan, HA for short), also known as hyaluronic acid, is a straight-chain mucopolysaccharide composed of acetylamino deoxyglucose and glucuronic acid disaccharide repeating units. It is widely used in food, cosmetics and pharmaceutical fields. Wide range of applications. In the field of food, it is used as a raw material for health food to increase bone density or improve skin moisture; in the field of cosmetics, it is used as a moisturizing factor in skin care products, such as facial masks, essences, creams, and lotions; in the field of plastic surgery, it is used as a moisturizing factor in skin care products. Fillers are used to remove facial wrinkles, rhinoplasty, lip augmentation, etc.; in the field of medic...

Claims

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Application Information

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IPC IPC(8): C12P19/26
CPCC12P19/26
Inventor 张美霞杜帅孔祥一尤进茂康传利李取泉李庆李焕成李娟张梦益刘磊
Owner SHANDONG FOCUSFREDA BIOTECH CO LTD
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