Application, kit and detection method of novel molecular marker non-coding RNA LINC00671 for prognosis of kidney cancer

A molecular marker and non-coding technology, applied in the field of tumor molecular biology, can solve the problems that have not been reported, and achieve the effect of improving survival rate, improving postoperative quality of life, and far-reaching clinical significance

Inactive Publication Date: 2018-10-12
THE FIRST AFFILIATED HOSPITAL OF ZHENGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Whether LINC00671 can be used as a new tumor marker to predict the prognosis of renal cancer patients has not been reported

Method used

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  • Application, kit and detection method of novel molecular marker non-coding RNA LINC00671 for prognosis of kidney cancer
  • Application, kit and detection method of novel molecular marker non-coding RNA LINC00671 for prognosis of kidney cancer
  • Application, kit and detection method of novel molecular marker non-coding RNA LINC00671 for prognosis of kidney cancer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1: The application of non-coding RNA LINC00671, a novel molecular marker for prognosis of kidney cancer, in the preparation of preparations for predicting the prognosis of kidney cancer patients. The marker non-coding RNA LINC00671 is used to prepare preparations for the prognosis of kidney cancer patients to predict the condition of kidney cancer patients.

Embodiment 2

[0021] Example 2: Preparation of reagents for detecting the expression of non-coding RNA LINC00671 for the preparation of a kit for the prognosis of renal cancer patients (for 30 reactions) 1. Trizo 120ml

[0022] 2. Inhibit RNA degradation solvent 40ml;

[0023] 3. Chloroform 80ml;

[0024] 4. 80ml of isopropanol;

[0025] 5. DEPC water 10ml;

[0026] 6. 100 μl of a mixture of 10× random primers and Oligo dT primers;

[0027] 7. 150 μl of 5× reverse transcription reaction buffer;

[0028] 8. 100μl of 10mM deoxyribonucleotide triphosphate base dNTP containing Mg2+;

[0029] 9. 200U / μl M-MLV reverse transcriptase 50μl;

[0030] 10. SYBR Green qPCR Mix 500μl;

[0031] 11. 3 μM target gene LINC00671 specific primer (its sequence is shown in SEQ NO:2 and SEQ NO:3) 50 μl;

[0032] 12. 50 μl of 3 μM internal reference gene TUBA1A-specific primer (its sequence is shown in SEQ NO:4 and SEQ NO:5).

Embodiment 3

[0033] Example 3: Detection of non-coding RNA LINC00671 in tissue samples

[0034] 1. Collect the kidney cancer or normal control tissues to be tested, wash them with normal saline, put them into a cryopreservation tube filled with a solvent that inhibits RNA degradation, and put them in a -80°C refrigerator for later use.

[0035] 2. Extraction of RNA in tissues:

[0036] (1) Add liquid nitrogen to the mortar first, then cut the tissue into small pieces and grind it into powder in liquid nitrogen, take 100mg of tissue powder with a liquid nitrogen pre-cooled spoon and add it to the EP tube filled with 1ml of Trizol solution In the process, the total volume of the tissue powder should not exceed 10% of the volume of the Trizol used, and it should be fully mixed evenly;

[0037] (2) Leave at room temperature for 5 minutes, then add 20 μl of chloroform, cap the EP tube tightly and shake vigorously for 0.5 minutes, then centrifuge at 12,000 rpm for 10 minutes;

[0038] (3) Take...

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Abstract

The invention relates to application, a kit and a detection method of novel molecular marker non-coding RNA LINC00671 for prognosis of kidney cancer. Real-time fluorescence quantitative PCR (Polymerase Chain Reaction) and survival curve analysis show that the non-coding RNA LINC00671 derived from kidney cancer tissues is related with a survival rate of a patient, the content is higher and the survival rate is higher. The method provides powerful technical support for predicting prognosis survival ratio of kidney cancer patients, the postoperative life quality of the kidney cancer patients is facilitated, the developing of a postoperative treatment scheme is facilitated, the survival rate is increased, and the application, the kit and the detection method of the novel molecular marker non-coding RNA LINC00671 for prognosis of the kidney cancer disclosed by the invention have profound clinical significance.

Description

technical field [0001] The invention belongs to the field of tumor molecular biology, and in particular relates to an application, a kit and a detection method of a novel molecular marker non-coding RNA LINC00671 for prognosis of kidney cancer. Background technique [0002] Renal cancer is a malignant tumor derived from the urinary tubular epithelial system of the renal parenchyma. It is a common disease and a high incidence of the urinary system. The incidence of renal cancer accounts for a high proportion of human malignant tumors, accounting for about 2% of adult malignant tumors. 3%, accounting for 80% to 90% of adult renal malignancies. The rich blood supply of the kidney determines the high metastases of renal cancer in the early stage. About 25% of renal cancer patients have tumor metastasis, and a large part of patients are found to have tumor metastasis when they are first diagnosed. Statistics show that the number of patients dying of renal cancer is increasing yea...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886C12Q1/6851C12N15/11
CPCC12Q1/6851C12Q1/6886C12Q2600/118C12Q2600/158C12Q2600/178C12Q2531/113C12Q2563/107C12Q2545/101C12Q2561/113
Inventor 田田
Owner THE FIRST AFFILIATED HOSPITAL OF ZHENGZHOU UNIV
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