Extraction method of Chinese yam glycoprotein
An extraction method and yam sugar technology, which are applied in the field of yam glycoprotein extraction, can solve problems such as glycoprotein, a single active component of yam, and achieve the effects of inhibiting tumors, improving purity, and reducing blood lipids.
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Embodiment 1
[0014] Embodiment 1, the present invention is described in detail below, a kind of extraction method of Chinese yam glycoprotein comprises following processing steps:
[0015] (1) Prepare an aqueous solution containing 0.1%-1% citric acid, 0.2%-0.8% sodium citrate, and 0.1%-0.5% ascorbic acid;
[0016] (2) Peel and slice fresh yam, and then add it to the aqueous solution in step (1), the mass ratio of yam to aqueous solution is 1:4;
[0017] (3) Grinding with a 120-mesh stone disc;
[0018] (4) Centrifuge three times with 80 mesh, 100 mesh, and 120 mesh in a centrifuge to remove fibers and black residue in the pores of yam to obtain the filtrate;
[0019] (5) Then add 0.02% immobilized compound protease to the filtrate, heat and stir at 45°C for 30 minutes, and filter out the immobilized compound protease;
[0020] (6) The filtrate is then separated by a decanter centrifuge to remove the starch to obtain a clarified liquid, and then 70% ethanol is used to precipitate the gly...
example 1
[0022] Example 1: Get 5 kilograms of fresh yams and peel them, add 20 kilograms of solutions containing 0.5% citric acid, 0.4% sodium citrate, and 0.2% ascorbic acid after peeling and slicing, grind with a stone disc mill with a 120-mesh filter screen installed, Then centrifuge three times with 80 mesh, 100 mesh, and 120 mesh nets in a centrifuge to obtain 21.2 kg of filtrate, add 4.2 g of immobilized composite protease to the filtrate, heat and stir at 45 ° C for 30 minutes, and filter out the immobilized composite protease. After being separated by a decanter centrifuge, 20.8 kg of clarified liquid was obtained, and the concentration of ethanol in the clarified liquid was adjusted to 70% with 95% ethanol. After standing for 12 hours, solid matter was obtained after centrifugation, and dried under reduced pressure to obtain 0.717 kg of glycoprotein with a purity of 96.7%. .
example 2
[0023] Example 2: Take 25 kg of fresh yam, peel it, slice it, add it to 100 kg of a solution containing 0.5% citric acid, 0.4% sodium citrate, and 0.2% ascorbic acid, grind it with a stone mill and install a 120-mesh filter, and then centrifuge The machine was separated three times with 80 mesh, 100 mesh, and 120 mesh nets to obtain 108.5 kg of filtrate. Add 21.7 g of immobilized compound protease to the filtrate, heat and stir at 45 ° C for 30 minutes, and filter out the immobilized compound protease. The filtrate was then subjected to sedimentation centrifugation 105.3 kg of clarified liquid was obtained by mechanical separation, and the ethanol concentration in the clarified liquid was adjusted to 70% with 95% ethanol, left to stand for 12 hours, centrifuged to obtain solid matter, dried under reduced pressure to obtain 3.78 kg of glycoprotein with a purity of 95.4%.
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