Preparing method of stem-cell-carried fibrin glue used for promoting wound healing

A fibrin gel, wound healing technology, applied in pharmaceutical formulations, medical science, bandages, etc., can solve the problems of unable to fill cells, easy to form sunken scars in wounds, long recovery time, etc., to accelerate the coagulation reaction, promote Effects of endothelial cell growth, accelerated healing and repair

Inactive Publication Date: 2018-10-12
SOUTHWEST JIAOTONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, it cannot replace the missing cells in the wound, and the wound is prone to form sunken scars, which takes a long time to heal

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] A preparation method of stem cell-loaded fibrin gel capable of promoting wound healing, the steps are as follows:

[0015] A, dissolve bovine fibrinogen with physiological saline, be made into the solution that concentration is 5mg / mL; Add vascular endothelial growth factor again, obtain mixed solution one, the concentration of vascular endothelial growth factor in mixed solution one is 50ng / mL;

[0016] B. Use CaCl with a concentration of 0.33mol / mL 2 The solution dissolves human thrombin to prepare a human thrombin solution with a concentration of 30iu / mL; then add mesenchymal stem cells to obtain a mixed solution 2, and the concentration of the mesenchymal stem cells in the mixed solution 2 is 60×10 4 a / mL;

[0017] C. Use a 200 μL pipette gun to mix the mixed solution 1 and the mixed solution 2 at a volume ratio of 1:5, and drop them on the polydimethylsiloxane substrate; then, put them in an incubator at 37°C and let them stand for 4 hours , that is.

[0018] Th...

Embodiment 2

[0020] A preparation method of stem cell-loaded fibrin gel capable of promoting wound healing, the steps are as follows:

[0021] A, dissolve bovine fibrinogen with physiological saline, be made into the solution that concentration is 10mg / mL; Add vascular endothelial growth factor again, obtain mixed solution one, the concentration of vascular endothelial growth factor in mixed solution one is 100ng / mL;

[0022] B. Use CaCl with a concentration of 0.44mol / mL 2 The solution dissolves human thrombin to make a human thrombin solution with a concentration of 50iu / mL; then add mesenchymal stem cells to obtain a mixed solution 2, the concentration of mesenchymal stem cells in the mixed solution 2 is 70×10 4 a / mL;

[0023] C. Use a 200 μL pipette gun to mix the mixed solution 1 and the mixed solution 2 at a volume ratio of 1:5, and drop them on the polydimethylsiloxane substrate; then, put them in an incubator at 37°C and let them stand for 8 hours , that is.

[0024] The operati...

Embodiment 3

[0026] A preparation method of stem cell-loaded fibrin gel capable of promoting wound healing, the steps are as follows:

[0027] A, dissolve bovine fibrinogen with physiological saline, be made into the solution that concentration is 30mg / mL; Add vascular endothelial growth factor again, obtain mixed solution one, the concentration of vascular endothelial growth factor in mixed solution one is 150ng / mL;

[0028] B. Use CaCl with a concentration of 0.66mol / mL 2 The solution dissolves human thrombin to make a human thrombin solution with a concentration of 70iu / mL; then add mesenchymal stem cells to obtain a mixed solution 2, the concentration of the mesenchymal stem cells in the mixed solution 2 is 80×10 4 a / mL;

[0029] C. Use a 200 μL pipette gun to mix the mixed solution 1 and the mixed solution 2 at a volume ratio of 1:5, and drop them on the polydimethylsiloxane substrate; then, put them in an incubator at 37°C and let them stand for 12 hours , that is.

[0030] The op...

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PUM

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Abstract

The invention discloses a preparing method of stem-cell-carried fibrin glue capable of promoting wound healing. The preparing method comprises the following steps that A, bovine fibrinogens are dissolved by normal saline to be prepared into a solution with the concentration being 5-30 mg / mL, then a vascular endothelial growth factor is added to obtain a first mixed solution, and the concentrationof the vascular endothelial growth factor in the first mixed solution is 50-150 ng / mL; B, human thrombin is dissolved by a CaCl2 solution with the concentration being 0.33-0.66 mol / mL to be prepared into a human thrombin solution with the concentration being 30-70 iu / mL, then mesenchymal stem cells are added to obtain a second mixed solution, and the concentration of the mesenchymal stem cells inthe second mixed solution is (60-80)*104 piece / mL; and C, the first mixed solution and the second mixed solution are mixed to be dropped on a polydimethylsiloxane substrate by a 200 [mu]L pipette according to the volume ratio of 1:5, then the polydimethylsiloxane substrate is placed in a 37 DEG C incubator, still standing is conducted for 4-12 hours, and the stem-cell-carried fibrin glue is obtained. The stem-cell-carried fibrin glue prepared through the preparing method can induce differentiation of the stem cells into endothelialization, promote proliferation of endothelial cells and accelerate healing and repair of skin tissues, and wounds heal quickly.

Description

technical field [0001] The invention relates to a preparation method of stem cell-loaded fibrin gel for promoting wound healing. Background technique [0002] With the development of science and technology, there are more and more applications of hydrogels in tissue cell culture, drug sustained-release carriers and microcapsules. Hydrogels include natural hydrogels and synthetic hydrogels. Fibrin glue is a kind of natural hydrogel, which can be well accepted by the human body and has no immune reaction; fibrin gel loaded with Ca2+ can be used as a hemostatic material and has a good hemostatic effect on wounds. However, it cannot replace the missing cells in the wound, and the wound is prone to form sunken scars, which take a long time to heal. Contents of the invention [0003] The purpose of the present invention is to provide a preparation method of stem cell-loaded fibrin gel for promoting wound healing. The stem cell-loaded fibrin gel prepared by the method can induce...

Claims

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Application Information

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IPC IPC(8): A61L26/00
CPCA61L26/0042A61L26/0047A61L26/0057A61L26/0066A61L26/008A61L2300/412A61L2400/04C08L89/00
Inventor 陈俊英高霞丽谭建英魏来李莉黄楠
Owner SOUTHWEST JIAOTONG UNIV
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