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Microalgae sterile culture device and method suitable for mixotrophic culture

A culture device and microalgae technology, applied in the direction of microorganism-based methods, enzymology/microbiology devices, biochemical cleaning devices, etc., can solve the problems of easy contamination of miscellaneous bacteria, so as to avoid contamination of miscellaneous bacteria and improve biomass production rate effect

Pending Publication Date: 2018-10-12
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] In view of the problems existing in the above-mentioned prior art, the present invention aims to provide a microalgae aseptic culture device and culture method suitable for concurrent culture. The culture device of the present invention can use organic carbon sources to carry out concurrent culture of microalgae , improve the biomass yield of microalgae, and solve the problem of easy contamination of miscellaneous bacteria in the process of microalgae culture and sampling

Method used

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  • Microalgae sterile culture device and method suitable for mixotrophic culture
  • Microalgae sterile culture device and method suitable for mixotrophic culture
  • Microalgae sterile culture device and method suitable for mixotrophic culture

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Embodiment 1

[0051] Such as Figure 1-3 Described, a microalgae aseptic culture device suitable for concurrent cultivation, the culture device comprises: a culture tank top cover 1, a tank body, a base 4, the upper and lower bottom surfaces of the tank body are open, and the culture tank The top cover 1 is detachably arranged at the opening of the upper bottom surface of the tank body, the lower bottom surface of the tank body is detachably connected with the base 4 , the bottom of the base 4 is a funnel-shaped structure, and the lower end of the funnel-shaped structure is provided with a discharge port 18 .

[0052] The tank body is composed of double walls, wherein the outer wall 2 is made of reflective material, the inner wall 3 is made of transparent material, the reflective material is a reflective one-way see-through film, and the transparent material is polyacrylic methyl acrylate, and the outer wall 2 of the tank is detachably pasted to the outer surface of the inner wall.

[0053...

Embodiment 2

[0061] A method for cultivating microalgae suitable for concurrent culture, comprising the steps of:

[0062] (1) thoroughly cleaning and disinfecting the microalgae aseptic culture device described in embodiment 1;

[0063] (2) Mix the isolated and purified strain of Septoria splendens with the sterile BG11 culture solution that added glucose, sucrose, soluble starch, sodium acetate, and sodium citrate as carbon sources, and add it to the culture medium under aseptic conditions. In the tank, the seeding density of algae cells was 10 6 cells / ml;

[0064] (3) The reaction system is placed in an artificial climate chamber, and the ambient temperature is set to 25°C;

[0065] (4) Adjust the initial light intensity to 2000 Lux, and increase the light intensity in the same proportion as the algae cell density increases, so that the average light intensity in the culture medium is stable;

[0066] (5) During the adjustment period, the logarithmic growth period and the stable peri...

Embodiment 3

[0070] A method for cultivating microalgae suitable for concurrent culture. The difference is that the tank body of the cultivating device has only an inner wall made of a transparent material, and does not include an outer wall made of a reflective material.

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Abstract

The invention relates to a microalgae sterile culture device and method suitable for mixotrophic culture,and belongs to the field of microalgae culture devices. The culture device comprises a culturetank top cover,a tank body,a base and a light source; the upper and lower bottom surfaces of the tank body are open,the culture tank top cover is detachably arranged at the opening of the upper bottomsurface of the tank body,the lower bottom surface of the tank body is detachably connected with the base,the bottom of the base is of a funnel-shaped structure,and the lower end of the funnel-shapedstructure is provided with a discharging port; the light source is arranged in the tank body and connected with the culture tank top cover,and the tank body is composed of a reflective outer-layer wall and a transparent inner-layer wall; the base is internally laid with an air distribution structure,the air distribution structure is composed of two strip-shaped air distribution plates connected inseries,and one air distribution plate is connected with an air inlet. According to the microalgae sterile culture device and method suitable for the mixotrophic culture,by jointly applying the built-in light source with adjustable intensity and the inner reflection outer-layer wall of the culture tank,the efficiency of utilizing light energy is improved to the greatest degree,the photosynthesis efficiency of microalgae is also improved,and the purposes of saving the cost and increasing the yield are well achieved.

Description

technical field [0001] The invention relates to the field of microalgae culture devices, in particular to an aseptic culture device and a culture method capable of concurrently cultivating microalgae. Background technique [0002] Microalgae have fast growth, high oil content, and can assimilate CO in the air 2 As well as many advantages such as organic carbon source in wastewater, microalgae are being widely concerned all over the world as raw materials for the production of third and fourth generation biodiesel. However, at present, the low yield of microalgae biomass and high production cost limit the industrialization of microalgae biodiesel. The use of organic carbon sources for co-cultivation can greatly promote the growth of microalgae and increase the yield of biomass, but there are problems that it is easy to contaminate bacteria and affect the yield. Most of the existing microalgae cultivation devices are designed for the autotrophic cultivation of microalgae, wh...

Claims

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Application Information

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IPC IPC(8): C12M1/00C12M1/04C12N1/12C12R1/89
CPCC12M21/02C12M23/20C12M23/38C12M29/06C12M31/10C12N1/12C12M31/04
Inventor 纪雁李强樊祥宇高娟李玉梅
Owner UNIV OF JINAN
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