Method for rapidly cultivating Bletilla striata seedlings under natural light

Abstract

The invention provides a method for rapidly cultivating Bletilla striata seedlings under natural light, and relates to the technical field of plant bioengineering. The method includes the following steps: (1) selecting mature capsules of Bletilla striata as explants; (2) inoculating the capsules on a germination medium after disinfection treatment, seeds turning to green after 7-10 days, and protocorms being formed after 40-50 days; (3) inoculating the protocorms on a subculture multiplication and differentiation medium to cultivate; and (4) cutting cluster buds off multiplicated and differentiated seedlings, performing subculture multiplication, inoculating seedlings whose plant heights are above 3 cm to seedling hardening and rooting mediums, and transplanting the seedlings when the seedlings grow to 5-7 cm. 1-5 g / L of self-made artemisia argyi dry leaf powder is added in subculture multiplication and rooting culture phases; the cultivation process of test tube seedlings is to cultivate the seedlings under outdoor natural light; and therefore, the stress resistance of tissue culture seedlings can be strengthened, production costs can be reduced, a large amount of sterile proliferating seedlings can be obtained, and the seedlings can be put into production one year in advance under artificially controlled conditions; and compared with traditional breeding methods, the method has a transplanting survival rate increased by about 30% and a production cost reduced by 20%, and is suitable for large scale production of seedlings.

Description

technical field [0001] The invention belongs to the technical field of plant bioengineering, and in particular relates to a method for quickly cultivating bletilla striata seedlings under natural light. Background technique [0002] Bletilla striata (Bletilla striata) is a perennial herbaceous tuber plant of the genus Bletilla striata in the family Orchidaceae, also known as purple orchid root. There are 9 populations in the world, and 4 populations in my country. It is a multifunctional plant that integrates medicine, beauty, weight loss and ornamental , plant height 30-60cm. The white root of Bletilla striata has the effects of astringent and hemostasis, detumescence and muscle growth. Bletilla striata has a higher content of medicinal components. Bletilla striata flowers have purple, white, yellow flowers and other colors. Bletilla striata has beautiful plant shape, bright colors and high ornamental value. It can be used as cut flowers and potted plants. The germination...

AI Technical Summary

Problems solved by technology

In order to solve the technical problems of bletilla striata seedling breeding, break the bottleneck of seedlings and the limitation of low survival rate of transplanting

[0003] In recent years, people have adopted stem division and tissue culture for propagation, but due to division, the waste of raw materials is large, the germination rate is low, the wound is susceptible to disease, and the stress resistance decreases, which is difficult to meet the market demand; and the existing group of bletilla striata cultivation technology, with fruit pods as explants, multiplication and rooting culture are cultivated indoors, rooted seedlings are weak, poor resistance, low transplant survival rate, high production cost, difficult to form large-scale production