Method for rapid breeding of maize leafless tongue inbred lines with assistance of molecular markers

A molecular marker-assisted and molecular marker technology, applied in the field of molecular genetics, can solve the problems of long selection time, long cycle, low efficiency, etc., and achieve the effect of improving accuracy and saving time for line selection

Active Publication Date: 2018-10-09
BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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  • Abstract
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Problems solved by technology

It can be seen that using the conventional backcross transfer method, for the traits controlled by a recessive single gene, it is necessary to use backcross and self-cross alternate methods to accurately select the target traits, which takes a long time and low efficiency
In addition, only using conventional breeding methods generally requires more than 5 generations of backcrossing to basically restore the genetic background of the reincarnated parent, which takes a long time.

Method used

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  • Method for rapid breeding of maize leafless tongue inbred lines with assistance of molecular markers
  • Method for rapid breeding of maize leafless tongue inbred lines with assistance of molecular markers
  • Method for rapid breeding of maize leafless tongue inbred lines with assistance of molecular markers

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Experimental program
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Embodiment 1

[0027] Example 1 Screening of maize ligule lg1 gene SSR molecular markers

[0028] 1. Using the maize genome database MaizeGDB (http: / / www.maizegdb.org / ), select a total of 9 SSR molecular markers in the upstream and downstream intervals with a close genetic distance to the lg1 gene located at the end of the short arm of the second chromosome of maize.

[0029] Table 1 The positions and primer sequences of the upstream and downstream markers of the ligule gene lg1

[0030]

[0031]

[0032] Note: The position indicates the relative genetic position in IBM2 2008 Neighbors Frame 2 on the MaizeGDB website, where the lg1 gene is 50.9

[0033] Genomic DNA was extracted from the B73 ligule-less mutant rla1 and the inbred lines (Jing 2416 and Jing 92), and the above-mentioned molecular markers were used to optimize and screen PCR reaction conditions. Tongjing 2416 and Jing 92 are polymorphic, well-resolution SSR molecular markers that are close to the genetic distance of the lg1...

Embodiment 2

[0040] Example 2 Breeding of maize liguleless inbred lines

[0041] 1. Acquisition of hybrid offspring F1

[0042] In the summer of the first year, maize inbred line rla1 (non-recurrent parent, without ligule) was used as donor, and Jing 2416 (available from the Corn Research Center of Beijing Academy of Agriculture and Forestry Sciences) (recurrent parent, with ligule) was used as recipient. Combination F 1 Generation, Harvest F 1 generation of seeds.

[0043] The first generation in Hainan in the winter of the same year, planting F 1 generation seed, get F 1 Substitute maize plants.

[0044] 2. Obtain the BC1 generation by backcrossing once

[0045] (1) Backcross

[0046] The F1 generation plants were used as the female parent, and continued to backcross with the recurrent parent Jing 2416, and the seeds of the BC1 generation were harvested. In the winter of the same year, the second generation in Hainan planted the seeds of the BC1 generation population to obtain th...

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Abstract

The invention provides a method for rapid breeding of maize leafless tongue inbred lines with assistance of molecular markers. By virtue of two SSR molecular markers closely linked to a maize leaflesstongue gene lg1, a maize leafless tongue inbred line is obtained by hybridization, three backcrossing and molecular marker assisted foreground and background selection, and selfing. In the method, the heterozygous genotype Lg1lg1 is identified and screened in the process of backcrossing, thereby eliminating the step of selfing of backcross descendants, and compared with conventional breeding methods, the accuracy of selection is enhanced, and nearly half of the line selection time can be saved. At the same time, in view of the problem that the background recovery cycle of a traditional backcrossing method is long, the method, in combination with a method in which molecular markers assist in background selection, can be used to obtain a leafless tongue improved line with genetic backgroundbasically recovering to recurrent parent after 3 generations of backcrossing and 1 generation of selfing only.

Description

technical field [0001] The invention relates to the field of molecular genetics, in particular to a method for rapid selection of maize ligule inbred lines assisted by molecular markers, in particular, it involves the use of SSR molecular markers linked to maize ligule gene Ig1 for assisted breeding. Background technique [0002] In recent years, with the breeding goals of maize tolerance to dense planting and high light efficiency, compact plant type and leaf upshoot have become important phenotypic traits that maize breeders pay attention to. Due to the disappearance of ligule auricles, phimosis of leaf sheaths, erect leaves, large photosynthetic area, and high light energy utilization efficiency, the ligule-less germplasm is an important resource for the breeding of hybrids resistant to dense planting. There are dominant and recessive traits of liguleless in maize that have been found so far. The lg1 gene is a recessive liguleless gene located at the end of the short arm ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895C12Q1/686
CPCC12Q1/686C12Q1/6895C12Q2525/151
Inventor 宋伟赵久然苏爱国邢锦丰张如养王元东王继东王帅帅
Owner BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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